Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,023,265	T→C	L177P (CTG→CCG)	tatC →	twin arginine protein translocation system ‑ TatC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,023,265	0	T	C	100.0%	254.0 / NA	73	L177P (CTG→CCG)	tatC	twin arginine protein translocation system ‑ TatC protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/36);  total (37/36)

GCTGGCATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCTGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGAT  >  NC_000913/4023128‑4023400                                                                                                                                          |                                                                                                                                        gCTGGCATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGtgccgc                                                                                                                                       >  2:1275322/1‑140 (MQ=255)  cTGGCATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTgccgct                                                                                                                                      <  2:1311371/140‑1 (MQ=255)    ggCATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCcgctgt                                                                                                                                    <  1:1260176/140‑1 (MQ=255)     gCATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCcgctgtg                                                                                                                                   <  1:1443674/140‑1 (MQ=255)      cATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCcgctgtgc                                                                                                                                  <  1:1228962/140‑1 (MQ=255)         ttGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTgg                                                                                                                               <  2:1447093/140‑1 (MQ=255)          tGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGa                                                                                                                              <  2:1449073/140‑1 (MQ=255)          tGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGa                                                                                                                              <  1:1458042/140‑1 (MQ=255)           ggCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGAt                                                                                                                             <  1:1275322/140‑1 (MQ=255)            gCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATg                                                                                                                            <  2:747951/140‑1 (MQ=255)                      cAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCt                                                                                                                  >  1:560198/1‑140 (MQ=255)                      cAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCt                                                                                                                  >  2:260083/1‑140 (MQ=255)                      cAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCt                                                                                                                  <  2:560198/140‑1 (MQ=255)                        aTACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCg                                                                                                                >  1:1442848/1‑140 (MQ=255)                             gcgcCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCaga                                                                                                           >  2:908613/1‑140 (MQ=255)                              cgcCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCagaa                                                                                                          >  1:926291/1‑140 (MQ=255)                              cgcCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCagaa                                                                                                          >  1:911873/1‑140 (MQ=255)                                  ggAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACt                                                                                                      <  1:260083/140‑1 (MQ=255)                                         gTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCaa                                                                                               >  2:804584/1‑140 (MQ=255)                                             aGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCaaaaaa                                                                                           <  2:759458/140‑1 (MQ=255)                                                  tCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGAc                                                                                                       >  2:1017033/1‑123 (MQ=255)                                                  tCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGAc                                                                                                       <  1:1017033/123‑1 (MQ=255)                                                  tCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGccc                                                                                      <  1:804584/140‑1 (MQ=255)                                                    cACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGt                                                                                    <  1:1052270/140‑1 (MQ=255)                                                    cACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGt                                                                                    <  2:911873/140‑1 (MQ=255)                                                    cACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGAATTGCCTCGCCAGAAGACTTACGCAAAAAACGCCCGt                                                                                    <  1:409352/140‑1 (MQ=255)                                                          cATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGc                                                                              >  2:315734/1‑140 (MQ=255)                                                          cATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGc                                                                              <  1:1304629/140‑1 (MQ=255)                                                           aTCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAAc                                                                                          <  1:499890/127‑1 (MQ=255)                                                           aTCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAAc                                                                                          >  2:499890/1‑127 (MQ=255)                                                           aTCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCt                                                                             >  2:1378308/1‑140 (MQ=255)                                                                 aGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCtggttgg                                                                       >  2:293391/1‑140 (MQ=255)                                                                             ttCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGt                                                           <  1:64769/140‑1 (MQ=255)                                                                              tCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGt                                                           >  1:524199/1‑139 (MQ=255)                                                                              tCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGt                                                           <  2:524199/139‑1 (MQ=255)                                                                              tCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTc                                                          >  1:1195839/1‑140 (MQ=255)                                                                                gTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCgg                                                        >  2:923364/1‑140 (MQ=255)                                                                                gTTATGGCGCTGTTTATGGCGTCTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTTGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCgg                                                        >  2:288766/1‑140 (MQ=255)                                                                                     ggCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGa                                                      >  2:93130/1‑137 (MQ=255)                                                                                     ggCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGa                                                      <  1:93130/137‑1 (MQ=255)                                                                                      gcgcTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGtt                                                  >  1:839250/1‑140 (MQ=255)                                                                                       cgcTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTg                                                 <  2:839250/140‑1 (MQ=255)                                                                                         cTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCggg                                                       >  2:674900/1‑132 (MQ=255)                                                                                         cTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCggg                                                       <  2:430398/132‑1 (MQ=255)                                                                                         cTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCggg                                                       >  1:430398/1‑132 (MQ=255)                                                                                         cTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCggg                                                       <  1:674900/132‑1 (MQ=255)                                                                                         cTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCt                                               >  1:766212/1‑140 (MQ=255)                                                                                         cTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCt                                               >  2:1350477/1‑140 (MQ=255)                                                                                           gTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGa                                             <  2:1442848/140‑1 (MQ=255)                                                                                            tttATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAc                                            >  2:706607/1‑140 (MQ=255)                                                                                                 ggCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAcgccgc                                       <  2:926291/140‑1 (MQ=255)                                                                                                 ggCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAcgccgc                                       >  2:1254966/1‑140 (MQ=255)                                                                                                 ggCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAcgccgc                                       >  1:1329426/1‑140 (MQ=255)                                                                                                      ttGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGAt                                  >  2:1280536/1‑140 (MQ=255)                                                                                                      ttGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGAt                                  >  1:206392/1‑140 (MQ=255)                                                                                                        ggTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGt                                >  2:1434602/1‑140 (MQ=255)                                                                                                          tgtCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAcgccg                                        <  1:762741/130‑1 (MQ=255)                                                                                                          tgtCTCCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAcgccg                                        >  2:762741/1‑130 (MQ=255)                                                                                                             ctcCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAc                                            <  1:1179974/123‑1 (MQ=255)                                                                                                             ctcCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGAc                                            >  2:1179974/1‑123 (MQ=255)                                                                                                             ctcCTTTGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGtcttct                           <  1:908613/140‑1 (MQ=255)                                                                                                                   tGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCaaa                     >  2:810320/1‑140 (MQ=255)                                                                                                                   tGAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCaaa                     <  1:1434602/140‑1 (MQ=255)                                                                                                                    gAAGTGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAAc                    >  1:591555/1‑140 (MQ=255)                                                                                                                     aaGTGCCGGTAGCAATTGTGCCGCTGTGCTTGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACg                   <  1:288766/140‑1 (MQ=255)                                                                                                                        tGCCGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTg                <  1:810320/140‑1 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              >  1:1344755/1‑140 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              >  1:936621/1‑140 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              >  2:391431/1‑140 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              >  2:1251930/1‑140 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              >  2:1152562/1‑140 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              <  1:923364/140‑1 (MQ=255)                                                                                                                          ccGGTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGtt              >  1:99705/1‑140 (MQ=255)                                                                                                                            ggTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTgg            <  1:1254966/140‑1 (MQ=255)                                                                                                                            ggTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTgg            <  1:1223815/140‑1 (MQ=255)                                                                                                                             gTAGCAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGc           <  2:591555/140‑1 (MQ=255)                                                                                                                                 cAATTGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATc       <  1:706607/140‑1 (MQ=255)                                                                                                                                    ttGTGCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCg    <  1:391431/140‑1 (MQ=255)                                                                                                                                      gtgCCGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGAt  <  1:1152562/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GCTGGCATTTGGCTTCCTTGCCAATACCGCGCCGGAAGGGGTGCAGGTATCCACCGACATCGCCAGCTATTTAAGCTTCGTTATGGCGCTGTTTATGGCGTTTGGTGTCTCCTTTGAAGTGCCGGTAGCAATTGTGCTGCTGTGCTGGATGGGGATTACCTCGCCAGAAGACTTACGCAAAAAACGCCCGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGAT  >  NC_000913/4023128‑4023400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑