Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,165	A→G	Y232C (TAT→TGT)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,165	0	A	G	100.0%	217.4 / NA	65	Y232C (TAT→TGT)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/32);  total (33/32)

TTAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACG  >  NC_000913/4276029‑4276300                                                                                                                                         |                                                                                                                                        ttAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCa                                                                                                                                      <  2:284036/140‑1 (MQ=255) ttAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATCTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCa                                                                                                                                      >  2:976754/1‑140 (MQ=255)   aaGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAAc                                                                                                                                    <  1:1399267/140‑1 (MQ=255)    aGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACg                                                                                                                                   <  1:976754/140‑1 (MQ=255)              ccTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACg                                                                                                                                   >  1:1209593/1‑130 (MQ=255)              ccTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACg                                                                                                                                   <  2:1209593/130‑1 (MQ=255)              ccTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAAcc                                                                                                                         <  2:362090/140‑1 (MQ=255)              ccTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAAcc                                                                                                                         >  2:1164513/1‑140 (MQ=255)              ccTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAAcc                                                                                                                         <  1:448779/140‑1 (MQ=255)              ccTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAAcc                                                                                                                         >  1:259922/1‑140 (MQ=255)                tCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCa                                                                                                                       <  1:1140980/140‑1 (MQ=255)                tCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCa                                                                                                                       <  2:463460/140‑1 (MQ=255)                tCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCa                                                                                                                       >  1:491549/1‑140 (MQ=255)                 cGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAAcc                                                                                                                         <  2:1367752/137‑1 (MQ=255)                 cGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAAcc                                                                                                                         >  1:1367752/1‑137 (MQ=255)                        aaCGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTtata                                                                                                               <  1:1133342/140‑1 (MQ=255)                             tttCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAatcat                                                                                                          <  2:259922/140‑1 (MQ=255)                                 tcCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTa                                                                                                      >  2:998650/1‑140 (MQ=255)                                             tttGAAGGATTGAAGCGGCCAAGGAAATGGCGATTTATATGCGACAGTTTATTCGTCAAACCGCCCAATTGCAGCCATTGTTGCGACTTCATGACAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGc                                                                                          >  2:377952/1‑140 (MQ=255)                                               tGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGccc                                                                                        >  2:526555/1‑140 (MQ=255)                                                   ggATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgccg                                                                                    <  1:638471/140‑1 (MQ=255)                                                             ttACAAAGAAATGGTTATTTATATGCGACAGTTTATTCGAAAAAACCCCCATTTGCAGCCATTGTTGCGATTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTggg                                                                          <  2:61293/139‑1 (MQ=255)                                                             gTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTggg                                                                          <  1:515770/140‑1 (MQ=255)                                                             gTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTggg                                                                          <  1:526555/140‑1 (MQ=255)                                                             gTAAAAAGAAATGGCTATTTATATGCGACAGTTTATCCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTTTTATAACCCATTTTTATAATCATCTTATTTTTGCGTGGCCCGCCGGTATTTTggg                                                                          <  2:1014651/140‑1 (MQ=255)                                                              tACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTgggg                                                                         >  2:978110/1‑140 (MQ=255)                                                              tACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTgggg                                                                         >  2:821262/1‑140 (MQ=255)                                                               aCAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGa                                                                        >  1:61293/1‑140 (MQ=255)                                                                cAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgccg                                                                                    >  2:499113/1‑127 (MQ=255)                                                                cAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgccg                                                                                    <  1:499113/127‑1 (MQ=255)                                                                cAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgc                                                                                      >  2:472526/1‑125 (MQ=255)                                                                cAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgc                                                                                      <  1:472526/125‑1 (MQ=255)                                                                   aGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgccg                                                                                    <  2:184592/124‑1 (MQ=255)                                                                   aGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCccgccg                                                                                    >  1:184592/1‑124 (MQ=255)                                                                        tGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTc                                                               >  2:344883/1‑140 (MQ=255)                                                                        tGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTc                                                               <  1:1180141/140‑1 (MQ=255)                                                                        tGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTc                                                               >  1:144095/1‑140 (MQ=255)                                                                        tGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTc                                                               >  2:939266/1‑140 (MQ=255)                                                                        tGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTc                                                               >  1:1354773/1‑140 (MQ=255)                                                                          gCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTg                                                             <  1:998650/140‑1 (MQ=255)                                                                          gCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTg                                                             >  1:606974/1‑140 (MQ=255)                                                                            tATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCt                                                           <  2:491549/140‑1 (MQ=255)                                                                               ttATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGt                                                                 >  2:937361/1‑131 (MQ=255)                                                                               ttATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGt                                                                 <  1:937361/131‑1 (MQ=255)                                                                               ttATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTAtt                                                        >  2:1185761/1‑140 (MQ=255)                                                                                  tatGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTAct                                                     <  1:344883/140‑1 (MQ=255)                                                                                  tatGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTAct                                                     <  1:978110/140‑1 (MQ=255)                                                                                  tatGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTAct                                                     <  1:1164513/140‑1 (MQ=255)                                                                                   atGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTActc                                                    <  1:1149465/140‑1 (MQ=255)                                                                                        aCAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTAt                                                         <  1:621266/130‑1 (MQ=255)                                                                                        aCAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTAt                                                         >  2:621266/1‑130 (MQ=255)                                                                                        aCAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCt                                               >  1:135433/1‑140 (MQ=255)                                                                                        aCAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCt                                               >  1:1018242/1‑140 (MQ=255)                                                                                        aCAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCt                                               >  2:88461/1‑140 (MQ=255)                                                                                         cAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTa                                              <  2:135433/140‑1 (MQ=255)                                                                                          aGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTAc                                             >  1:911244/1‑140 (MQ=255)                                                                                                    aCAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACa                                   <  2:1434636/140‑1 (MQ=255)                                                                                                     cAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACaa                                  <  2:1018242/140‑1 (MQ=255)                                                                                                      aaaaCGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACaaa                                 >  1:1396645/1‑140 (MQ=255)                                                                                                          cGCCCAATTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGAATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTa                             >  2:423338/1‑140 (MQ=255)                                                                                                               aaTTGCAGCCATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTAt                        >  2:457858/1‑140 (MQ=255)                                                                                                                       ccATTGTTGCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCaaa                >  1:886946/1‑140 (MQ=255)                                                                                                                           tgttgCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTa            >  1:874869/1‑140 (MQ=255)                                                                                                                              tgCGACTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAAt         >  1:370713/1‑140 (MQ=255)                                                                                                                                   cTTCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCaa    >  1:1213977/1‑140 (MQ=255)                                                                                                                                     tCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAAcg  <  2:171476/140‑1 (MQ=255)                                                                                                                                     tCATGTCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAAcg  <  1:939266/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TTAAGCAAAGAGGCCTCGGCAGCAACGTTTTCTCCGCTGATTCATTTGAAGGATTTAACCGTACAAAGAAATGGCTATTTATATGCGACAGTTTATTCGACAAAACGCCCAATTGCAGCCATTGTTGCGACTTCATATCAACGTCTTATAACCCATTTTTATAATCATCTTATTTTTGCGTTGCCCGCCGGTATTTTGGGGAGTCTTGTTCTGCTATTACTCTGGCTACGTATTCGACAAAACTATTTATCTCCCAAACGTAAATTGCAACG  >  NC_000913/4276029‑4276300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑