Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,649	T→C	E47E (GAA→GAG)	ytjB ←	protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,649	0	T	C	100.0%	292.2 / NA	84	E47E (GAA→GAG)	ytjB	protein Smp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/36);  total (48/36)

ACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCG  >  NC_000913/4624515‑4624783                                                                                                                                       |                                                                                                                                       acacCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGctcttc                                                                                                                                   <  2:21021/140‑1 (MQ=255)  cacCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTcc                                                                                                                                  <  2:823418/140‑1 (MQ=255)    cccGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAg                                                                                                                                <  2:860398/140‑1 (MQ=255)      cgcgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCt                                                                                                                              >  1:1018661/1‑140 (MQ=255)      cgcgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCt                                                                                                                              >  2:1154992/1‑140 (MQ=255)        cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCtgt                                                                                                                            >  2:98973/1‑140 (MQ=255)        cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCtgt                                                                                                                            >  2:1355716/1‑140 (MQ=255)        cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCt                                                                                                                              <  1:902166/138‑1 (MQ=255)        cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCt                                                                                                                              >  2:902166/1‑138 (MQ=255)        cgTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCG‑TTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCtgtg                                                                                                                           >  2:279429/1‑140 (MQ=255)          tCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTgg                                                                                                                          >  1:1110902/1‑140 (MQ=255)                         tCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCtg                                                                                                           <  1:1306489/140‑1 (MQ=255)                              cGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTTATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTggtggt                                                                                                      <  2:1018661/140‑1 (MQ=255)                              cGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTggtggt                                                                                                      <  1:594391/140‑1 (MQ=255)                               gTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGtt                                                                                                     <  2:608097/140‑1 (MQ=255)                                ttAACTGATCGAGGATCGCCTGAATGCGTTTTTCGTCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGttt                                                                                                    >  2:1309879/1‑140 (MQ=255)                                ttAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGttt                                                                                                    >  1:758794/1‑140 (MQ=255)                                ttAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGttt                                                                                                    >  2:1443237/1‑140 (MQ=255)                                   aCTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGa                                                                                                 <  2:560608/140‑1 (MQ=255)                                       aTCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaa                                                                                             >  2:1370279/1‑140 (MQ=255)                                       aTCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaa                                                                                             >  1:862896/1‑140 (MQ=255)                                       aTCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaa                                                                                             >  1:341060/1‑140 (MQ=255)                                       aTCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaa                                                                                             >  2:137181/1‑140 (MQ=255)                                       aTCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaa                                                                                             >  2:583578/1‑140 (MQ=255)                                       aTCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaa                                                                                             >  1:1400506/1‑140 (MQ=255)                                        tCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTaaa                                                                                            >  1:815024/1‑140 (MQ=255)                                          gAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAAcc                                                                                          <  1:583578/140‑1 (MQ=255)                                             gATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATg                                                                                       >  2:1003928/1‑140 (MQ=255)                                             gATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATg                                                                                       <  2:1110902/140‑1 (MQ=255)                                              aTCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTGAACCATGa                                                                                      <  1:913260/140‑1 (MQ=255)                                               tCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGAc                                                                                     >  2:360130/1‑140 (MQ=255)                                                cGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                    <  1:1370279/140‑1 (MQ=255)                                                cGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                    <  2:862896/140‑1 (MQ=255)                                                 gCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGc                                                                                   >  2:20250/1‑140 (MQ=255)                                                  ccTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGa                                                                                                 <  1:582472/125‑1 (MQ=255)                                                  ccTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGa                                                                                                 >  2:582472/1‑125 (MQ=255)                                                  ccTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCt                                                                                  >  2:806368/1‑140 (MQ=255)                                                    tGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                    <  1:826425/136‑1 (MQ=255)                                                    tGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                    >  2:826425/1‑136 (MQ=255)                                                    tGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTcc                                                                                <  1:1443237/140‑1 (MQ=255)                                                      aaTGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                    <  2:320033/134‑1 (MQ=255)                                                      aaTGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                    >  1:320033/1‑134 (MQ=255)                                                      aaTGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCt                                                                              >  1:1069481/1‑140 (MQ=255)                                                            tttttCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCa                                                                        <  1:1355716/140‑1 (MQ=255)                                                            tttttCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCa                                                                        <  2:1400506/140‑1 (MQ=255)                                                             ttttCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAg                                                                       >  1:322180/1‑140 (MQ=255)                                                                tCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAgcgc                                                                    >  1:1325063/1‑140 (MQ=255)                                                                tCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAgcgc                                                                    >  2:413132/1‑140 (MQ=255)                                                                    ccGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCa                                                                           <  2:210040/129‑1 (MQ=255)                                                                    ccGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCa                                                                           >  1:210040/1‑129 (MQ=255)                                                                    ccGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACt                                                                <  2:815024/140‑1 (MQ=255)                                                                           gTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAgg                                                         <  1:413132/140‑1 (MQ=255)                                                                           gTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAgg                                                         <  1:360130/140‑1 (MQ=255)                                                                                tACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGa                                                    <  1:1234480/140‑1 (MQ=255)                                                                                  cGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACa                                                  >  2:547406/1‑140 (MQ=255)                                                                                    cATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGa                                                >  2:319168/1‑140 (MQ=255)                                                                                    cATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGa                                                <  1:547406/140‑1 (MQ=255)                                                                                    cATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGa                                                >  2:757891/1‑140 (MQ=255)                                                                                      tCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAAc                                              <  1:1003928/140‑1 (MQ=255)                                                                                      tCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAAc                                              >  1:430859/1‑140 (MQ=255)                                                                                          cGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGa                                          >  1:237420/1‑140 (MQ=255)                                                                                          cGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGa                                          <  2:341060/140‑1 (MQ=255)                                                                                          cGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGa                                          <  2:1325063/140‑1 (MQ=255)                                                                                             tGCAACGTTCAGCGTCACCTGACGCGCCGGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACaa                                       >  2:493051/1‑140 (MQ=255)                                                                                             tGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACaa                                       >  1:246557/1‑140 (MQ=255)                                                                                              gCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGaa                                               <  1:485391/131‑1 (MQ=255)                                                                                              gCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGaa                                               >  2:485391/1‑131 (MQ=255)                                                                                              gCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAAt                                      <  1:98973/140‑1 (MQ=255)                                                                                               cAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATc                                     >  2:1286923/1‑140 (MQ=255)                                                                                                aaCGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGa                                                    <  1:878739/124‑1 (MQ=255)                                                                                                aaCGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGa                                                    >  2:878739/1‑124 (MQ=255)                                                                                                    ttCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGc                                >  2:1324608/1‑140 (MQ=255)                                                                                                       aGCGTCACCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCg                             >  1:263984/1‑140 (MQ=255)                                                                                                             aCCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAg                       >  2:481112/1‑140 (MQ=255)                                                                                                             aCCTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAg                       >  2:481111/1‑140 (MQ=255)                                                                                                              ccTGACGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGc                      >  2:1287341/1‑140 (MQ=255)                                                                                                                  aCGCGCCAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGa                  >  1:707225/1‑140 (MQ=255)                                                                                                                       ccAGGGTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTc             >  2:332275/1‑140 (MQ=255)                                                                                                                           ggTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGtt         <  1:757891/140‑1 (MQ=255)                                                                                                                           ggTGCGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGtt         <  1:319168/140‑1 (MQ=255)                                                                                                                               cGGGCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgt     <  2:237420/140‑1 (MQ=255)                                                                                                                                gggCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgtg    >  2:297026/1‑140 (MQ=255)                                                                                                                                gggCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCG‑CACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGc   >  2:681687/1‑140 (MQ=255)                                                                                                                                 ggCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGc   >  2:812350/1‑140 (MQ=255)                                                                                                                                 ggCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGc   <  1:806368/140‑1 (MQ=255)                                                                                                                                  gCCAGCTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCg  <  2:1069481/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCG  >  NC_000913/4624515‑4624783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑