Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,518,648	T→C	L41P (CTA→CCA)	insA‑7 →	KpLE2 phage‑like element; IS1 repressor protein InsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,648	0	T	C	100.0%	300.0 / NA	92	L41P (CTA→CCA)	insA‑7	KpLE2 phage‑like element; IS1 repressor protein InsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/47);  total (45/47)

ATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAA  >  NC_000913/4518511‑4518782                                                                                                                                          |                                                                                                                                       aTTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCAc                                                                                                                                      >  1:606224/1‑140 (MQ=255)   tttAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAg                                                                                                                                    <  1:571802/140‑1 (MQ=255)   tttAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAg                                                                                                                                    <  2:273907/140‑1 (MQ=255)   tttAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAg                                                                                                                                    <  2:231429/140‑1 (MQ=255)   tttAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAg                                                                                                                                    <  2:606224/140‑1 (MQ=255)      aaGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTc                                                                                                                                 >  1:1378517/1‑140 (MQ=255)           gCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTa                                                                                                                            >  1:134815/1‑140 (MQ=255)              tGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTacac                                                                                                                         >  1:704366/1‑140 (MQ=255)               gCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACAcc                                                                                                                        >  1:1160768/1‑140 (MQ=255)                  tGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGcc                                                                                                                     >  2:1153360/1‑140 (MQ=255)                   ggCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCct                                                                                                                    >  1:1240730/1‑140 (MQ=255)                     cTTACATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCctct                                                                                                                  <  1:642911/140‑1 (MQ=255)                               catCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGc                                                                                                              >  1:1174517/1‑134 (MQ=255)                               catCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGc                                                                                                              <  2:1174517/134‑1 (MQ=255)                               catCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTa                                                                                                        >  1:1219534/1‑140 (MQ=255)                                 tCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGc                                                                                                              >  2:467568/1‑132 (MQ=255)                                 tCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGc                                                                                                              <  1:467568/132‑1 (MQ=255)                                  cAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGc                                                                                                     <  1:1153360/140‑1 (MQ=255)                                      tGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCg                                                                                                           <  1:1002224/130‑1 (MQ=255)                                      tGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCg                                                                                                           >  2:1002224/1‑130 (MQ=255)                                       gTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAg                                                                                                >  1:507429/1‑140 (MQ=255)                                        tccttcctGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGCCATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGa                                                                                               <  1:1025268/140‑1 (MQ=255)                                        tccttcctGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGa                                                                                               <  2:531342/140‑1 (MQ=255)                                        tccttcctGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGa                                                                                               <  1:70966/140‑1 (MQ=255)                                        tccttcctGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGa                                                                                               <  1:669689/140‑1 (MQ=255)                                         ccttcctGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGaa                                                                                              <  2:704366/140‑1 (MQ=255)                                             cctGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATc                                                                                          >  1:710110/1‑140 (MQ=255)                                               tGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCAt                                                                                        >  1:1475253/1‑140 (MQ=255)                                               tGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCAt                                                                                        <  2:710110/140‑1 (MQ=255)                                               tGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCAt                                                                                        >  2:1232952/1‑140 (MQ=255)                                                  tCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGa                                                                                     >  1:854333/1‑140 (MQ=255)                                                   ccGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGat                                                                                    >  1:955862/1‑140 (MQ=255)                                                   ccGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGat                                                                                    >  2:104866/1‑140 (MQ=255)                                                   ccGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGat                                                                                    <  2:1219534/140‑1 (MQ=255)                                                   ccGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGat                                                                                    <  2:1240730/140‑1 (MQ=255)                                                    cGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   <  2:1160768/140‑1 (MQ=255)                                                    cGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   >  1:1017186/1‑140 (MQ=255)                                                    cGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   <  1:1343132/140‑1 (MQ=255)                                                    cGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   >  2:265174/1‑140 (MQ=255)                                                    cGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   <  2:155366/140‑1 (MQ=255)                                                    cGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   <  1:205396/140‑1 (MQ=255)                                                     gCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGatat                                                                                  >  2:973490/1‑140 (MQ=255)                                                      cTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGa                                                                                               >  1:207886/1‑126 (MQ=255)                                                      cTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGa                                                                                               <  2:207886/126‑1 (MQ=255)                                                      cTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATg                                                                                 <  1:973490/140‑1 (MQ=255)                                                      cTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATg                                                                                 >  1:993839/1‑140 (MQ=255)                                                        ccTGAAGGCGTGGTGCGTAACGGAAAAAGCACTGCCGGACACCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGc                                                                               <  2:1475253/139‑1 (MQ=255)                                                                 gtggtgCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATgg                                                                      >  1:336456/1‑140 (MQ=255)                                                                   ggtgCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   >  1:1216803/1‑125 (MQ=255)                                                                   ggtgCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGata                                                                                   <  2:1216803/125‑1 (MQ=255)                                                                         tAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGAt                                                              >  1:49727/1‑140 (MQ=255)                                                                               cAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGaa                                                                         >  2:998247/1‑123 (MQ=255)                                                                               cAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGaa                                                                         <  1:998247/123‑1 (MQ=255)                                                                               cAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTcgcg                                                        >  1:1008661/1‑140 (MQ=255)                                                                               cAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTcgcg                                                        >  2:1379464/1‑140 (MQ=255)                                                                                aaaaGCCCGGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCGAAAAAACATGGCAACCACATTTCACTTCCACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCgcgc                                                       <  2:955862/140‑1 (MQ=17)                                                                                   aGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAg                                                    <  2:1301100/140‑1 (MQ=255)                                                                                     cACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTg                                                  <  1:1131232/140‑1 (MQ=255)                                                                                      aCTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGc                                                 <  2:1008661/140‑1 (MQ=255)                                                                                      aCTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGc                                                 <  1:104866/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  2:43620/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  1:1346323/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  1:592316/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  2:49727/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  1:1232952/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  2:573833/140‑1 (MQ=255)                                                                                       cTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAAAATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCa                                                <  2:1378517/140‑1 (MQ=255)                                                                                         gCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACg                                              <  2:993839/140‑1 (MQ=255)                                                                                          ccGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGc                                             >  2:1406476/1‑140 (MQ=255)                                                                                               cATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACCTGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTAt                                        <  2:1017186/140‑1 (MQ=255)                                                                                                    gcgcTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCg                                   <  1:268608/140‑1 (MQ=255)                                                                                                      gcTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGtt                                 <  2:750882/140‑1 (MQ=255)                                                                                                       cTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTg                                >  1:425094/1‑140 (MQ=255)                                                                                                          tctcTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCAc                                               >  2:676590/1‑122 (MQ=255)                                                                                                          tctcTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCAc                                               <  1:676590/122‑1 (MQ=255)                                                                                                          tctcTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGt                                  >  1:1221906/1‑135 (MQ=255)                                                                                                          tctcTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGt                                  <  2:1221906/135‑1 (MQ=255)                                                                                                          tctcTGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGcc                             >  2:1148213/1‑140 (MQ=255)                                                                                                           ctctGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCt                            <  2:421904/140‑1 (MQ=255)                                                                                                              tGCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCaa                         <  2:134815/140‑1 (MQ=255)                                                                                                               gCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAac                        >  2:1075706/1‑140 (MQ=255)                                                                                                               gCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAac                        <  1:1075706/140‑1 (MQ=255)                                                                                                               gCTCTCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAac                        >  1:709202/1‑140 (MQ=255)                                                                                                                ctctCCTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAaca                       <  2:420848/140‑1 (MQ=255)                                                                                                                    ccTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAaca                       <  1:142648/136‑1 (MQ=255)                                                                                                                    ccTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAaca                       >  2:142648/1‑136 (MQ=255)                                                                                                                    ccTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGt                   >  2:1465086/1‑140 (MQ=255)                                                                                                                    ccTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGt                   >  1:1457971/1‑140 (MQ=255)                                                                                                                    ccTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGt                   >  1:1415133/1‑140 (MQ=255)                                                                                                                     cTTGCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGtt                  >  1:1286476/1‑140 (MQ=255)                                                                                                                        gCCGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTa               >  2:564249/1‑140 (MQ=255)                                                                                                                          cGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCt                            <  2:423574/125‑1 (MQ=255)                                                                                                                          cGTAAAACATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCt                            >  1:423574/1‑125 (MQ=255)                                                                                                                               aaCATGGCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACt        >  1:1253149/1‑140 (MQ=255)                                                                                                                                     gCAACCACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTaaaaa  >  1:491320/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCCTTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTAAGCACCAGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAA  >  NC_000913/4518511‑4518782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑