Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,716	A→G	V219A (GTA→GCA)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,716	0	A	G	100.0%	222.4 / NA	65	V219A (GTA→GCA)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/28);  total (37/28)

TAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAGTTGG  >  NC_000913/4286584‑4286839                                                                                                                                     |                                                                                                                            tAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCcaccac                                                                                                                      >  1:1294876/1‑140 (MQ=255) aagaaAAAAAACAGCGGATCTTCGGCTTTCTTCTCTCCCGCCTGGTGCTGATCGCTCGCTTGCCCAACCAGGTCGCCCCCCCACGGGCCGCCCCCTCCCTGCCATTCATTTTTCCCGCCAGTACGCTTCAGTGCcaccac                                                                                                                      <  2:634558/139‑1 (MQ=255)  agagAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                                                     >  1:1321083/1‑140 (MQ=255)  agaaAAAAACCAGCGGTTCTTCGGCTTTCTTCTCTCCCGCTTGGGGCTGCCCGCTCGCTTGCCCACCCGGGTCGCCCCCCCACGGGCCGCCCCCTTCCTGCCAGTAATTTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                                                     <  2:858843/140‑1 (MQ=255)        tAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                                                               >  1:634558/1‑140 (MQ=255)         aaaCAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTa                                                                                                              >  2:351255/1‑140 (MQ=255)           aCAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACg                                                                                                            >  2:10518/1‑140 (MQ=255)             aGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCt                                                                                                          >  2:1121986/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  1:611515/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  1:480640/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  2:107325/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  1:227249/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  2:163917/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  2:359615/1‑140 (MQ=255)                ggATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                                                       >  2:553584/1‑140 (MQ=255)                  aTCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGa                                                                                                     >  2:674239/1‑140 (MQ=255)                    cTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACg                                                                                                   >  1:6167/1‑140 (MQ=255)                     ctCGCCGTTCTTCTCTCCCCCCTGGTGGTGACCGCCCACTTGCCCACCCAGGCCGCCCCCCCACAGCCCGCCCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGt                                                                                                  <  2:56972/139‑1 (MQ=255)                            ttCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                                                           >  1:1029401/1‑140 (MQ=255)                            ttCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                                                           >  1:755921/1‑140 (MQ=255)                                    tcccGCCTGGTGCTGACCGCCCGCTTGCCCACCCGGGCCGCCCCCCCACATGTCGCCCCCCTCCTCCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAgcg                                                                                   <  2:1321083/138‑1 (MQ=255)                                       cGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGt                                                                                >  1:941093/1‑140 (MQ=255)                                              tGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                                                         >  1:304078/1‑140 (MQ=255)                                                   aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                                                    >  2:1330379/1‑140 (MQ=255)                                                   aTCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTAAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtttt                                                                    >  2:1015662/1‑140 (MQ=255)                                                       ctcgttTGCTCACCCAGGTCGCCCCCCCACAGGCCGCCCCCTTCCTCCCAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                                                                <  1:1403751/140‑1 (MQ=255)                                                       ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                                                                >  1:1031822/1‑140 (MQ=255)                                                       ctcgctTGCTCAACCAGGTCGTCCCCCCACAGGTCGCCCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                                                                <  1:1157794/140‑1 (MQ=255)                                                        tcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                                                               >  1:1329398/1‑140 (MQ=255)                                                             tGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                                                          >  1:359392/1‑140 (MQ=255)                                                                        gTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc                                               >  1:297446/1‑140 (MQ=255)                                                                        gTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAACTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGCGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc                                               >  2:1188025/1‑140 (MQ=255)                                                                          cgcccccccacGGGTCGCCCCCTTCCTCCAAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg                                             <  2:755921/133‑1 (MQ=255)                                                                             gCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgca                                          >  1:696727/1‑140 (MQ=255)                                                                                     gggccgccCCCTCCCTCCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACAcc                                  <  2:6167/136‑1 (MQ=255)                                                                                      ggTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCt                                 >  2:551193/1‑140 (MQ=255)                                                                                       gTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCtt                                >  2:350857/1‑140 (MQ=255)                                                                                          gcgcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTtcgt                             >  2:769159/1‑140 (MQ=255)                                                                                           cgcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATATTGCGCCCGGCACGCACACCTtcgtc                            >  1:1439097/1‑140 (MQ=255)                                                                                           cgcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTtcgtc                            >  1:98721/1‑140 (MQ=255)                                                                                             ccctccctGCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCgg                          <  2:696727/140‑1 (MQ=255)                                                                                             ccctccctCCCCTTCAATTCCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCgg                          <  2:1294876/140‑1 (MQ=255)                                                                                             cccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCgg                          <  2:941093/140‑1 (MQ=255)                                                                                               cTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGaa                        <  1:350857/140‑1 (MQ=255)                                                                                                ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAg                       <  1:351255/140‑1 (MQ=255)                                                                                                ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAg                       <  2:480640/140‑1 (MQ=255)                                                                                                       cattcttttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCAc                <  2:156231/133‑1 (MQ=255)                                                                                                       caataattttcCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCAc                <  1:119491/133‑1 (MQ=255)                                                                                                        aGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCAcc               >  1:698831/1‑140 (MQ=255)                                                                                                          tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCAc                >  1:606251/1‑137 (MQ=255)                                                                                                          tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCAc                <  2:606251/137‑1 (MQ=255)                                                                                                          tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAg             >  2:498068/1‑140 (MQ=255)                                                                                                          tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAg             >  1:667361/1‑140 (MQ=255)                                                                                                          tCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAg             >  2:141480/1‑140 (MQ=255)                                                                                                             tttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCg          <  2:1031822/139‑1 (MQ=255)                                                                                                             aTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCg          <  1:498068/140‑1 (MQ=255)                                                                                                              ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGt         <  2:1029401/140‑1 (MQ=255)                                                                                                              ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGt         <  2:1197404/140‑1 (MQ=255)                                                                                                              ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGt         <  1:674239/140‑1 (MQ=255)                                                                                                                 tCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAg      <  2:667361/140‑1 (MQ=255)                                                                                                                 tCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAg      <  2:359392/140‑1 (MQ=255)                                                                                                                   ccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAGtt    <  1:551193/140‑1 (MQ=255)                                                                                                                   ccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAGtt    <  1:107325/140‑1 (MQ=255)                                                                                                                     gCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAGTTgg  <  1:141480/140‑1 (MQ=255)                                                                                                                     gCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAGTTgg  <  2:98721/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                            TAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACACCTTCGTCGGAAGTGATCACCAGTCGTGAGTTGG  >  NC_000913/4286584‑4286839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑