Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	595,224	G→A	P74S (CCG→TCG)	cusR ←	DNA‑binding transcriptional activator CusR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	595,224	0	G	A	100.0%	197.5 / NA	56	P74S (CCG→TCG)	cusR	DNA‑binding transcriptional activator CusR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/32);  total (24/32)

CGCCGCAGTAATGTGCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAGCCCGTT  >  NC_000913/595091‑595350                                                                                                                                      |                                                                                                                               cgccgcAGTAATGTGCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATccc                                                                                                                          >  1:1245273/1‑140 (MQ=255)         tAATGTGCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTgg                                                                                                                  <  1:672363/140‑1 (MQ=255)          aaTGTGCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 <  1:1436163/140‑1 (MQ=255)              tgCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCgg                                                                                                               >  2:84887/1‑138 (MQ=255)              tgCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCgg                                                                                                               <  1:84887/138‑1 (MQ=255)              tgCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAg                                                                                                             >  1:1452755/1‑140 (MQ=255)                cgcACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGTCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCg                                                                                                           >  1:1423334/1‑140 (MQ=255)                cgcACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCg                                                                                                           >  2:1467461/1‑140 (MQ=255)                 gcACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGt                                                                                                          >  1:1300584/1‑140 (MQ=255)                   aCCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTgg                                                                                                                  >  2:1149071/1‑130 (MQ=255)                   aCCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTgg                                                                                                                  <  1:1149071/130‑1 (MQ=255)                   aCCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 <  2:239826/131‑1 (MQ=255)                   aCCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 >  1:239826/1‑131 (MQ=255)                     ccGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 <  1:1471348/129‑1 (MQ=255)                     ccGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 >  2:394047/1‑129 (MQ=255)                     ccGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 >  2:1471348/1‑129 (MQ=255)                     ccGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGc                                                                                                                 <  1:394047/129‑1 (MQ=255)                     ccGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACa                                                                                                      >  1:292803/1‑140 (MQ=255)                         gcCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATgcg                                                                                                  <  2:777577/140‑1 (MQ=255)                         gcCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATgcg                                                                                                  <  1:173335/140‑1 (MQ=255)                             gcagTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACg                                                                                              <  1:1465619/140‑1 (MQ=255)                                                  gCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTcc                                                                         <  1:239802/140‑1 (MQ=255)                                                    ttCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCATGATATCCCAGCCGTTCACGTCCgg                                                                       <  2:185071/140‑1 (MQ=255)                                                    ttCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCgg                                                                       <  2:432451/140‑1 (MQ=255)                                                    ttCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCgg                                                                       <  2:1423334/140‑1 (MQ=255)                                                         cAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcagca                                                                  <  1:1467461/140‑1 (MQ=255)                                                         cAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGgcagca                                                                  <  2:1452755/140‑1 (MQ=255)                                                           ggTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCata                                                                <  2:1192170/140‑1 (MQ=255)                                                              aGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCataata                                                             >  2:1102323/1‑140 (MQ=255)                                                               gTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAatat                                                            <  1:201824/140‑1 (MQ=255)                                                               gTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAatat                                                            <  2:1245273/140‑1 (MQ=255)                                                                tCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATc                                                           >  2:599367/1‑140 (MQ=255)                                                                   tCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAg                                                        <  2:292803/140‑1 (MQ=255)                                                                   tCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAg                                                        <  1:599367/140‑1 (MQ=255)                                                                     tGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCg                                                          <  1:1061300/136‑1 (MQ=255)                                                                     tGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCg                                                          >  2:1061300/1‑136 (MQ=255)                                                                      gCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGAt                                                     >  2:1000127/1‑140 (MQ=255)                                                                       cccccAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGAtt                                                    >  1:936133/1‑140 (MQ=255)                                                                          ccAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATc                                                 <  1:1106492/140‑1 (MQ=255)                                                                                 cAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTa                                                   <  2:50109/131‑1 (MQ=255)                                                                                 cAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTa                                                   >  1:50109/1‑131 (MQ=255)                                                                                 cAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCAt                                          >  1:795306/1‑140 (MQ=255)                                                                                        ttGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCAcc                                   >  1:231450/1‑140 (MQ=255)                                                                                                   ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCa                        <  1:1281771/140‑1 (MQ=255)                                                                                                   ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCa                        <  1:753134/140‑1 (MQ=255)                                                                                                   ttCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCa                        <  2:795306/140‑1 (MQ=255)                                                                                                             ccAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAg                >  2:199880/1‑138 (MQ=255)                                                                                                             ccAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAg                <  1:31599/138‑1 (MQ=255)                                                                                                             ccAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAg                >  2:31599/1‑138 (MQ=255)                                                                                                             ccAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAg                <  1:199880/138‑1 (MQ=255)                                                                                                             ccAAGCGCGGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGcc              >  2:811278/1‑140 (MQ=255)                                                                                                                   gcgGTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAg        <  2:838199/140‑1 (MQ=255)                                                                                                                     ggTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAGcc      >  1:477056/1‑140 (MQ=255)                                                                                                                     ggTAAGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAGcc      >  1:111271/1‑140 (MQ=255)                                                                                                                         aGCAACAGAATCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAGCCCGtt  <  1:1000127/140‑1 (MQ=255)                                                                                                                                   tCGACATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAGCCCGtt  <  2:1297379/130‑1 (MQ=255)                                                                                                                                      |                                                                                                                               CGCCGCAGTAATGTGCGCACCCGCGCCAGCAGTTCAGCAAAAGCGAATGGCTTCACCAGGTAGTCATCTGCCCCCAACTCCAGCCCCTTGACGCGATGTTCAATGGTGCCAAGCGCGGTAAGCAACAGAATCGGCATCCCTTTATTGGCGGAGCGTAACATGCGCACGATATCCCAGCCGTTCACGTCCGGCAGCATAATATCGAGGATTATCAGATCATAATCACCGGTCATCGCCAGATGGTAGCCATTCAGCCCGTT  >  NC_000913/595091‑595350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑