Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,339,091	T→C	K477K (AAA→AAG)	adiA ←	arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,091	0	T	C	100.0%	273.3 / NA	79	K477K (AAA→AAG)	adiA	arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/41);  total (38/41)

CTTTGAAGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGTTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGT  >  NC_000913/4338955‑4339227                                                                                                                                         |                                                                                                                                         cTTTGAAGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTg                                                                                                                                       >  2:560561/1‑140 (MQ=255) cTTTGAAGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGAGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTg                                                                                                                                       >  1:1092380/1‑140 (MQ=255)     gAAGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTgaaga                                                                                                                                   <  2:1138663/140‑1 (MQ=255)     gAAGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTgaaga                                                                                                                                   <  1:48232/140‑1 (MQ=255)      aaGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTgaagaa                                                                                                                                  >  1:341941/1‑140 (MQ=255)        gCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTCGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAAcc                                                                                                                                >  2:1330544/1‑140 (MQ=255)          cGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCGCGGCTTGAAGAACCAg                                                                                                                              >  2:1199144/1‑140 (MQ=255)            tGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCGGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCt                                                                                                                            >  1:1351463/1‑140 (MQ=255)            tGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCt                                                                                                                            >  2:1193343/1‑140 (MQ=255)            tGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCt                                                                                                                            >  1:811819/1‑140 (MQ=255)            tGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCt                                                                                                                            >  2:202170/1‑140 (MQ=255)            tGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCt                                                                                                                            >  2:656955/1‑140 (MQ=255)            tGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCt                                                                                                                            >  2:818154/1‑140 (MQ=255)                      cGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCg                                                                                                                  <  2:985780/140‑1 (MQ=255)                       gCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTAAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCgg                                                                                                                 >  1:1200644/1‑140 (MQ=255)                        cccGGATGCATTACCCAGCAGTCCTGACCGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTGGAAGAACCAGCTACCGTCAGCGGt                                                                                                                <  2:872623/140‑1 (MQ=255)                         ccGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTg                                                                                                               <  1:268900/140‑1 (MQ=255)                         ccGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTg                                                                                                               <  2:507105/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCTCGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  2:1346460/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  1:355214/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  1:1259849/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  1:19975/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  2:1265264/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  1:1193343/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTCGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  1:1330544/140‑1 (MQ=255)                          cGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTAAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  2:1200644/140‑1 (MQ=255)                           ggATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGaa                                                                                                             <  1:293637/140‑1 (MQ=255)                             aTGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAAct                                                                                                           <  2:409599/140‑1 (MQ=255)                             aTGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAAct                                                                                                           <  1:1480079/140‑1 (MQ=255)                               gCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAActct                                                                                                         <  2:811819/140‑1 (MQ=255)                                       cagcagTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAg                                                                                                 >  2:159780/1‑140 (MQ=255)                                       cagcagTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAg                                                                                                 >  2:80702/1‑140 (MQ=255)                                         gcagTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              <  2:1444550/125‑1 (MQ=255)                                         gcagTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGa                                                                                                              >  1:1444550/1‑125 (MQ=255)                                          cagTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGt                                                                                                                >  1:1198958/1‑122 (MQ=255)                                          cagTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGt                                                                                                                <  2:1198958/122‑1 (MQ=255)                                           agTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCcgc                                                                                             >  2:813183/1‑140 (MQ=255)                                               cTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAAct                                                                                                           <  1:21427/122‑1 (MQ=255)                                               cTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAAct                                                                                                           >  2:21427/1‑122 (MQ=255)                                               cTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCa                                                                                         >  2:142957/1‑140 (MQ=255)                                               cTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCa                                                                                         <  1:80702/140‑1 (MQ=255)                                               cTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCa                                                                                         >  1:1476035/1‑140 (MQ=255)                                               cTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCa                                                                                         <  1:1332722/140‑1 (MQ=255)                                                       tggtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 >  2:154053/1‑140 (MQ=255)                                                       tggtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 <  2:146791/140‑1 (MQ=255)                                                       tggtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 >  1:1115170/1‑140 (MQ=255)                                                       tggtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 <  1:1342062/140‑1 (MQ=255)                                                       tggtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 >  1:146791/1‑140 (MQ=255)                                                       tggtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 >  1:631598/1‑140 (MQ=255)                                                         gtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 >  1:178165/1‑138 (MQ=255)                                                         gtCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGa                                                                                 <  2:178165/138‑1 (MQ=255)                                                                    ggttggtGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCGCGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCg                                                                    <  1:1199144/140‑1 (MQ=255)                                                                      ttggtGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCt                                                                  <  2:1115170/140‑1 (MQ=255)                                                                      ttggtGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCt                                                                  <  1:520270/140‑1 (MQ=255)                                                                           gCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTc                                                             >  2:714603/1‑140 (MQ=255)                                                                            cGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCa                                                            >  2:773856/1‑140 (MQ=255)                                                                               cAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATc                                                         <  1:813183/140‑1 (MQ=255)                                                                               cAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATc                                                         >  2:456398/1‑140 (MQ=255)                                                                                aGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCa                                                        <  1:1137077/140‑1 (MQ=255)                                                                                    aaGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTc                                                    >  2:1179129/1‑140 (MQ=255)                                                                                      gTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCt                                                  <  2:1476035/140‑1 (MQ=255)                                                                                          tAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTgtgt                                              <  1:208227/140‑1 (MQ=255)                                                                                                        ttGTGGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGc                                >  1:835298/1‑140 (MQ=255)                                                                                                          gtgGGTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTg                              <  1:160668/140‑1 (MQ=255)                                                                                                             ggTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTg                           >  1:1460236/1‑140 (MQ=255)                                                                                                             ggTCGGTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACCTCCTGTGTCAGTGACAGGCCGCTGTTg                           >  2:1476638/1‑140 (MQ=255)                                                                                                                 ggTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGt                       <  1:773856/140‑1 (MQ=255)                                                                                                                 ggTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGt                       <  2:835298/140‑1 (MQ=255)                                                                                                                 ggTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGt                       <  1:795027/140‑1 (MQ=255)                                                                                                                 ggTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGt                       >  1:387629/1‑140 (MQ=255)                                                                                                                 ggTGACGACTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGt                       >  2:512431/1‑140 (MQ=255)                                                                                                                        acTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCcatcat                <  1:818154/140‑1 (MQ=255)                                                                                                                        acTTCTTTGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCcatcat                >  1:954801/1‑140 (MQ=255)                                                                                                                             tttGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGaca           <  2:387629/140‑1 (MQ=255)                                                                                                                             tttGTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGaca           >  1:1319469/1‑140 (MQ=255)                                                                                                                                gTTCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACCTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCg        <  1:1476638/140‑1 (MQ=255)                                                                                                                                 ttCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGc       >  2:896687/1‑140 (MQ=255)                                                                                                                                  tCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGcc      <  1:142957/140‑1 (MQ=255)                                                                                                                                  tCCACGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGcc      <  2:208640/140‑1 (MQ=255)                                                                                                                                      cGGCTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCAcgt  >  1:327672/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CTTTGAAGCCGTGCCAGCTTTCGCCCGGATGCATTACCCAGCAGTCCTGAACGGTGGTCAGCAGTTTGGTTGGTGCGTCAGCAAAGTCATAGGTTTTGCCGGTTTGTGGGTCGGTGACGACTTCTTTGTTCCACGGTTTGAAGAACCAGCTACCGTCAGCGGTGAACTCTTTATATAGCCGCGCCATCGCCTGACGGAAATCAACCGCTTCGTCAATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGT  >  NC_000913/4338955‑4339227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑