Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,202,187	C→T	P88S (CCG→TCG)	zraS →	sensory histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,202,187	0	C	T	100.0%	245.5 / NA	70	P88S (CCG→TCG)	zraS	sensory histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/36);  total (34/36)

CGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTAAAGCCAGAGGAAAACTCCCG  >  NC_000913/4202051‑4202317                                                                                                                                         |                                                                                                                                   cGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCgg                                                                                                                                 <  1:878051/140‑1 (MQ=255) cGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCgg                                                                                                                                 <  1:401122/140‑1 (MQ=255)   aGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGa                                                                                                                               <  2:1434066/140‑1 (MQ=255)   aGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGa                                                                                                                               <  2:1471863/140‑1 (MQ=255)   aGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGa                                                                                                                               <  2:738282/140‑1 (MQ=255)     gCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAgt                                                                                                                             >  2:120571/1‑140 (MQ=255)     gCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAgt                                                                                                                             >  2:273736/1‑140 (MQ=255)             ccAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGt                                                                                                                     >  2:596978/1‑140 (MQ=255)              cAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGCGTTGTgggc                                                                                                                    >  2:1094509/1‑138 (MQ=255)               aGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTc                                                                                                                   <  2:1075935/140‑1 (MQ=255)                    ttACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGt                                                                                                              <  2:897004/140‑1 (MQ=255)                    ttACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGt                                                                                                              <  2:1047670/140‑1 (MQ=255)                    ttACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGt                                                                                                              >  1:476760/1‑140 (MQ=255)                    ttACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGt                                                                                                              <  1:1094509/140‑1 (MQ=255)                       cTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCAc                                                                                                           <  1:1365343/140‑1 (MQ=255)                           aaaaaGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGtgttg                                                                                                                         >  1:1272287/1‑122 (MQ=255)                           aaaaaGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGtgttg                                                                                                                         <  2:1272287/122‑1 (MQ=255)                            aaaaGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTc                                                                                                                   <  2:355348/127‑1 (MQ=255)                            aaaaGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTc                                                                                                                   >  1:355348/1‑127 (MQ=255)                              aaGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATgcg                                                                                                    <  1:1082357/140‑1 (MQ=255)                              aaGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATgcg                                                                                                    <  1:82975/140‑1 (MQ=255)                               aGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATgcgc                                                                                                   >  1:1282499/1‑140 (MQ=255)                                ggTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCa                                                                                                  <  1:105714/140‑1 (MQ=255)                                   actgtgCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAggg                                                                                               <  2:1002730/138‑1 (MQ=255)                                     tgtgCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGCGTTGTGGTTCGCAGTCACCGATGCGCAGGGcc                                                                                             >  2:1418381/1‑139 (MQ=255)                                      gtgCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGcat                                                                                            <  1:427227/140‑1 (MQ=255)                                       tgCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCg                                                                                                         <  1:712943/126‑1 (MQ=255)                                       tgCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCg                                                                                                         >  2:712943/1‑126 (MQ=255)                                       tgCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGcatc                                                                                           >  2:1332732/1‑140 (MQ=255)                                         cTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGcatcat                                                                                         >  1:599710/1‑140 (MQ=255)                                          ttATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCatt                                                                                        <  1:118168/140‑1 (MQ=255)                                           tATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCatta                                                                                       <  2:1282499/140‑1 (MQ=255)                                            aTCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCattat                                                                                      <  2:599710/140‑1 (MQ=255)                                              ccGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTAttc                                                                                    >  2:1012355/1‑140 (MQ=255)                                                    tctGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATa                                                                              <  1:764172/140‑1 (MQ=255)                                                      tGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGc                                                                            >  2:672945/1‑140 (MQ=255)                                                        gAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGa                                                                          >  2:897567/1‑140 (MQ=255)                                                         aGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGAc                                                                         <  2:380023/140‑1 (MQ=255)                                                         aGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGAc                                                                         >  2:1473532/1‑140 (MQ=255)                                                         aGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGAc                                                                         <  1:443394/140‑1 (MQ=255)                                                               gAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGAt                                                                   <  1:897567/140‑1 (MQ=255)                                                               gAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGAt                                                                   <  1:672945/140‑1 (MQ=255)                                                                  gCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAg                                                                >  1:637310/1‑140 (MQ=255)                                                                  gCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAg                                                                >  1:1474421/1‑140 (MQ=255)                                                                      cgTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCg                                                            <  2:1474421/140‑1 (MQ=255)                                                                             atggggatgCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGAt                                                                   <  2:1468047/126‑1 (MQ=255)                                                                             atggggatgCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTgc                                                     >  1:48067/1‑140 (MQ=255)                                                                             atggggatgCGAATGCACCATGTACAGCAACAGGCACTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGAt                                                                   >  1:1468047/1‑126 (MQ=255)                                                                                gggatgCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGct                                                  >  1:188153/1‑140 (MQ=255)                                                                                      cGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTc                                            <  1:120571/140‑1 (MQ=255)                                                                                      cGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTc                                            <  1:273736/140‑1 (MQ=255)                                                                                                 tGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGaaa                                 >  2:87277/1‑140 (MQ=255)                                                                                                     cAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCa                             >  1:659924/1‑140 (MQ=255)                                                                                                      aGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAg                            <  2:1329473/140‑1 (MQ=255)                                                                                                        cAACAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGaa                          >  1:997739/1‑140 (MQ=255)                                                                                                         aaCAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCa                             <  1:856554/136‑1 (MQ=255)                                                                                                         aaCAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCa                             >  2:856554/1‑136 (MQ=255)                                                                                                           cAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAAtt                       >  2:591691/1‑140 (MQ=255)                                                                                                           cAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAAtt                       >  2:680366/1‑140 (MQ=255)                                                                                                           cAGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAAtt                       <  1:596978/140‑1 (MQ=255)                                                                                                           cAGGCGCTTCTGGAAGAGATGGCGGGACAGTAGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGc                              <  2:507049/133‑1 (MQ=255)                                                                                                           cAGGCGCTTCTGGAAGAGATGGCGGGACAGTAGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGc                              >  1:507049/1‑133 (MQ=255)                                                                                                            aGGCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTa                      >  2:1229112/1‑140 (MQ=255)                                                                                                             ggCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTaa                     >  2:533524/1‑140 (MQ=255)                                                                                                             ggCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTaa                     >  2:1168495/1‑140 (MQ=255)                                                                                                             ggCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTaa                     >  1:1436279/1‑140 (MQ=255)                                                                                                             ggCGCTTCTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTaa                     >  2:1362966/1‑140 (MQ=255)                                                                                                                    cTGGAAGAGATGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTAAAGCCaga              <  1:680366/140‑1 (MQ=255)                                                                                                                          gagaTGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTAAAGCCAGAGGaaaa        >  2:781917/1‑140 (MQ=255)                                                                                                                              tGGCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTAAAGCCAGAGGAAAACTcc    >  1:423665/1‑140 (MQ=255)                                                                                                                                gCGGGACAGTCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTAAAGCCAGAGGAAAACTCCCg  <  2:476760/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGTTCGCAGTCACCGATGCGCAGGGCATCATTATTCTTCATAGCGACCCCGATAAGGTCGGGCGTGCGCTCTATTCGCCGGATGAAATGCAGAAATTAAAGCCAGAGGAAAACTCCCG  >  NC_000913/4202051‑4202317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑