Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	733,643	T→C	F1354S (TTC→TCC)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	733,643	0	T	C	100.0%	243.8 / NA	70	F1354S (TTC→TCC)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/38);  total (32/38)

AGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATTCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGATGTAAT  >  NC_000913/733506‑733777                                                                                                                                          |                                                                                                                                       aGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCca                                                                                                                                      >  2:632404/1‑140 (MQ=255)   gTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCcaca                                                                                                                                    <  1:1166017/140‑1 (MQ=255)   gTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCcaca                                                                                                                                    <  1:38632/140‑1 (MQ=255)   gTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCcaca                                                                                                                                    <  2:1146498/140‑1 (MQ=255)            gTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAAt                                                                                                                           >  1:184260/1‑140 (MQ=255)             tAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAAtt                                                                                                                          >  1:1048724/1‑140 (MQ=255)              aaTGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAAttt                                                                                                                         >  2:492636/1‑140 (MQ=255)                tGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAg                                                                                                                       <  2:1338464/140‑1 (MQ=255)                  aTACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAggg                                                                                                                     >  1:379409/1‑140 (MQ=255)                   tACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGc                                                                                                                    <  2:1256601/140‑1 (MQ=255)                   tACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGc                                                                                                                    >  1:640478/1‑140 (MQ=255)                            agCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTaaaa                                                                                                           <  1:492636/140‑1 (MQ=255)                            agCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTaaaa                                                                                                           <  1:909409/140‑1 (MQ=255)                             gctgctGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTaaaaa                                                                                                          <  2:788693/140‑1 (MQ=255)                                 ctgGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGc                                                                                                      >  1:297319/1‑140 (MQ=255)                                 ctgCCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGc                                                                                                      <  1:1235863/140‑1 (MQ=255)                                  tgGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGcc                                                                                                     <  2:297319/140‑1 (MQ=255)                                    gCCGTTGTGACCTCGTTGAAAGCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCaa                                                                                                   >  2:1047766/1‑140 (MQ=255)                                      cGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGa                                                                                                 <  2:1466493/140‑1 (MQ=255)                                       gTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGAc                                                                                                <  2:959204/140‑1 (MQ=255)                                       gTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGAc                                                                                                <  1:710638/140‑1 (MQ=255)                                          gtgACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTgg                                                                                             <  1:1307842/140‑1 (MQ=255)                                          gtgACCTCGTTGAAACCGATAATAGTTGAGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTgg                                                                                             <  1:871557/140‑1 (MQ=255)                                             aCCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCt                                                                                          >  1:820693/1‑140 (MQ=255)                                              ccTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTa                                                                                         >  2:385473/1‑140 (MQ=255)                                               cTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTaa                                                                                        >  1:1113681/1‑140 (MQ=255)                                               cTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTaa                                                                                        <  1:1047766/140‑1 (MQ=255)                                                  gTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGa                                                                                     <  2:1048724/140‑1 (MQ=255)                                                  gTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGa                                                                                     >  2:1122819/1‑140 (MQ=255)                                                  gTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGa                                                                                     <  1:996325/140‑1 (MQ=255)                                                            ataataGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGaa                                                                           <  2:581103/140‑1 (MQ=255)                                                                taGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACAt                                                                       <  1:822244/140‑1 (MQ=255)                                                                  gTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGt                                                                     <  2:1113681/140‑1 (MQ=255)                                                                   ttGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTa                                                                    >  2:786817/1‑140 (MQ=255)                                                                     gtgtgGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAg                                                                  >  1:253197/1‑140 (MQ=255)                                                                      tgtgGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAAc                                                                         <  1:1445138/132‑1 (MQ=255)                                                                      tgtgGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAAc                                                                         >  2:1445138/1‑132 (MQ=255)                                                                       gtgGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAACCTGCCAAGACTGGTCTAATGATTC‑TTAGAAACATGTAAGATg                                                               <  2:477532/140‑1 (MQ=255)                                                                           agaACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAg                                                            >  1:419095/1‑140 (MQ=255)                                                                            gaACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGt                                                           <  2:1289658/140‑1 (MQ=255)                                                                                  tttAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCg                                                     >  1:1376101/1‑140 (MQ=255)                                                                                       aaGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCGTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAAt                                                <  2:1015293/140‑1 (MQ=255)                                                                                            aaGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACAt                                           >  2:1239433/1‑140 (MQ=255)                                                                                            aaGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTACCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACAt                                           >  1:627186/1‑140 (MQ=255)                                                                                                        gTAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTttat                               <  2:419095/140‑1 (MQ=255)                                                                                                         tAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTTGACGTTttatt                              >  2:285040/1‑140 (MQ=255)                                                                                                         tAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGAc                                      <  1:726369/132‑1 (MQ=255)                                                                                                         tAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGAc                                      >  2:726369/1‑132 (MQ=255)                                                                                                         tAAGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTttatt                              >  2:1131377/1‑140 (MQ=255)                                                                                                          aaGGCCGCGTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTACCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTttatta                             <  2:627186/140‑1 (MQ=255)                                                                                                                gcgTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTg                       >  1:1245248/1‑140 (MQ=255)                                                                                                                  gTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTa                     >  1:557551/1‑140 (MQ=255)                                                                                                                  gTTATTCTGTATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTa                     <  1:1239433/140‑1 (MQ=255)                                                                                                                           tATTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGa            >  2:944927/1‑140 (MQ=255)                                                                                                                            aTTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAg           <  2:820693/140‑1 (MQ=255)                                                                                                                            aTTGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAg           <  1:944927/140‑1 (MQ=255)                                                                                                                              tGCCTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTg         <  1:1386061/140‑1 (MQ=255)                                                                                                                                ccTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCAc                >  1:1446621/1‑131 (MQ=255)                                                                                                                                ccTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCAc                <  2:1446621/131‑1 (MQ=255)                                                                                                                                ccTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAg           <  2:843432/136‑1 (MQ=255)                                                                                                                                ccTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAg           >  1:843432/1‑136 (MQ=255)                                                                                                                                ccTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGAt       >  1:600405/1‑140 (MQ=255)                                                                                                                                 cTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTTGACGTTTTATTAGATTTGTATTCACCGGAGTGATg      <  1:285040/140‑1 (MQ=255)                                                                                                                                 cTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGATg      <  1:385473/140‑1 (MQ=255)                                                                                                                                 cTGATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTAACCGGAGTGATg      >  2:983846/1‑140 (MQ=255)                                                                                                                                   gATATATCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGATGTa    <  2:521756/140‑1 (MQ=255)                                                                                                                                     tatatCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCAc                <  2:1008628/126‑1 (MQ=255)                                                                                                                                     tatatCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCAc                >  1:1008628/1‑126 (MQ=255)                                                                                                                                     tatatCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGATGTAAt  >  2:60834/1‑140 (MQ=255)                                                                                                                                     tatatCCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGATGTAAt  >  1:174281/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACCGATAATAGTTGTGTGGAGAACTGTTTAAAAGGGAAGTTTAAAGAGGTAAGGCCGCGTTATTCTGTATTGCCTGATATATTCACACCTATAAATTTAGGGCTATTTAAAAACTGCCAAGACTGGTCTAATGATTCTTTAGAAACATGTAAGATGAAGTGCTCCGGAAATAACATTGGACGTTTTATTAGATTTGTATTCACCGGAGTGATGTAAT  >  NC_000913/733506‑733777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑