Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,452,352	G→A	G432D (GGC→GAC)	ytfR →	galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,452,352	0	G	A	100.0%	347.8 / NA	98	G432D (GGC→GAC)	ytfR	galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/51);  total (47/51)

CGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGGCATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATGCGCGAT  >  NC_000913/4452216‑4452488                                                                                                                                          |                                                                                                                                         cGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACAt                                                                                                                                       <  1:322071/140‑1 (MQ=255)   ccAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTg                                                                                                                                     >  1:123742/1‑140 (MQ=255)     aGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGAt                                                                                                                                   >  1:1164588/1‑140 (MQ=255)       cTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGa                                                                                                                                    >  2:15690/1‑137 (MQ=255)       cTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGa                                                                                                                                    <  1:15690/137‑1 (MQ=255)       cTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGt                                                                                                                                 >  1:391238/1‑140 (MQ=255)       cTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCATCGATGAGCCAACCCGCGACATTGATGt                                                                                                                                 >  1:1152859/1‑140 (MQ=255)         tGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTg                                                                                                                               >  1:40862/1‑140 (MQ=255)             aTTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGc                                                                                                                           <  1:380808/140‑1 (MQ=255)              ttCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGcc                                                                                                                          <  1:579882/140‑1 (MQ=255)              ttCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGcc                                                                                                                          <  1:1444320/140‑1 (MQ=255)              ttCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGcc                                                                                                                          <  2:391238/140‑1 (MQ=255)                 gCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCAc                                                                                                                       >  1:128833/1‑140 (MQ=255)                  cacaCCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGcc                                                                                                                          >  1:611/1‑136 (MQ=255)                  cacaCCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGcc                                                                                                                          <  2:611/136‑1 (MQ=255)                  cacaCCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACg                                                                                                                      <  1:941032/140‑1 (MQ=255)                  cacaCCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACg                                                                                                                      <  2:431975/140‑1 (MQ=255)                    cacCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGcc                                                                                                                    >  2:45726/1‑140 (MQ=255)                     acCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCg                                                                                                                   >  2:1015848/1‑140 (MQ=255)                     acCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCg                                                                                                                   <  1:1015848/140‑1 (MQ=255)                      ccTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCga                                                                                                                  <  1:644986/140‑1 (MQ=255)                         tCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTgg                                                                                                                              <  2:572951/125‑1 (MQ=255)                         tCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTgg                                                                                                                              >  1:572951/1‑125 (MQ=255)                         tCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGat                                                                                                               >  1:174107/1‑140 (MQ=255)                         tCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGat                                                                                                               >  2:237641/1‑140 (MQ=255)                         tCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGat                                                                                                               >  2:772617/1‑140 (MQ=255)                          cAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGatc                                                                                                              >  2:105937/1‑140 (MQ=255)                          cAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACC‑CAATTTCTGATCCTCGATGAGCCAACCCGCCACAATGATGTTGGGGCCCACGCCGAGatca                                                                                                             >  1:775714/1‑140 (MQ=255)                            aCTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGatcat                                                                                                            <  1:675276/140‑1 (MQ=255)                             cTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGatcatc                                                                                                           <  2:1277639/140‑1 (MQ=255)                               gAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCg                                                                                                         >  2:1402051/1‑140 (MQ=255)                               gAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCg                                                                                                         >  2:1436808/1‑140 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCga                                                                                                                  <  2:946939/129‑1 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCga                                                                                                                  >  1:946939/1‑129 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGcc                                                                                                       >  2:1165966/1‑140 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGcc                                                                                                       >  2:1445996/1‑140 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGcc                                                                                                       >  1:423538/1‑140 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGcc                                                                                                       >  1:61664/1‑140 (MQ=255)                                 acaacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGcc                                                                                                       >  2:452768/1‑140 (MQ=255)                                  caacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCt                                                                                                      <  2:1119502/140‑1 (MQ=255)                                  caacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCt                                                                                                      <  2:174107/140‑1 (MQ=255)                                  caacCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCt                                                                                                      <  2:299181/140‑1 (MQ=255)                                       gATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTg                                                                                                 >  2:497814/1‑140 (MQ=255)                                       gATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTg                                                                                                 >  2:994231/1‑140 (MQ=255)                                        aTTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGa                                                                                                >  2:423553/1‑140 (MQ=255)                                          tGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGaaa                                                                                              >  1:1268058/1‑140 (MQ=255)                                               ttCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTa                                                                                         <  1:423553/140‑1 (MQ=255)                                                 ctctcCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCATCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATg                                                                                       <  2:1152859/140‑1 (MQ=255)                                                      cggcggCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCg                                                                                  >  2:1094133/1‑140 (MQ=255)                                                       ggcggcAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGa                                                                                 <  1:237641/140‑1 (MQ=255)                                                       ggcggcAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGa                                                                                 >  2:233991/1‑140 (MQ=255)                                                         cggcAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATg                                                                               <  2:61664/140‑1 (MQ=255)                                                            cAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCTGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTc                                                                            >  2:1446569/1‑140 (MQ=255)                                                               tCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTgg                                                                         <  1:45726/140‑1 (MQ=255)                                                                 aGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGgcg                                                                       <  1:1094133/140‑1 (MQ=255)                                                                 aGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGACGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGgcg                                                                       <  1:233991/140‑1 (MQ=255)                                                                  ggaaaaaGTTGTGCCTTCCCGTTGGCCTCCTGACCCG‑CCCCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGgcgc                                                                      <  2:775714/138‑1 (MQ=255)                                                                  gCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGgcgc                                                                      <  1:611809/140‑1 (MQ=255)                                                                   cAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCgct                                                                     <  2:123742/140‑1 (MQ=255)                                                                    aaaaaGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCgctg                                                                    <  2:1164588/140‑1 (MQ=255)                                                                    aaaaaGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCgctg                                                                    <  1:49681/140‑1 (MQ=255)                                                                     aaaaGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCgctgc                                                                   <  2:1268058/140‑1 (MQ=255)                                                                          tgtTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGgcgc                                                                      <  2:841826/132‑1 (MQ=255)                                                                          tgtTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGgcgc                                                                      >  1:841826/1‑132 (MQ=255)                                                                               cTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCgctg                                                                    <  1:1116310/129‑1 (MQ=255)                                                                               cTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCgctg                                                                    >  2:1116310/1‑129 (MQ=255)                                                                                tttCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATctcc                                                        <  2:593773/140‑1 (MQ=255)                                                                                 ttCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATctcct                                                       <  1:1402051/140‑1 (MQ=255)                                                                                  tCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATctcctc                                                      >  1:413710/1‑140 (MQ=255)                                                                                                tGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCTGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAagag                                         <  1:1446569/140‑1 (MQ=255)                                                                                                   cccGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAATCGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCGCCTCCGATCTGGAAGAGCtg                                      <  2:415778/140‑1 (MQ=255)                                                                                                   cccGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtg                                      <  1:497814/140‑1 (MQ=255)                                                                                                   cccGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtg                                      <  1:1165966/140‑1 (MQ=255)                                                                                                     cGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTCGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggt                                    <  2:128833/140‑1 (MQ=255)                                                                                                      gACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtg                                   >  1:1181937/1‑140 (MQ=255)                                                                                                      gACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtg                                   >  1:54434/1‑140 (MQ=255)                                                                                                       aCCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATTCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtgg                                  >  2:1287883/1‑140 (MQ=255)                                                                                                       aCCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtgg                                  >  1:122231/1‑140 (MQ=255)                                                                                                       aCCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtgg                                  >  1:1330509/1‑140 (MQ=255)                                                                                                       aCCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtgg                                  >  2:105357/1‑140 (MQ=255)                                                                                                       aCCGCAATTTCTGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCtggtgg                                  >  2:348472/1‑140 (MQ=255)                                                                                                                  tGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGAt                       <  2:54434/140‑1 (MQ=255)                                                                                                                  tGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGAt                       <  1:452768/140‑1 (MQ=255)                                                                                                                  tGATCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGAt                       <  2:1181937/140‑1 (MQ=255)                                                                                                                    aTCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCg                     <  2:423538/140‑1 (MQ=255)                                                                                                                    aTCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCg                     <  2:122231/140‑1 (MQ=255)                                                                                                                     tCCTCGATGAGCCAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGGTATGCCGATCgg                    >  2:907634/1‑140 (MQ=255)                                                                                                                                 cAACCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATg        >  1:322012/1‑140 (MQ=255)                                                                                                                                   aCCCGCGACATTGATGTTGGTGCCCACGCCTAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATgcg      <  2:903530/140‑1 (MQ=255)                                                                                                                                   aCCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATgcg      >  1:903530/1‑140 (MQ=255)                                                                                                                                   aCCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATgcg      >  2:753554/1‑140 (MQ=255)                                                                                                                                   aCCCGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATgcg      <  2:413710/140‑1 (MQ=255)                                                                                                                                    cccGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGAt                       >  2:920473/1‑122 (MQ=255)                                                                                                                                    cccGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGAt                       <  1:920473/122‑1 (MQ=255)                                                                                                                                    cccGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATgcgc     >  2:1388395/1‑140 (MQ=255)                                                                                                                                     ccGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCg                     >  2:969715/1‑123 (MQ=255)                                                                                                                                     ccGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATGcgcg    <  1:105937/140‑1 (MQ=255)                                                                                                                                     ccGCGACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCGCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCg                     <  1:969715/123‑1 (MQ=255)                                                                                                                                      cgcgACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATGCGCGa   <  1:994231/140‑1 (MQ=255)                                                                                                                                       gcgACATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATGCGCGAt  <  2:1330509/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGATTGAATTTCTCTCCGGCGGCAATCAGCAAAAAGTGTTGCTTTCACGTTGG‑CTACTGACCCGACCGCAATTTCTGATCCTCGATGAGCCAACCCGCGGCATTGATGTTGGTGCCCACGCCGAGATCATCCGCCTGATTGAAACGCTATGCGCCGATGGTCTGGCGCTGCTGGTGATCTCCTCCGAACTGGAAGAGCTGGTGGGCTATGCCGATCGGGTGATTATCATGCGCGAT  >  NC_000913/4452216‑4452488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑