Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,592,474	G→A	G273E (GGG→GAG)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,592,474	0	G	A	100.0%	348.4 / NA	98	G273E (GGG→GAG)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (54/44);  total (54/44)

GCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGAAGCAG  >  NC_000913/4592339‑4592610                                                                                                                                        |                                                                                                                                         gCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGcc                                                                                                                                      >  1:1318706/1‑140 (MQ=255)  cATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCa                                                                                                                                     <  1:991178/140‑1 (MQ=255)  cATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCa                                                                                                                                     <  2:515616/140‑1 (MQ=255)    ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGGGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAAt                                                                                                                                   <  1:489889/140‑1 (MQ=255)    ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAAt                                                                                                                                   >  1:506787/1‑140 (MQ=255)    ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAAt                                                                                                                                   >  2:314416/1‑140 (MQ=255)    ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAAt                                                                                                                                   >  2:1009050/1‑140 (MQ=255)     tCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATg                                                                                                                                  <  2:1401410/140‑1 (MQ=255)       gTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGcc                                                                                                                                <  1:1009050/140‑1 (MQ=255)       gTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGcc                                                                                                                                <  2:313004/140‑1 (MQ=255)       gTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGcc                                                                                                                                <  2:240034/140‑1 (MQ=255)       gTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGcc                                                                                                                                <  1:314416/140‑1 (MQ=255)       gTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGcc                                                                                                                                <  1:256165/140‑1 (MQ=255)            atTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCta                                                                                                                           >  1:48618/1‑140 (MQ=255)            atTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCta                                                                                                                           >  1:1145648/1‑140 (MQ=255)             ttGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCtat                                                                                                                          >  2:1209763/1‑140 (MQ=255)               gCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGGCATCTATAg                                                                                                                        <  1:448189/140‑1 (MQ=255)                 aGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCg                                                                                                                      >  2:389816/1‑140 (MQ=255)                   gcggcgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGt                                                                                                                    >  2:1166062/1‑140 (MQ=255)                   gcggcgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGt                                                                                                                    >  2:972942/1‑140 (MQ=255)                        gATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGt                                                                                                                    <  2:1472376/135‑1 (MQ=255)                        gATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGt                                                                                                                    >  1:1472376/1‑135 (MQ=255)                        gATCTGGTGAAACCGATTGAGGTGGATGGCTATAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCCGGGAGAGCTGATGCGTACCGTCGGTGAAGTGCGCAACGAGGCCAATGCCATCTATAGCGGCGCCac                                                                                                               >  2:190832/1‑139 (MQ=255)                                  aaCCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGcc                                                                                                     <  1:1209763/140‑1 (MQ=255)                                    ccGATTGAGGTGGATGGCTATAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAc                                                                                                   <  1:190832/140‑1 (MQ=255)                                     cGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAcc                                                                                                  <  2:75191/140‑1 (MQ=255)                                        ttGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGc                                                                                               >  2:1424769/1‑140 (MQ=255)                                           aGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAAt                                                                                            <  1:1338590/140‑1 (MQ=255)                                             gTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                                                                                          >  1:901280/1‑140 (MQ=255)                                             gTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                                                                                          <  1:312484/140‑1 (MQ=255)                                             gTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                                                                                          >  2:703751/1‑140 (MQ=255)                                             gTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                                                                                          <  1:1424769/140‑1 (MQ=255)                                                gATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGa                                                                                       >  2:1461434/1‑140 (MQ=255)                                                gATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGa                                                                                       <  2:424656/140‑1 (MQ=255)                                                 aTGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAt                                                                                      <  1:779442/140‑1 (MQ=255)                                                     ctctAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAtctct                                                                                  >  2:1212595/1‑140 (MQ=255)                                                     ctctAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAtctct                                                                                  >  1:913287/1‑140 (MQ=255)                                                        tAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGc                                                                                               >  1:998648/1‑124 (MQ=255)                                                        tAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGc                                                                                               <  2:998648/124‑1 (MQ=255)                                                          aTGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                                                                                          >  2:42133/1‑127 (MQ=255)                                                          aTGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATaa                                                                                          <  1:42133/127‑1 (MQ=255)                                                           tGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgc                                                                            >  2:1105814/1‑140 (MQ=255)                                                            gagaTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGTCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgcg                                                                           <  2:1318706/140‑1 (MQ=255)                                                            gagaTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgcg                                                                           <  2:678451/140‑1 (MQ=255)                                                            gagaTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgcg                                                                           <  1:1166062/140‑1 (MQ=255)                                                              gaTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCa                                                                         <  1:1461434/140‑1 (MQ=255)                                                               aTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCAc                                                                        >  2:1204198/1‑140 (MQ=255)                                                               aTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCAc                                                                        >  1:194641/1‑140 (MQ=255)                                                               aTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCAc                                                                        >  2:361921/1‑140 (MQ=255)                                                               aTGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCAc                                                                        >  1:1149504/1‑140 (MQ=255)                                                                   ggCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAg                                                                    <  2:48618/140‑1 (MQ=255)                                                                      aaCTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCaa                                                                 >  1:1443614/1‑140 (MQ=255)                                                                            cAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGcc                                                           >  1:605923/1‑140 (MQ=255)                                                                             agagagTTTGCGCCATATGCAGGGAGAGCTGATGTGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCg                                                          >  2:99501/1‑140 (MQ=255)                                                                             agagagTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCg                                                          <  2:846652/140‑1 (MQ=255)                                                                             agagagTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCg                                                          >  1:846652/1‑140 (MQ=255)                                                                               agagTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCt                                                        <  1:972942/140‑1 (MQ=255)                                                                                  gTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCg                                                     >  1:602011/1‑140 (MQ=255)                                                                                     tGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTg                                                  >  2:1088624/1‑140 (MQ=255)                                                                                     tGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTg                                                  >  2:1422266/1‑140 (MQ=255)                                                                                            tatGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAgaga                                           >  1:577879/1‑140 (MQ=255)                                                                                             atGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGAc                                          <  1:1204198/140‑1 (MQ=255)                                                                                              tGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACg                                         <  2:1145648/140‑1 (MQ=255)                                                                                              tGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACg                                         <  2:913287/140‑1 (MQ=255)                                                                                               gCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACgg                                        <  1:1088624/140‑1 (MQ=255)                                                                                                cAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGc                                       <  2:577879/140‑1 (MQ=255)                                                                                                    gagaGCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagcc                                   >  2:434056/1‑140 (MQ=255)                                                                                                    gagaGCTGATGCGTACCGTCGGTGATGAGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagcc                                   >  2:109633/1‑140 (MQ=255)                                                                                                      gagCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagccag                                 <  2:194641/140‑1 (MQ=255)                                                                                                      gagCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagccag                                 <  2:605923/140‑1 (MQ=255)                                                                                                       agCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagccagc                                >  2:747117/1‑140 (MQ=255)                                                                                                       agCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagccagc                                >  2:687415/1‑140 (MQ=255)                                                                                                       agCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagccagc                                >  2:363735/1‑140 (MQ=255)                                                                                                       agCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAAGAACGATCTCGCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagccagc                                >  2:1321836/1‑140 (MQ=255)                                                                                                        gCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTTTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCa                               >  2:1355468/1‑140 (MQ=255)                                                                                                        gCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACg                                         >  1:280566/1‑130 (MQ=255)                                                                                                        gCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACg                                         <  2:280566/130‑1 (MQ=255)                                                                                                        gCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCa                               <  2:1149504/140‑1 (MQ=255)                                                                                                        gCTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCa                               >  1:216127/1‑140 (MQ=255)                                                                                                         cTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCAt                              >  1:1435926/1‑140 (MQ=255)                                                                                                         cTGATGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCAt                              >  1:1463180/1‑140 (MQ=255)                                                                                                             tGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagc                                    <  1:635456/130‑1 (MQ=255)                                                                                                             tGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcagc                                    >  2:635456/1‑130 (MQ=255)                                                                                                             tGCGTACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAg                          <  2:1443614/140‑1 (MQ=255)                                                                                                                 tACCGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACTGCAGCCAGCATGGAGCAAc                      >  1:307198/1‑140 (MQ=255)                                                                                                                    cGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcag                                     >  1:994021/1‑122 (MQ=255)                                                                                                                    cGTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGcag                                     <  2:994021/122‑1 (MQ=255)                                                                                                                     gTCGGTGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGAc                  <  2:1435926/140‑1 (MQ=255)                                                                                                                          tGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCaa             <  1:363735/140‑1 (MQ=255)                                                                                                                          tGATGTGCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCaa             <  2:1463180/140‑1 (MQ=255)                                                                                                                              gtgCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGTAGCAACTGACCGCAACGGt         >  1:340897/1‑140 (MQ=255)                                                                                                                               tgCGCAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTg        >  2:938186/1‑140 (MQ=255)                                                                                                                                 cgcAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGaa      >  2:162962/1‑140 (MQ=255)                                                                                                                                 cgcAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGaa      >  1:766634/1‑140 (MQ=255)                                                                                                                                  gcAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGAAg     >  1:1251629/1‑140 (MQ=255)                                                                                                                                  gcAACGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGAAg     >  1:1344614/1‑140 (MQ=255)                                                                                                                                    aaCGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGAAGCa   >  2:954615/1‑140 (MQ=255)                                                                                                                                     aCGAGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGAAGCAg  <  1:938186/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGCGCACCGAGCAACAGGCCGCTTCGCTGGAAGAGACGGCAGCCAGCATGGAGCAACTGACCGCAACGGTGAAGCAG  >  NC_000913/4592339‑4592610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑