Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,018,973	A→G	N40S (AAT→AGT)	ubiE →	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,018,973	0	A	G	100.0%	215.7 / NA	62	N40S (AAT→AGT)	ubiE	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/31);  total (31/31)

GATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAATGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGG  >  NC_000913/4018838‑4019107                                                                                                                                        |                                                                                                                                       gatgaGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGAt                                                                                                                                    <  1:276943/140‑1 (MQ=255)    gaGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTg                                                                                                                                 <  2:919418/140‑1 (MQ=255)     aGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTCTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGa                                                                                                                                >  2:171452/1‑140 (MQ=255)       cAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATg                                                                                                                              <  1:1281636/140‑1 (MQ=255)            aTTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCAtt                                                                                                                         <  2:708045/140‑1 (MQ=255)             ttGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCAttt                                                                                                                        <  1:267575/140‑1 (MQ=255)               gagaTGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTgg                                                                                                                      >  1:974234/1‑140 (MQ=255)                    ggtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTc                                                                                                                 >  1:522245/1‑140 (MQ=255)                    ggtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTc                                                                                                                 >  1:48323/1‑140 (MQ=255)                    ggtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTc                                                                                                                 >  2:979919/1‑140 (MQ=255)                    ggtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTc                                                                                                                 >  1:1020784/1‑140 (MQ=255)                     gtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCa                                                                                                                >  2:348590/1‑140 (MQ=255)                     gtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCa                                                                                                                <  2:638591/140‑1 (MQ=255)                     gtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCa                                                                                                                >  2:1221377/1‑140 (MQ=255)                     gtggATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCa                                                                                                                >  1:638591/1‑140 (MQ=255)                      tggGTAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCAt                                                                                                               >  2:220066/1‑140 (MQ=255)                         aTAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGt                                                                                                            <  1:617035/140‑1 (MQ=255)                             gTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTtgt                                                                                                        >  1:103071/1‑140 (MQ=255)                               cacaAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTgg                                                                                                      >  2:1373023/1‑140 (MQ=255)                                acaAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGa                                                                                                     >  1:231879/1‑140 (MQ=255)                                acaAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGa                                                                                                     >  1:303937/1‑140 (MQ=255)                                  aaGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAg                                                                                                   <  2:522245/140‑1 (MQ=255)                                       acgacgCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGAtt                                                                                              >  1:75334/1‑140 (MQ=255)                                        cgacgCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTc                                                                                             >  1:375442/1‑140 (MQ=255)                                         gacgCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCa                                                                                            <  1:1221377/140‑1 (MQ=255)                                         gacgCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCa                                                                                            <  2:571268/140‑1 (MQ=255)                                          acgCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCAc                                                                                           >  1:1478931/1‑140 (MQ=255)                                            gCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACga                                                                                         <  2:375442/140‑1 (MQ=255)                                              aCTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACgatt                                                                                       <  2:303937/140‑1 (MQ=255)                                              aCTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACgatt                                                                                       <  1:979919/140‑1 (MQ=255)                                                 ttGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACgattgat                                                                                    <  2:75334/140‑1 (MQ=255)                                                       ttCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAgc                                                                              <  1:522405/140‑1 (MQ=255)                                                          aGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAgcggc                                                                           >  1:837883/1‑140 (MQ=255)                                                          aGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAgcggc                                                                           >  1:576116/1‑140 (MQ=255)                                                              cGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGAGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTAc                                                                       >  1:874258/1‑140 (MQ=255)                                                               gTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACg                                                                      >  1:1071759/1‑140 (MQ=255)                                                                 cgcgAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGcc                                                                    <  2:1313457/140‑1 (MQ=255)                                                                 cgcgAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGcc                                                                    <  1:803993/140‑1 (MQ=255)                                                                          aCAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGa                                                           <  1:348590/140‑1 (MQ=255)                                                                          aCAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGa                                                           <  2:1071759/140‑1 (MQ=255)                                                                          aCAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGa                                                           <  2:576116/140‑1 (MQ=255)                                                                          aCAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGa                                                           <  1:220066/140‑1 (MQ=255)                                                                           cAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGAc                                                          <  1:1060269/140‑1 (MQ=255)                                                                           cAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGAc                                                          <  2:1404771/140‑1 (MQ=255)                                                                           cAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGAc                                                          <  2:1336185/140‑1 (MQ=255)                                                                               aaGCGGATATGGTCGCCCACGTTTTCTATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTg                                                      <  2:1291097/140‑1 (MQ=255)                                                                               aaGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTg                                                      <  2:103071/140‑1 (MQ=255)                                                                                   ggATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTg                                                      >  2:653158/1‑136 (MQ=255)                                                                                   ggATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTg                                                      <  1:653158/136‑1 (MQ=255)                                                                                           tCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATctggct                                          <  1:358896/140‑1 (MQ=255)                                                                                                            tCCGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCt                         >  2:255646/1‑140 (MQ=255)                                                                                                             ccGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTg                        >  2:834047/1‑140 (MQ=255)                                                                                                              cGTGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGa                       <  1:834047/140‑1 (MQ=255)                                                                                                                tGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACa                     >  2:1229607/1‑140 (MQ=255)                                                                                                                tGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACa                     >  1:1187826/1‑140 (MQ=255)                                                                                                                tGGCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACa                     >  1:527348/1‑140 (MQ=255)                                                                                                                 ggCATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAg                    <  2:868560/140‑1 (MQ=255)                                                                                                                   cATCAAAATACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCg                  >  1:490070/1‑140 (MQ=255)                                                                                                                        aaaTACGATGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCGAAAtt             >  2:163091/1‑140 (MQ=255)                                                                                                                                tGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGcc     <  2:490070/140‑1 (MQ=255)                                                                                                                                tGTCATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGcc     >  2:788736/1‑140 (MQ=255)                                                                                                                                   cATGAGTGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTgg  >  2:822900/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GATGAGCAGGCATTGAGATGGTGGATAAGTCACAAGAAACGACGCACTTTGGTTTTCAGACCGTCGCGAAGGAACAAAAAGCGGATATGGTCGCCCACGTTTTCCATTCCGTGGCATCAAAATACGATGTCATGAATGATTTGATGTCATTTGGTATTCATCGTTTGTGGAAGCGATTCACGATTGATTGCAGCGGCGTACGCCGTGGGCAGACCGTGCTGGATCTGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGG  >  NC_000913/4018838‑4019107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑