Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	62,358	C→T	A303T (GCA→ACA)	rapA ←	RNA polymerase‑binding ATPase and RNAP recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	62,358	0	C	T	100.0%	281.4 / NA	80	A303T (GCA→ACA)	rapA	RNA polymerase‑binding ATPase and RNAP recycling factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/42);  total (38/42)

TTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCA  >  NC_000913/62222‑62491                                                                                                                                         |                                                                                                                                      tttttCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAg                                                                                                                                    <  1:857070/140‑1 (MQ=255)        gCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCa                                                                                                                             >  1:1065404/1‑140 (MQ=255)        gCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCa                                                                                                                             <  1:161423/140‑1 (MQ=255)         ctctTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCaa                                                                                                                            >  1:1257480/1‑140 (MQ=255)         ctctTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCaa                                                                                                                            <  2:486787/140‑1 (MQ=255)             tCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGc                                                                                                                        <  1:311289/140‑1 (MQ=255)             tCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGc                                                                                                                        <  2:426359/140‑1 (MQ=255)             tCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGc                                                                                                                        <  1:1184116/140‑1 (MQ=255)                aCGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTg                                                                                                                     <  1:147452/140‑1 (MQ=255)                aCGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTg                                                                                                                     <  2:1065404/140‑1 (MQ=255)                   aaCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGata                                                                                                                  >  1:1089771/1‑140 (MQ=255)                      tGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTc                                                                                                               >  2:1030933/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGc                                                                                                                        <  2:1278289/129‑1 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGc                                                                                                                        >  1:1278289/1‑129 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  1:447956/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  2:156353/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  2:318095/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  2:452635/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  2:787773/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  2:931211/1‑140 (MQ=255)                        cgcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCAc                                                                                                             >  1:829233/1‑140 (MQ=255)                         gcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAAt                                                                                                                           <  2:1054658/125‑1 (MQ=255)                         gcAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAAt                                                                                                                           >  1:1054658/1‑125 (MQ=255)                                 gTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGgcg                                                                                                    <  2:447956/140‑1 (MQ=255)                                 gTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGgcg                                                                                                    <  1:1030933/140‑1 (MQ=255)                                 gTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCAGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGgcg                                                                                                    <  1:471043/140‑1 (MQ=255)                                  tGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGTTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGgcgc                                                                                                   >  2:798417/1‑140 (MQ=255)                                  tGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGgcgc                                                                                                   >  1:1259027/1‑140 (MQ=255)                                    gAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCAt                                                                                                 <  2:829233/140‑1 (MQ=255)                                       aCGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCtt                                                                                              <  2:1019903/140‑1 (MQ=255)                                         ggTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCg                                                                                            >  1:445850/1‑140 (MQ=255)                                         ggTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCg                                                                                            >  1:1379860/1‑140 (MQ=255)                                           ttCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCt                                                                                          <  1:36822/140‑1 (MQ=255)                                           ttCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCt                                                                                          >  2:1473305/1‑140 (MQ=255)                                           ttCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCt                                                                                          <  1:156353/140‑1 (MQ=255)                                            tCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTc                                                                                         <  2:1089771/140‑1 (MQ=255)                                             cGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTcc                                                                                        >  1:623731/1‑140 (MQ=255)                                               ggTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCcac                                                                                      >  1:1032034/1‑140 (MQ=255)                                                gTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCcaca                                                                                     <  2:628743/140‑1 (MQ=255)                                                gTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCcaca                                                                                     <  2:1085638/140‑1 (MQ=255)                                                   cagcagACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCa                                                                                  >  1:578904/1‑140 (MQ=255)                                                     gcagacgcagacGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAgg                                                                                >  1:1313465/1‑140 (MQ=255)                                                              gacgGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCt                                                                       <  2:863506/140‑1 (MQ=255)                                                               acgGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACGCCAAGTGATGCGAtt                                                                      <  2:1032034/140‑1 (MQ=255)                                                                  ggCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCAt                                                                   <  2:445850/140‑1 (MQ=255)                                                                   gCGAAGTAGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATc                                                                  >  2:691834/1‑140 (MQ=255)                                                                       aGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGacc                                                              >  2:688476/1‑140 (MQ=255)                                                                       aGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGacc                                                              >  1:238411/1‑140 (MQ=255)                                                                       aGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGacc                                                              <  2:238411/140‑1 (MQ=255)                                                                            cTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACcag                                                         <  2:881097/140‑1 (MQ=255)                                                                             tttCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACcagc                                                        <  1:691834/140‑1 (MQ=255)                                                                             tttCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACcagc                                                        <  2:718196/140‑1 (MQ=255)                                                                             tttCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACcagc                                                        >  1:681419/1‑140 (MQ=255)                                                                              ttCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACcagca                                                       >  1:1073470/1‑140 (MQ=255)                                                                                ccATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAgg                                                     <  1:1157668/140‑1 (MQ=255)                                                                                 cATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGt                                                    >  2:370724/1‑140 (MQ=255)                                                                                   tCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTcc                                                  >  2:1122389/1‑140 (MQ=255)                                                                                     cccAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCa                                                >  2:745337/1‑140 (MQ=255)                                                                                       cAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCAtt                                              <  2:1073470/140‑1 (MQ=255)                                                                                          cTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCgg                                           <  1:452635/140‑1 (MQ=255)                                                                                          cTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCgg                                           <  2:1116334/140‑1 (MQ=255)                                                                                          cTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCgg                                           <  2:681419/140‑1 (MQ=255)                                                                                            gTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCt                                         <  1:318095/140‑1 (MQ=255)                                                                                               ccGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTca                                      <  2:1257480/140‑1 (MQ=255)                                                                                                 ggggTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTcaca                                    <  2:1313465/140‑1 (MQ=255)                                                                                                  gggTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACag                                   >  1:324932/1‑140 (MQ=255)                                                                                                  gggTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACag                                   >  1:63667/1‑140 (MQ=255)                                                                                                       gcgGTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATg                              >  1:926983/1‑140 (MQ=255)                                                                                                          gTCAGCAGCAGAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTc                           <  2:1379860/140‑1 (MQ=255)                                                                                                             agcagcagAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTcc                          >  2:653652/1‑138 (MQ=255)                                                                                                             agcagcagAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTcc                          <  1:653652/138‑1 (MQ=255)                                                                                                              gcagcagAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAgg                       <  1:1473305/140‑1 (MQ=255)                                                                                                               cagcagAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGgc                      <  1:688476/140‑1 (MQ=255)                                                                                                                agcagAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGgcg                     <  2:578904/140‑1 (MQ=255)                                                                                                                  cagAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCt                   <  1:370724/140‑1 (MQ=255)                                                                                                                   agAACGCCCGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTg                  <  2:1259027/140‑1 (MQ=255)                                                                                                                          ccGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTa           >  1:231217/1‑140 (MQ=255)                                                                                                                          ccGGCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTa           >  2:964102/1‑140 (MQ=255)                                                                                                                            ggCACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTacg         >  2:1025298/1‑140 (MQ=255)                                                                                                                              cACGTGCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTacgac       <  1:1122389/140‑1 (MQ=255)                                                                                                                                   gCTCTGTCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCa  >  1:1062473/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAATGGCCTGATATTCACGGCTTGGCGCATCTTCGCTCCACACCAGGTGATGCGCTTCATCGACCACCAGCAGGTCCCATTCGGCTTCACAGAGATGTTCCAGGCGCTGTTTGCTACGACGGGCA  >  NC_000913/62222‑62491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑