Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	746	T→C	V137A (GTA→GCA)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	746	0	T	C	100.0%	206.6 / NA	60	V137A (GTA→GCA)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/30);  total (30/30)

CGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGC  >  NC_000913/615‑878                                                                                                                                    |                                                                                                                                     cgtcgATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAg                                                                                                                              <  2:27315/140‑1 (MQ=255)         aGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGt                                                                                                                      <  1:104020/140‑1 (MQ=255)           gAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTca                                                                                                                    <  1:950366/140‑1 (MQ=255)           gAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGCCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTca                                                                                                                    <  1:1387014/140‑1 (MQ=255)                tGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACg                                                                                                               >  2:22664/1‑140 (MQ=255)                tGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACg                                                                                                               >  1:517468/1‑140 (MQ=255)                 gCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGt                                                                                                              <  1:650313/140‑1 (MQ=255)                  cccAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGtt                                                                                                             >  1:992889/1‑140 (MQ=255)                  cccAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGtt                                                                                                             >  1:374479/1‑140 (MQ=255)                    cAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTAc                                                                                                           <  1:1394035/140‑1 (MQ=255)                    cAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTAc                                                                                                           <  2:1255725/140‑1 (MQ=255)                      aaTAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTg                                                                                                         >  2:1335350/1‑140 (MQ=255)                               tGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATc                                                                                                <  1:419788/140‑1 (MQ=255)                                gTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATcc                                                                                               <  2:797155/140‑1 (MQ=255)                                 tCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTAc                                                                                                           >  2:150267/1‑127 (MQ=255)                                 tCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTAc                                                                                                           <  1:150267/127‑1 (MQ=255)                                 tCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGAt                                                                                                 <  1:768663/137‑1 (MQ=255)                                 tCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGAt                                                                                                 >  2:768663/1‑137 (MQ=255)                                   cTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGt                                                                                            >  2:674370/1‑140 (MQ=255)                                     gCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCg                                                                                          >  2:872509/1‑140 (MQ=255)                                      cATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGa                                                                                         >  1:972904/1‑140 (MQ=255)                                      cATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGa                                                                                         >  2:248691/1‑140 (MQ=255)                                           cATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAAc                                                                                    >  2:195569/1‑140 (MQ=255)                                           cATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAAc                                                                                    >  2:1326080/1‑140 (MQ=255)                                           cATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAAc                                                                                    >  2:102524/1‑140 (MQ=255)                                           cATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGT‑GCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAAct                                                                                   >  2:662475/1‑140 (MQ=255)                                              tAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAActgc                                                                                 <  1:730412/140‑1 (MQ=255)                                              tAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAActgc                                                                                 >  2:1119035/1‑140 (MQ=255)                                               aGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAActgct                                                                                <  1:674370/140‑1 (MQ=255)                                                  ttgttgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGTCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGc                                                                             <  2:453561/140‑1 (MQ=255)                                                    gttgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAg                                                                           <  1:1335350/140‑1 (MQ=255)                                                     ttgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGt                                                                          <  1:667590/140‑1 (MQ=255)                                                     ttgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGt                                                                          >  2:53488/1‑140 (MQ=255)                                                     ttgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGt                                                                          >  2:606333/1‑140 (MQ=255)                                                     ttgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGt                                                                          >  1:950704/1‑140 (MQ=255)                                                      tgGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTg                                                                         <  1:102524/140‑1 (MQ=255)                                                           cAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCa                                                                    <  1:195569/140‑1 (MQ=255)                                                           cAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCa                                                                    <  1:248691/140‑1 (MQ=255)                                                            aGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCAt                                                                   <  2:374479/140‑1 (MQ=255)                                                                      tAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAAt                                                         <  2:992889/140‑1 (MQ=255)                                                                      tAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAAt                                                         <  1:1326080/140‑1 (MQ=255)                                                                      tAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAAt                                                         <  1:189482/140‑1 (MQ=255)                                                                        gCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCt                                                       <  2:517468/140‑1 (MQ=255)                                                                         cATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTa                                                      >  1:1096178/1‑140 (MQ=255)                                                                         cATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTa                                                      >  1:697169/1‑140 (MQ=255)                                                                         cATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTa                                                      >  1:743103/1‑140 (MQ=255)                                                                          aTCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTAc                                                     >  2:379228/1‑140 (MQ=255)                                                                           tCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTAcc                                                    <  2:743103/140‑1 (MQ=255)                                                                             aaCGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGt                                                  <  2:56780/140‑1 (MQ=255)                                                                              aCGCTGCGCTGATTTGCCGT‑GCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCg                                                <  1:662475/140‑1 (MQ=255)                                                                               cgctgcgctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCg                                                <  1:379228/140‑1 (MQ=255)                                                                                  tgcgctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGata                                             >  1:756682/1‑140 (MQ=255)                                                                                     gctgATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTg                                          <  1:606333/140‑1 (MQ=255)                                                                                           ttGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGt                                    <  1:53488/140‑1 (MQ=255)                                                                                                   gCGAGAAAATGTCGATCGCCATTGTGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACccgc                            >  1:913461/1‑140 (MQ=255)                                                                                                   gCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACccgc                            >  1:1197533/1‑140 (MQ=255)                                                                                                   gCGAGAAAATGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACccgc                            >  1:966788/1‑140 (MQ=255)                                                                                                        aaaaTGTCGATCGCCATTATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTAt                       >  1:1001459/1‑140 (MQ=255)                                                                                                                        ttATGGCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCAtt       >  1:1107837/1‑140 (MQ=255)                                                                                                                            ggCCGGCGCATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCgg   >  2:1404376/1‑140 (MQ=255)                                                                                                                             gcgggCGAATAAGAGGCGCGGGGTCAAAACTTTATTTTTATCGCCCCGGTAAAAAACCGGCGGGCGGGGGGGCATCCCCCCAAATCTCCCGTCGATTTTGCGGAGCCCCCCCGCCGTATTGCGGCAAGCCGCATTCCGGc  <  2:756682/137‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     CGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGC  >  NC_000913/615‑878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑