BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,471	C→T	R53C (CGT→TGT)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,471	0	C	T	100.0%	421.5 / NA	117	R53C (CGT→TGT)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (57/60); total (57/60)

Only 100 of 117 total aligned reads displayed.

GCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTAT  >  NC_000913/4627342‑4627607

gCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGtttttt                                                                                                                                   <  2:589946/137‑1 (MQ=255)

gCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGtttttt                                                                                                                                   >  1:589946/1‑137 (MQ=255)

 ccATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTgggg                                                                                                                               >  1:152285/1‑140 (MQ=255)

  cATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGc                                                                                                                              <  2:114516/140‑1 (MQ=255)

    tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTggggtt                                                                                                                             <  1:501715/139‑3 (MQ=255)

    tCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTggggtt                                                                                                                             >  2:501715/1‑137 (MQ=255)

              gggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTcccc                                                                                                                  >  1:781844/1‑140 (MQ=255)

               ggCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCg                                                                                                                 >  1:1305198/1‑140 (MQ=255)

                gCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCgg                                                                                                                >  1:739390/1‑140 (MQ=255)

                gCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCgg                                                                                                                <  2:812320/140‑1 (MQ=255)

                 cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGc                                                                                                               >  1:1115538/1‑140 (MQ=255)

                 cTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGc                                                                                                               <  1:896727/140‑1 (MQ=255)

                  tGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCa                                                                                                              >  1:1022187/1‑140 (MQ=255)

                         tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGaaa                                                                                                       >  2:1150600/1‑140 (MQ=255)

                         tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGaaa                                                                                                       >  2:48168/1‑140 (MQ=255)

                         tACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGaaa                                                                                                       >  1:127485/1‑140 (MQ=255)

                           cagcagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAAc                                                                                                     >  1:193059/1‑140 (MQ=255)

                              cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGAt                                                                                                  <  1:833164/140‑1 (MQ=255)

                              cagGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTCTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGAt                                                                                                  <  2:1305198/140‑1 (MQ=255)

                                 gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCgg                                                                                               >  2:923941/1‑140 (MQ=255)

                                 gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCgg                                                                                               >  2:1468076/1‑140 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGAt                                                                                                  <  1:628556/135‑1 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGAt                                                                                                  >  2:628556/1‑135 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGgtg                                                                                             >  2:1025134/1‑140 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGgtg                                                                                             >  2:715988/1‑140 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGgtg                                                                                             <  1:265869/140‑1 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGgtg                                                                                             >  2:73866/1‑140 (MQ=255)

                                   aGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGgtg                                                                                             >  1:1141565/1‑140 (MQ=255)

                                     cTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTc                                                                                           >  1:916852/1‑140 (MQ=255)

                                      tGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCg                                                                                          >  1:779839/1‑140 (MQ=255)

                                            cAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCg                                                                                    >  1:1009131/1‑140 (MQ=255)

                                              gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                  <  1:1025134/140‑1 (MQ=255)

                                              gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                  <  1:180464/140‑1 (MQ=255)

                                              gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                  <  2:1424717/140‑1 (MQ=255)

                                              gCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGt                                                                                  <  1:923941/140‑1 (MQ=255)

                                                  tATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGt                                                                              >  1:886235/1‑140 (MQ=255)

                                                  tATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTT‑CCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGtt                                                                             <  2:897743/140‑1 (MQ=255)

                                                   aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGtt                                                                             <  2:766928/140‑1 (MQ=255)

                                                     gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCt                                                                           <  2:886235/140‑1 (MQ=255)

                                                     gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCt                                                                           <  1:1327639/140‑1 (MQ=255)

                                                      aCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTa                                                                          >  1:609764/1‑140 (MQ=255)

                                                       ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAc                                                                         <  1:1150600/140‑1 (MQ=255)

                                                       ccAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAc                                                                         <  2:193059/140‑1 (MQ=255)

                                                         aaaGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAccc                                                                       <  1:73866/140‑1 (MQ=255)

                                                          aaGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCa                                                                      >  2:334629/1‑140 (MQ=255)

                                                          aaGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCa                                                                      >  2:1089590/1‑140 (MQ=255)

                                                          aaGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCa                                                                      <  2:609764/140‑1 (MQ=255)

                                                               tATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTg                                                                   >  1:147379/1‑138 (MQ=255)

                                                               tATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTg                                                                   <  2:147379/138‑1 (MQ=255)

                                                                 tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAccc                                                                       >  2:1425189/1‑132 (MQ=255)

                                                                 tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAccc                                                                       <  1:1425189/132‑1 (MQ=255)

                                                                 tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATa                                                               <  1:718630/140‑1 (MQ=255)

                                                                 tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATa                                                               >  2:257945/1‑140 (MQ=255)

                                                                 tttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCACAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATa                                                               >  2:1020346/1‑140 (MQ=255)

                                                                  ttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATAc                                                              >  1:1181053/1‑140 (MQ=255)

                                                                   tAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATAcc                                                             <  2:781844/140‑1 (MQ=255)

                                                                    aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCg                                                            <  2:739390/140‑1 (MQ=255)

                                                                    aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCg                                                            <  2:1181053/140‑1 (MQ=255)

                                                                    aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCg                                                            <  1:715988/140‑1 (MQ=255)

                                                                     aGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCgg                                                           <  1:535383/140‑1 (MQ=255)

                                                                     aGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCgg                                                           <  2:1112696/140‑1 (MQ=255)

                                                                      gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACGGGa                                                          <  2:430813/140‑1 (MQ=255)

                                                                      gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                          <  2:1141565/140‑1 (MQ=255)

                                                                      gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                          <  2:127485/140‑1 (MQ=255)

                                                                      gCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTACCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                          >  1:1459536/1‑140 (MQ=255)

                                                                       ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAcc                                                                        <  2:661282/125‑1 (MQ=255)

                                                                       ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTAcc                                                                        >  1:661282/1‑125 (MQ=255)

                                                                       ccAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGAc                                                         >  2:743176/1‑140 (MQ=255)

                                                                         aGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGACCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACat                                                       >  2:761134/1‑140 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCgg                                                           <  2:447292/134‑1 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCgg                                                           >  1:447292/1‑134 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                          <  1:1027191/135‑1 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGa                                                          >  2:1027191/1‑135 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                     >  2:57633/1‑140 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                     <  2:1009131/140‑1 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                     <  1:1269961/140‑1 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                     >  2:1267693/1‑140 (MQ=255)

                                                                           ttACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                     <  1:57633/140‑1 (MQ=255)

                                                                                 aaTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCt                                               >  1:382935/1‑140 (MQ=255)

                                                                                     ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACatat                                                     >  1:441358/1‑130 (MQ=255)

                                                                                          aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcg                                      <  2:531560/140‑1 (MQ=255)

                                                                                          aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcg                                      <  1:334629/140‑1 (MQ=255)

                                                                                          aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcg                                      <  1:743176/140‑1 (MQ=255)

                                                                                           tCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcgc                                     >  1:1242316/1‑140 (MQ=255)

                                                                                           tCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAgcgc                                     >  2:730097/1‑140 (MQ=255)

                                                                                               aaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCt                                 >  1:1142362/1‑140 (MQ=255)

                                                                                                     cAGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGcc                           >  2:449355/1‑140 (MQ=255)

                                                                                                      aGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCa                          >  1:846515/1‑140 (MQ=255)

                                                                                                      aGCGCGCAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCa                          >  2:513903/1‑140 (MQ=255)

                                                                                                        cgcgcAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAgg                        <  1:257945/140‑1 (MQ=255)

                                                                                                           gcAAAAGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTg                     <  1:404451/140‑1 (MQ=255)

                                                                                                             aaaaGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGAc                   <  2:309947/140‑1 (MQ=255)

                                                                                                              aaaGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACg                  <  2:1115538/140‑1 (MQ=255)

                                                                                                              aaaGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTACCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACg                  <  2:1459536/140‑1 (MQ=255)

                                                                                                                aGCTGGAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                <  2:1454151/140‑1 (MQ=255)

                                                                                                                    ggAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                >  2:1021943/1‑136 (MQ=255)

                                                                                                                    ggAGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAg                <  1:1021943/136‑1 (MQ=255)

                                                                                                                      aGGCGTTGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCat          >  1:362670/1‑140 (MQ=255)

                                                                                                                            tGCACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCAtt    >  1:1039634/1‑140 (MQ=255)

                                                                                                                              cACTGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTAt  <  2:362670/140‑1 (MQ=255)

GCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTAT  >  NC_000913/4627342‑4627607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`