Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,669	A→G	T29A (ACC→GCC)	tsaE →	N(6)‑L‑threonylcarbamoyladenine synthase, TsaE subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,669	0	A	G	100.0%	309.2 / NA	88	T29A (ACC→GCC)	tsaE	N(6)‑L‑threonylcarbamoyladenine synthase, TsaE subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/48);  total (40/48)

CTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACT  >  NC_000913/4395533‑4395804                                                                                                                                         |                                                                                                                                        ctctTTTCCACGCTACATCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCTTGATGAGCAGGCAACATTCGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      <  2:1315906/140‑1 (MQ=255) ctctTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      <  1:887291/140‑1 (MQ=255) ctctTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      <  2:705199/140‑1 (MQ=255) ctctTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      <  1:94692/140‑1 (MQ=255) ctctTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      <  2:1256079/140‑1 (MQ=255)  tctTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGt                                                                                                                                     <  1:1462161/140‑1 (MQ=255)    ttttCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTaa                                                                                                                                   <  1:1478700/140‑1 (MQ=255)         cACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAatctat                                                                                                                              <  2:713113/140‑1 (MQ=255)           cGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      >  1:1350642/1‑130 (MQ=255)           cGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCg                                                                                                                                      <  2:1350642/130‑1 (MQ=255)              tACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATAGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTa                                                                                                                         >  2:507761/1‑140 (MQ=255)                cAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAatctat                                                                                                                              >  2:1108824/1‑133 (MQ=255)                cAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATg                                                                                                                       >  1:565443/1‑140 (MQ=255)                cAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAAGCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAatctat                                                                                                                              <  1:1108824/133‑1 (MQ=255)                     tATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGAt                                                                                                                  >  1:58506/1‑140 (MQ=255)                      aTTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGAtt                                                                                                                 >  1:983793/1‑140 (MQ=255)                        tGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTa                                                                                                               >  2:700558/1‑140 (MQ=255)                        tGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTa                                                                                                               >  1:99184/1‑140 (MQ=255)                           tAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGgc                                                                                                            >  1:423602/1‑140 (MQ=255)                           tAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGgc                                                                                                            <  2:672962/140‑1 (MQ=255)                            aaCCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGgcg                                                                                                           <  1:700558/140‑1 (MQ=255)                            aaCCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGgcg                                                                                                           <  2:565443/140‑1 (MQ=255)                             aCCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGgcgc                                                                                                          >  2:728768/1‑140 (MQ=255)                             aCCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGgcgc                                                                                                          >  1:716996/1‑140 (MQ=255)                               ccGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAg                                                                                                        >  1:407760/1‑140 (MQ=255)                               ccGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAg                                                                                                        <  1:1190511/140‑1 (MQ=255)                               ccGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAg                                                                                                        >  2:430757/1‑140 (MQ=255)                                  gAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATgg                                                                                                                      >  1:1075270/1‑123 (MQ=255)                                  gAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATgg                                                                                                                      <  2:1075270/123‑1 (MQ=255)                                  gAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTa                                                                                                     >  1:278538/1‑140 (MQ=255)                                    aGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAAACCTGGGCGAGCGGGGAGCGAAAGCCTGCGATGGCGCCGCCGTAATCTATCTGTATGGCGATTTTGGCGCCGGTaaa                                                                                                   >  2:633760/1‑140 (MQ=255)                                     gTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTaaaa                                                                                                  <  2:883023/140‑1 (MQ=255)                                      tGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAac                                                                                                 <  2:407760/140‑1 (MQ=255)                                      tGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGAGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAac                                                                                                 <  1:130908/140‑1 (MQ=255)                                        aCTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAacca                                                                                               >  2:465275/1‑140 (MQ=255)                                                  aCCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCTGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCg                                                                                     <  2:691078/140‑1 (MQ=255)                                                  aCCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCg                                                                                     >  2:360822/1‑140 (MQ=255)                                                     atgatgAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCgggg                                                                                  <  2:278538/140‑1 (MQ=255)                                                      tgatgaATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGc                                                                                 <  1:633760/140‑1 (MQ=255)                                                      tgatgaATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGc                                                                                 >  2:246586/1‑140 (MQ=255)                                                       gatgaATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCt                                                                                >  2:773759/1‑140 (MQ=255)                                                        atgaATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCtt                                                                               <  2:99184/140‑1 (MQ=255)                                                            aTCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTa                                                                           <  1:773759/140‑1 (MQ=255)                                                            aTCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTa                                                                           <  1:56648/140‑1 (MQ=255)                                                            aTCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTa                                                                           >  1:1391839/1‑140 (MQ=255)                                                             tCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTAc                                                                          <  2:423602/140‑1 (MQ=255)                                                              cGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACa                                                                         <  1:430757/140‑1 (MQ=255)                                                              cGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACa                                                                         <  2:1391839/140‑1 (MQ=255)                                                                     ttCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACa                                                                         <  2:605092/133‑1 (MQ=255)                                                                     ttCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACa                                                                         >  1:605092/1‑133 (MQ=255)                                                                      tCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTgg                                                                 <  1:507761/140‑1 (MQ=255)                                                                        cGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGt                                                               <  1:1287730/140‑1 (MQ=255)                                                                          cTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGtca                                                             <  1:465275/140‑1 (MQ=255)                                                                            cccTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGtcatc                                                           <  2:58506/140‑1 (MQ=255)                                                                             ccTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGtcatca                                                          <  2:339220/140‑1 (MQ=255)                                                                               tgatgaGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAgg                                                        >  1:923701/1‑140 (MQ=255)                                                                                         gCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCaa                                              >  2:1461788/1‑140 (MQ=255)                                                                                         gCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCaa                                              >  1:356469/1‑140 (MQ=255)                                                                                              aTTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGcc                                         >  1:358059/1‑140 (MQ=255)                                                                                              aTTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGcc                                         >  1:751474/1‑140 (MQ=255)                                                                                                   aCCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGc                                          <  1:491943/134‑1 (MQ=255)                                                                                                   aCCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGc                                          >  2:491943/1‑134 (MQ=255)                                                                                                   aCCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACt                                    >  2:424161/1‑140 (MQ=255)                                                                                                       gggCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGccc                                        <  2:640622/132‑1 (MQ=255)                                                                                                       gggCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGccc                                        >  1:640622/1‑132 (MQ=255)                                                                                                       gggCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTtata                                >  2:509517/1‑140 (MQ=255)                                                                                                       gggCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTtata                                >  1:393569/1‑140 (MQ=255)                                                                                                        ggCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATAc                               >  1:628862/1‑140 (MQ=255)                                                                                                          cGAGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGc                             <  2:72135/140‑1 (MQ=255)                                                                                                            aGCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTg                           >  2:15910/1‑140 (MQ=255)                                                                                                             gCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGc                                          <  1:222984/124‑1 (MQ=255)                                                                                                             gCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGc                                          >  2:222984/1‑124 (MQ=255)                                                                                                             gCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTgg                          >  1:979071/1‑140 (MQ=255)                                                                                                             gCGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTgg                          >  1:419539/1‑140 (MQ=255)                                                                                                              cGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGt                         >  2:1259595/1‑140 (MQ=255)                                                                                                              cGGGTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGt                         <  2:628862/140‑1 (MQ=255)                                                                                                               gggTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTc                        <  2:439327/140‑1 (MQ=255)                                                                                                                ggTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCg                       <  1:509517/140‑1 (MQ=255)                                                                                                                 gTAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGa                      <  1:366294/140‑1 (MQ=255)                                                                                                                  tAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTta                                  <  2:3107/127‑1 (MQ=255)                                                                                                                  tAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTta                                  >  1:3107/1‑127 (MQ=255)                                                                                                                  tAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGaa                     <  1:15910/140‑1 (MQ=255)                                                                                                                  tAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGaa                     <  2:983793/140‑1 (MQ=255)                                                                                                                  tAGCGAAAGCCTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATCTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGaa                     <  1:885347/140‑1 (MQ=255)                                                                                                                           ccTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGc                             <  1:1233916/123‑1 (MQ=255)                                                                                                                           ccTGCGATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGc                             >  2:1233916/1‑123 (MQ=255)                                                                                                                                gATGGCGCAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACTCTCGa       <  2:979071/140‑1 (MQ=255)                                                                                                                                     cgcAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACa     >  1:202983/1‑137 (MQ=255)                                                                                                                                     cgcAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACa     <  2:202983/137‑1 (MQ=255)                                                                                                                                     cgcAGCCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACt  >  1:800002/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACT  >  NC_000913/4395533‑4395804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑