Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,200,096	A→G	intergenic (+2/‑185)	yjaG → / → hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,200,096	0	A	G	100.0%	241.1 / NA	72	intergenic (+2/‑185)	yjaG/hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/33);  total (39/33)

AGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTC  >  NC_000913/4199959‑4200231                                                                                                                                          |                                                                                                                                        aGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGcc                                                                                                                                       >  2:476828/1‑140 (MQ=255)    aTGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGa                                                                                                                                    >  1:598745/1‑140 (MQ=255)    aTGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGa                                                                                                                                    >  2:839239/1‑140 (MQ=255)    aTGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGa                                                                                                                                    >  2:1246799/1‑140 (MQ=255)        gACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAAc                                                                                                                                <  1:405195/140‑1 (MQ=255)            ttCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGa                                                                                                                            >  1:268272/1‑140 (MQ=255)             tCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGAt                                                                                                                           >  2:1072058/1‑140 (MQ=255)             tCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGAt                                                                                                                           >  1:38586/1‑140 (MQ=255)               aGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGAttt                                                                                                                         <  1:890762/140‑1 (MQ=255)               aGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGAttt                                                                                                                         <  1:1246799/140‑1 (MQ=255)                 tGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTaa                                                                                                                       <  1:1047748/140‑1 (MQ=255)                 tGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTaa                                                                                                                       >  2:1479639/1‑140 (MQ=255)                        tatTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGa                                                                                                                <  2:268272/140‑1 (MQ=255)                         atTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGAt                                                                                                               >  2:857247/1‑140 (MQ=255)                         atTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGAt                                                                                                               <  2:598745/140‑1 (MQ=255)                             cGACTTTTAGCTGAGTGCGAAGAACGCGATACCGAGCTGACAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGt                                                                                                           <  1:1414719/140‑1 (MQ=255)                              gACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTc                                                                                                          <  2:1215540/140‑1 (MQ=255)                               aCTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCg                                                                                                         <  1:754882/140‑1 (MQ=255)                                  tttAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTAc                                                                                                      <  1:1400259/140‑1 (MQ=255)                                    tAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCt                                                                                                    <  1:1318783/140‑1 (MQ=255)                                       cTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGa                                                                                                 <  1:476828/140‑1 (MQ=255)                                           gTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCt                                                                                             >  1:125527/1‑140 (MQ=255)                                           gTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCt                                                                                             >  1:1126843/1‑140 (MQ=255)                                                    aCGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCg                                                                                    >  2:508303/1‑140 (MQ=255)                                                          tatCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGccc                                                                                 <  2:1297858/137‑1 (MQ=255)                                                          tatCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGccc                                                                                 >  1:1297858/1‑137 (MQ=255)                                                             cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAATGCCTGATCTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                                                           >  2:1034395/1‑140 (MQ=255)                                                             cGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                                                           <  2:38586/140‑1 (MQ=255)                                                                gCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCAGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTgg                                                                        >  2:690920/1‑140 (MQ=255)                                                                  tGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTc                                                                      <  2:787284/140‑1 (MQ=255)                                                                    aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTa                                                                    <  2:947421/140‑1 (MQ=255)                                                                    aTAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAACAATAAGGCCAGAAAACGCGATTTAACGCCTGCTTTGTCGTACCTGGAGCCTTCCCTTTCGCCCCCCGTCTGGTCTa                                                                    >  1:261549/1‑140 (MQ=255)                                                                              ttAGGGCAGACCTGCGTGAGGCGGGTGCGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                                                           >  2:49652/1‑123 (MQ=255)                                                                              ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTc                                                                           <  1:49652/123‑1 (MQ=255)                                                                              ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTa                                                                    >  2:36526/1‑130 (MQ=255)                                                                              ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTa                                                                    <  1:36526/130‑1 (MQ=255)                                                                              ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTggggg                                                          >  2:1013785/1‑140 (MQ=255)                                                                                  ggCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCAACATTTGGGGGGCGa                                                      >  2:540102/1‑140 (MQ=255)                                                                                     aGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTggg                                                            <  1:1193182/131‑1 (MQ=255)                                                                                     aGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTggg                                                            >  2:1193182/1‑131 (MQ=255)                                                                                     aGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaa                                                   >  1:543407/1‑140 (MQ=255)                                                                                       aCCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaa                                                 >  2:337764/1‑140 (MQ=255)                                                                                       aCCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaa                                                 >  1:763655/1‑140 (MQ=255)                                                                                        ccTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGaaaaaaa                                                <  1:839239/140‑1 (MQ=255)                                                                                            cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg                                            >  1:31922/1‑140 (MQ=255)                                                                                            cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg                                            >  1:1240377/1‑140 (MQ=255)                                                                                            cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg                                            >  2:623946/1‑140 (MQ=255)                                                                                                 ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg                                            <  1:1379798/135‑1 (MQ=255)                                                                                                 ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTgg                                            >  2:1379798/1‑135 (MQ=255)                                                                                                 ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATc                                       >  2:1146435/1‑140 (MQ=255)                                                                                                 ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATc                                       <  2:1240377/140‑1 (MQ=255)                                                                                                 ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATc                                       >  2:330552/1‑140 (MQ=255)                                                                                                  gCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCg                                      <  1:1072058/140‑1 (MQ=255)                                                                                                   cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCgg                                     <  2:31922/140‑1 (MQ=255)                                                                                                   cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCgg                                     <  1:623946/140‑1 (MQ=255)                                                                                                   cGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCgg                                     <  1:508303/140‑1 (MQ=255)                                                                                                     ggTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTg                                   >  1:1175904/1‑140 (MQ=255)                                                                                                      gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAATGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGc                                  <  1:1034395/140‑1 (MQ=255)                                                                                                      gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGc                                  <  2:1126843/140‑1 (MQ=255)                                                                                                      gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCAACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGc                                  <  1:540102/140‑1 (MQ=255)                                                                                                        gagaGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCgt                                <  2:543407/140‑1 (MQ=255)                                                                                                        gagaGCAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCgt                                <  2:125527/140‑1 (MQ=255)                                                                                                             cAATATTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATg                           >  2:945725/1‑140 (MQ=255)                                                                                                                tatTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAg                        >  2:1119467/1‑140 (MQ=255)                                                                                                                tatTGGTATAATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAg                        <  1:330552/140‑1 (MQ=255)                                                                                                                        taATTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGc                >  1:872615/1‑140 (MQ=255)                                                                                                                          aTTTTTCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTa              >  2:877217/1‑140 (MQ=255)                                                                                                                           tttttCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTAt             <  1:857247/140‑1 (MQ=255)                                                                                                                               tCAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTg         >  2:946874/1‑140 (MQ=255)                                                                                                                                cAGCAATAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTgg        >  1:590181/1‑140 (MQ=255)                                                                                                                                      tAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTc  >  2:436460/1‑140 (MQ=255)                                                                                                                                      tAAGGCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTc  >  1:1118913/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AGAATGGGACATTCAGTGGGAAATATTCCGACTTTTAGCTGAGTGCGAAGAACGCGATATCGAGCTGATAAAAGGCCTTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGGGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTC  >  NC_000913/4199959‑4200231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑