Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,914	A→G	E23E (GAA→GAG)	ulaE →	L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,914	0	A	G	100.0%	160.3 / NA	47	E23E (GAA→GAG)	ulaE	L‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/14);  total (33/14)

TTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGG  >  NC_000913/4422779‑4423048                                                                                                                                        |                                                                                                                                       ttCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGcc                                                                                                                                    <  2:1326947/140‑1 (MQ=255)                   aCGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGt                                                                                                                  >  1:1448952/1‑140 (MQ=255)                                    ccATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTc                                                                                                 >  2:341690/1‑140 (MQ=255)                                         gCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAAt                                                                                            >  1:1041719/1‑140 (MQ=255)                                             aaCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCg                                                                                        >  2:351638/1‑140 (MQ=255)                                             aaCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCg                                                                                        >  1:491972/1‑140 (MQ=255)                                               cTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGt                                                                                      >  2:860763/1‑140 (MQ=255)                                                  tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGa                                                                                   >  2:27551/1‑140 (MQ=255)                                                   ggggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGAt                                                                                  >  1:869507/1‑140 (MQ=255)                                                    gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATg                                                                                 >  2:95512/1‑140 (MQ=255)                                                    gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATg                                                                                 >  1:526655/1‑140 (MQ=255)                                                    gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATg                                                                                 >  1:241047/1‑140 (MQ=255)                                                       cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa                                                                              <  2:241047/140‑1 (MQ=255)                                                        tAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAAc                                                                             <  2:869507/140‑1 (MQ=255)                                                         aaGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACt                                                                            >  1:264049/1‑140 (MQ=255)                                                            gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGAc                                                                         >  1:692477/1‑140 (MQ=255)                                                               cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa                                                                              >  1:1260344/1‑132 (MQ=255)                                                               cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa                                                                              <  2:1260344/132‑1 (MQ=255)                                                               cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGa                                                                          <  2:1213964/136‑1 (MQ=255)                                                               cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGa                                                                          >  1:1213964/1‑136 (MQ=255)                                                               cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGAt                                                                      >  1:342466/1‑140 (MQ=255)                                                                ggATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATc                                                                     >  2:504509/1‑140 (MQ=255)                                                                      gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGt                                                               <  1:95512/140‑1 (MQ=255)                                                                      gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGt                                                               >  2:650814/1‑140 (MQ=255)                                                                          tCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTcgcg                                                           >  2:1005470/1‑140 (MQ=255)                                                                           ccAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCgcgc                                                          >  1:1171085/1‑140 (MQ=255)                                                                               acaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCa                                                      >  2:1055431/1‑140 (MQ=255)                                                                               acaaaTCCCGCTTGGCATC‑‑TGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAAc                                                    >  1:1170170/1‑140 (MQ=255)                                                                                 aaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAAc                                                    >  2:512601/1‑140 (MQ=255)                                                                                    tCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTgg                                                 >  1:824957/1‑140 (MQ=255)                                                                                            gccATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGa                                         <  2:1001350/138‑1 (MQ=255)                                                                                             gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                        >  1:80320/1‑140 (MQ=255)                                                                                             gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                        >  1:301136/1‑140 (MQ=255)                                                                                                    tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                 <  1:1055431/140‑1 (MQ=255)                                                                                                    tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                 <  2:93332/140‑1 (MQ=255)                                                                                                    tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                 <  2:301136/140‑1 (MQ=255)                                                                                                        aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                             <  2:1171085/140‑1 (MQ=255)                                                                                                         aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                            <  1:27551/140‑1 (MQ=255)                                                                                                           gcgcTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCt                          >  2:71726/1‑140 (MQ=255)                                                                                                            cgcTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                         <  1:341690/140‑1 (MQ=255)                                                                                                              cTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                       >  1:702803/1‑140 (MQ=255)                                                                                                              cTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                       >  2:951356/1‑140 (MQ=255)                                                                                                                         ggAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttg            >  2:1278746/1‑140 (MQ=255)                                                                                                                             tGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa        >  1:1209698/1‑140 (MQ=255)                                                                                                                             tGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa        >  1:1077556/1‑140 (MQ=255)                                                                                                                             tGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa        >  2:1002939/1‑140 (MQ=255)                                                                                                                               ctggctggAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa      <  1:1005470/140‑1 (MQ=255)                                                                                                                                   ctggAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCgg  >  1:1218400/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGG  >  NC_000913/4422779‑4423048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑