Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,443	A→G	Y230C (TAC→TGC)	cysQ →	3'(2'),5'‑bisphosphate nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,443	0	A	G	100.0%	266.0 / NA	76	Y230C (TAC→TGC)	cysQ	3'(2'),5'‑bisphosphate nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/42);  total (34/42)

GTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTACACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAGAGATTG  >  NC_000913/4437313‑4437579                                                                                                                                   |                                                                                                                                         gtggCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCcgcg                                                                                                                                 <  1:70544/140‑1 (MQ=255)  tggCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGt                                                                                                                                >  1:219236/1‑140 (MQ=255)     cGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAg                                                                                                                             >  1:877568/1‑140 (MQ=255)       gAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTc                                                                                                                           >  1:1267741/1‑140 (MQ=255)        aaGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCg                                                                                                                          <  2:474681/140‑1 (MQ=255)           gACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTc                                                                                                                       >  1:463505/1‑140 (MQ=255)           gACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTc                                                                                                                       >  1:1422453/1‑140 (MQ=255)            aCAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTcc                                                                                                                      <  1:1012657/140‑1 (MQ=255)              aGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTg                                                                                                                    >  1:637085/1‑140 (MQ=255)                gcgcAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGaa                                                                                                                  >  1:1282874/1‑140 (MQ=255)                   cAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATcc                                                                                                               <  2:510845/140‑1 (MQ=255)                   cAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATcc                                                                                                               <  1:1231640/140‑1 (MQ=255)                   cAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATcc                                                                                                               >  2:372365/1‑140 (MQ=255)                    aGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCg                                                                                                              <  1:832056/140‑1 (MQ=255)                     gCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCgg                                                                                                             >  2:1383110/1‑140 (MQ=255)                         tACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGtt                                                                                                         <  2:1267741/140‑1 (MQ=255)                         tACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGtt                                                                                                         <  2:1200470/140‑1 (MQ=255)                         tACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGtt                                                                                                         <  1:845529/140‑1 (MQ=255)                         tACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGtt                                                                                                         <  1:1173528/140‑1 (MQ=255)                          aCCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTc                                                                                                        <  1:772874/140‑1 (MQ=255)                          aCCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGACAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTc                                                                                                        >  1:449641/1‑140 (MQ=255)                           cccGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAAt                                                                                                                 <  2:438201/130‑1 (MQ=255)                           cccGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAAt                                                                                                                 >  1:438201/1‑130 (MQ=255)                           cccGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCa                                                                                                       >  1:69187/1‑140 (MQ=255)                            ccGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCag                                                                                                      <  1:477735/140‑1 (MQ=255)                              gcgcTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCagag                                                                                                    <  2:382080/140‑1 (MQ=255)                              gcgcTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCagag                                                                                                    <  1:887630/140‑1 (MQ=255)                               cgcTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGACAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAgt                                                                                                   <  2:449641/140‑1 (MQ=255)                                gcTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAgtg                                                                                                  >  2:7605/1‑140 (MQ=255)                                gcTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAgtg                                                                                                  >  1:384430/1‑140 (MQ=255)                                   tCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATcc                                                                                                               <  1:591244/124‑1 (MQ=255)                                   tCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATcc                                                                                                               >  2:591244/1‑124 (MQ=255)                                   tCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCagag                                                                                                    <  1:694197/135‑1 (MQ=255)                                   tCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCagag                                                                                                    >  2:694197/1‑135 (MQ=255)                                   tCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCt                                                                                               >  1:36016/1‑140 (MQ=255)                                     ggACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTAt                                                                                             <  1:1386994/140‑1 (MQ=255)                                            cGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTaa                                                                                      <  1:372365/140‑1 (MQ=255)                                                  tttGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAg                                                                                <  2:1422453/140‑1 (MQ=255)                                                   ttGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGa                                                                               >  2:933413/1‑140 (MQ=255)                                                    tGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGAt                                                                              <  2:69187/140‑1 (MQ=255)                                                    tGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGAt                                                                              <  1:64739/140‑1 (MQ=255)                                                         cacCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAg                                                                         <  2:36016/140‑1 (MQ=255)                                                         cacCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAg                                                                         <  2:384430/140‑1 (MQ=255)                                                         cacCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGAGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAg                                                                         <  2:6827/140‑1 (MQ=255)                                                          acCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGa                                                                        >  1:391448/1‑140 (MQ=255)                                                          acCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGa                                                                        <  2:391448/140‑1 (MQ=255)                                                           ccgccgCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGaa                                                                       <  1:617438/140‑1 (MQ=255)                                                             gccgcTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAAc                                                                     <  2:637085/140‑1 (MQ=255)                                                                 cTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAgtg                                                                 >  1:813612/1‑140 (MQ=255)                                                                           tGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGa                                                       <  1:965653/140‑1 (MQ=255)                                                                            gTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGAt                                                      >  1:1126361/1‑140 (MQ=255)                                                                               gctgcagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCt                                                   >  2:1352625/1‑140 (MQ=255)                                                                                ctgcagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTg                                                  <  2:881156/140‑1 (MQ=255)                                                                                   cagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGa                                                       >  1:78629/1‑132 (MQ=255)                                                                                   cagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGa                                                       <  2:78629/132‑1 (MQ=255)                                                                                   cagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCa                                               >  2:992584/1‑140 (MQ=255)                                                                                   cagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCa                                               >  1:1074470/1‑140 (MQ=255)                                                                                   cagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCa                                               >  1:249947/1‑140 (MQ=255)                                                                                   cagctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCa                                               >  1:630585/1‑140 (MQ=255)                                                                                     gctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATc                                             >  2:327717/1‑140 (MQ=255)                                                                                     gctgcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATc                                             >  1:924520/1‑140 (MQ=255)                                                                                        gcCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTg                                          >  2:658412/1‑140 (MQ=255)                                                                                         ccGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGa                                         >  1:895425/1‑140 (MQ=255)                                                                                          cGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGAt                                        <  2:895425/140‑1 (MQ=255)                                                                                               cgcACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTtctc                                   <  2:924520/140‑1 (MQ=255)                                                                                                 cACGTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTccc                                 >  2:80230/1‑140 (MQ=255)                                                                                                    gTTCACGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAAc                              <  1:356446/140‑1 (MQ=255)                                                                                                         cGACTGGCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTaaa                         <  1:327717/140‑1 (MQ=255)                                                                                                              ggCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGtt                    >  1:303240/1‑140 (MQ=255)                                                                                                               gCAGGGTAAACCGCTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATCTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTta                   <  2:1282874/140‑1 (MQ=255)                                                                                                                            cTGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAagag      <  1:7605/140‑1 (MQ=255)                                                                                                                             tGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAgaga     <  1:992584/140‑1 (MQ=255)                                                                                                                             tGGATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAgaga     >  2:1200184/1‑140 (MQ=255)                                                                                                                              ggATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAGAGAt    <  1:501741/140‑1 (MQ=255)                                                                                                                              ggATTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAGAGAt    <  1:933413/140‑1 (MQ=255)                                                                                                                                aTTGCACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAGAGATTg  <  2:877568/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GTGGCGGAAGGACAGGCGCAGCTGTACCCGCGCTTCGGACCAACGAATATTTGGGACACCGCCGCTGGACATGCTGTAGCTGCAGCTGCCGGAGCGCACGTTCACGACTGGCAGGGTAAACCGCTGGATTACACTCCGCGTGAGTCGTTCCTGAATCCGGGGTTCAGAGTGTCTATTTACTAAATTCAGATGGCAGAAACAGTGTATTTCCTGATTCTGCCATCCTGATTTCTCCCAACCTAAAAAGTTATAAATAAAAAGAGATTG  >  NC_000913/4437313‑4437579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑