Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,156	T→C	N384D (AAC→GAC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,156	0	T	C	100.0%	273.6 / NA	79	N384D (AAC→GAC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/42);  total (37/42)

CGGTCAGACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATT  >  NC_000913/365021‑365288                                                                                                                                        |                                                                                                                                     cGGTCAGACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGTGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTc                                                                                                                                  >  1:96353/1‑140 (MQ=255)      aGACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCtt                                                                                                                             <  1:123074/140‑1 (MQ=255)       gACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTtc                                                                                                                            <  2:216073/140‑1 (MQ=255)       gACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCCTTAAAGTCGTTCTGCTtc                                                                                                                            <  2:1464629/140‑1 (MQ=255)        aCGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTtca                                                                                                                           >  1:49975/1‑140 (MQ=255)        aCGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTtca                                                                                                                           >  2:1154825/1‑140 (MQ=255)        aCGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGAAGCGGTCGCACAGCGTGTACCACAGCGTATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTtca                                                                                                                           >  2:1320553/1‑140 (MQ=255)         cGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTtcat                                                                                                                          <  1:1145658/140‑1 (MQ=255)            ttCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATcag                                                                                                                       >  1:583/1‑140 (MQ=255)            ttCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATcag                                                                                                                       >  1:1200634/1‑140 (MQ=255)            ttCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATcag                                                                                                                       <  2:69289/140‑1 (MQ=255)                ttGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAgg                                                                                                                   <  2:593522/140‑1 (MQ=255)                 tGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGa                                                                                                                  <  1:1154825/140‑1 (MQ=255)                      ccATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATcagca                                                                                                                     >  1:1367631/1‑132 (MQ=255)                      ccATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATcagca                                                                                                                     <  2:1367631/132‑1 (MQ=255)                         tGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGc                                                                                                          >  2:366150/1‑140 (MQ=255)                         tGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGc                                                                                                          >  2:311797/1‑140 (MQ=255)                          gCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACGGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCCTCATCAGCAGGATATCCTGCa                                                                                                         >  2:1343519/1‑140 (MQ=255)                            cGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCAcc                                                                                                       <  2:620042/140‑1 (MQ=255)                                 gTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGt                                                                                                  >  1:55153/1‑140 (MQ=255)                                   ttCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCt                                                                                                >  2:88980/1‑140 (MQ=255)                                      aacatTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCt                                                                                             <  2:438144/137‑1 (MQ=255)                                      aaTATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCt                                                                                             <  2:774605/140‑1 (MQ=255)                                      aaTATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCt                                                                                             <  1:1206749/140‑1 (MQ=255)                                      aaTATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGCATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCt                                                                                             <  2:1026688/140‑1 (MQ=255)                                      aaTATTGGCTTAATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCt                                                                                             <  2:1343400/140‑1 (MQ=255)                                          ttGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATc                                                                                         <  1:1339033/140‑1 (MQ=255)                                            ggCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCa                                                                                       <  1:1343519/140‑1 (MQ=255)                                            ggCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCa                                                                                       >  2:645595/1‑140 (MQ=255)                                            ggCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCa                                                                                       <  2:1200634/140‑1 (MQ=255)                                            ggCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCa                                                                                       <  2:583/140‑1 (MQ=255)                                               ttCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtga                                                                                    <  1:143722/140‑1 (MQ=255)                                                tCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgac                                                                                   >  1:1068218/1‑140 (MQ=255)                                                tCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgac                                                                                   >  2:810950/1‑140 (MQ=255)                                                 cATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgacc                                                                                  <  1:645595/140‑1 (MQ=255)                                                 cATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATACCCTGCACCATCGTCTGCTCATCCAtgacc                                                                                  <  2:671169/140‑1 (MQ=255)                                                  aTCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTc                                                                                                 <  1:1169193/124‑1 (MQ=255)                                                  aTCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTc                                                                                                 >  2:1169193/1‑124 (MQ=255)                                                  aTCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTc                                                                                                 <  1:362751/124‑1 (MQ=255)                                                  aTCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTc                                                                                                 >  2:362751/1‑124 (MQ=255)                                                  aTCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgacct                                                                                 <  2:1122508/140‑1 (MQ=255)                                                    ccaccaCATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgacctga                                                                               <  2:1406080/140‑1 (MQ=255)                                                           aTACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCa                                                                        <  1:1320553/140‑1 (MQ=255)                                                             aCAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCaga                                                                      >  1:855661/1‑140 (MQ=255)                                                             aCAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCaga                                                                      >  1:493434/1‑140 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTTACCATGCAGAGgat                                                                  >  1:120560/1‑140 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGc                                                                         <  2:1050387/133‑1 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGc                                                                         >  1:1050387/1‑133 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgat                                                                  >  2:78724/1‑140 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgat                                                                  >  1:455256/1‑140 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgat                                                                  >  2:221658/1‑140 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgat                                                                  >  1:703401/1‑140 (MQ=255)                                                                 gCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgat                                                                  >  1:849722/1‑140 (MQ=255)                                                                  ccGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgacc                                                                                  >  2:324886/1‑123 (MQ=255)                                                                  ccGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCAtgacc                                                                                  <  1:324886/123‑1 (MQ=255)                                                                  ccGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATg                                                                          <  2:562009/131‑1 (MQ=255)                                                                  ccGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATg                                                                          >  1:562009/1‑131 (MQ=255)                                                                  ccGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgatg                                                                 >  1:144892/1‑140 (MQ=255)                                                                    gTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGgatgat                                                               <  1:221658/140‑1 (MQ=255)                                                                     tAGCGGTCGCGCAGCGTACACCACACCGGATGATTCAGCTACTCCGAACAGCGCACGCCGTTAAAGTTGTTCTGCTTCGTCACCAGGATATCCTGCGCCATCGTCTGCTCATCCACGACCCGACCATGCCGAGGatgatg                                                              >  2:1187288/1‑140 (MQ=255)                                                                     tAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGatgatg                                                              >  1:1386153/1‑140 (MQ=255)                                                                     tAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGatgatg                                                              >  1:1355165/1‑140 (MQ=255)                                                                      aGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGc                                                             <  1:566412/140‑1 (MQ=255)                                                                          gTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGt                                                         <  1:78724/140‑1 (MQ=255)                                                                           tCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTg                                                        <  2:849722/140‑1 (MQ=255)                                                                            cGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGa                                                       <  2:96353/140‑1 (MQ=255)                                                                            cGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGa                                                       <  2:1078016/140‑1 (MQ=255)                                                                                 aGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGtt                                                  <  2:703401/140‑1 (MQ=255)                                                                                      gtACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGc                                             <  2:1355165/140‑1 (MQ=255)                                                                                        aCCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCt                                           <  2:1386153/140‑1 (MQ=255)                                                                                             aGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAAt                                      >  1:756034/1‑140 (MQ=255)                                                                                                ggATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAg                                   <  2:55153/140‑1 (MQ=255)                                                                                                 gATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGc                                  >  2:1232043/1‑140 (MQ=255)                                                                                                  aTGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCa                                 <  2:855661/140‑1 (MQ=255)                                                                                                       tCGGATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGc                            >  2:1431153/1‑140 (MQ=255)                                                                                                          gATAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTg                         <  2:1068218/140‑1 (MQ=255)                                                                                                           aTAATGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGc                        <  1:1232043/140‑1 (MQ=255)                                                                                                             aaTGCGAACAGCGCACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCg                      <  1:1431153/140‑1 (MQ=255)                                                                                                                        cgcACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTcagcagcag           >  1:1337301/1‑140 (MQ=255)                                                                                                                        cgcACGGCGTTAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATAAGCAACGGCTTGCCGTTcagcagcag           >  1:825710/1‑140 (MQ=255)                                                                                                                                 ttAAAGTCGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCAtt  >  2:365171/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGGTCAGACGATTCATTGGCACCATGCCGTGGGTTTCAATATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATT  >  NC_000913/365021‑365288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑