Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,580	C→T	E134K (GAA→AAA)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,580	0	C	T	98.9%	323.0 / NA	91	E134K (GAA→AAA)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/40);  minor base A (0/1);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTT  >  NC_000913/789453‑789715                                                                                                                                |                                                                                                                                        aGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGga                                                                                                                             >  1:1264668/1‑140 (MQ=255)       tCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACt                                                                                                                       <  2:928604/140‑1 (MQ=255)       tCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGAAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACt                                                                                                                       <  1:649865/140‑1 (MQ=255)           tGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAAc                                                                                                                   >  2:774856/1‑140 (MQ=255)                ccGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGc                                                                                                              >  1:272392/1‑140 (MQ=255)                ccGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGc                                                                                                              >  2:1425434/1‑140 (MQ=255)                ccGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGc                                                                                                              >  2:1253178/1‑140 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCg                                                                                                                >  1:84633/1‑137 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCg                                                                                                                <  2:84633/137‑1 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCa                                                                                                             >  2:1353357/1‑140 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCa                                                                                                             <  1:998094/140‑1 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCa                                                                                                             >  1:847278/1‑140 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCa                                                                                                             <  1:1253178/140‑1 (MQ=255)                 cGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCa                                                                                                             >  2:787226/1‑140 (MQ=255)                  gCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCAc                                                                                                            >  1:52865/1‑140 (MQ=255)                  gCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCAc                                                                                                            >  1:985996/1‑140 (MQ=255)                      ttACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTg                                                                                                        >  2:522285/1‑140 (MQ=255)                        aCAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCg                                                                                                      >  2:512727/1‑140 (MQ=255)                        aCAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCg                                                                                                      >  2:1207339/1‑140 (MQ=255)                          aGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGc                                                                                                    >  2:573343/1‑140 (MQ=255)                             cTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGcaca                                                                                                 >  2:582031/1‑140 (MQ=255)                             cTACAAACTGGTTTTCTGCTTCCTGACCGCTAAGCGCGTTTTGTGCGCCGTCCAGCGGCAGTTGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGtaca                                                                                                 >  2:346407/1‑140 (MQ=255)                              tACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACAt                                                                                                >  2:1172153/1‑140 (MQ=255)                                cAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTg                                                                                              >  1:873356/1‑140 (MQ=255)                                cAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTg                                                                                              >  2:1016111/1‑140 (MQ=255)                                cAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTg                                                                                              >  1:888576/1‑140 (MQ=255)                                 aaaCTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGc                                                                                             <  2:873356/140‑1 (MQ=255)                                  aaCTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGcc                                                                                            <  1:512727/140‑1 (MQ=255)                                  aaCTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGcc                                                                                            <  1:774856/140‑1 (MQ=255)                                  aaCTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGcc                                                                                            <  2:928717/140‑1 (MQ=255)                                   aCTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCg                                                                                           >  2:1453921/1‑140 (MQ=255)                                     tGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCt                                                                                         <  2:882832/140‑1 (MQ=255)                                      ggTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTg                                                                                        <  1:522285/140‑1 (MQ=255)                                      ggTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTg                                                                                        <  1:1172153/140‑1 (MQ=255)                                       gTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGa                                                                                       <  1:573343/140‑1 (MQ=255)                                       gTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGa                                                                                       <  1:1233656/140‑1 (MQ=255)                                       gTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGa                                                                                       <  1:1214266/140‑1 (MQ=255)                                       gTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGa                                                                                       >  1:977183/1‑140 (MQ=255)                                         tttCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATc                                                                                     <  2:242309/140‑1 (MQ=255)                                           tCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCAc                                                                                   <  2:921309/140‑1 (MQ=255)                                               cTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATg                                                                               <  1:1353357/140‑1 (MQ=255)                                                      aCCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCAcgc                                                                        >  1:1010871/1‑140 (MQ=255)                                                         gTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCAcgccgc                                                                     >  1:564757/1‑140 (MQ=255)                                                         gTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCAACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCAcgccgc                                                                     >  1:266178/1‑140 (MQ=255)                                                          ttAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACgccgcc                                                                    >  2:386738/1‑140 (MQ=255)                                                           tAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGccgccg                                                                   >  2:287687/1‑140 (MQ=255)                                                           tAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGccgccg                                                                   <  1:1425434/140‑1 (MQ=255)                                                            aaGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGa                                                                  >  2:1296909/1‑140 (MQ=255)                                                               cgcgATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGc                                                               <  1:46235/140‑1 (MQ=255)                                                               cgcgATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGc                                                               <  1:287687/140‑1 (MQ=255)                                                               cgcgATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGc                                                               <  2:1264668/140‑1 (MQ=255)                                                                  gATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCtgt                                                            >  1:536075/1‑140 (MQ=255)                                                                  gATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCtgt                                                            >  2:767744/1‑140 (MQ=255)                                                                    tttGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTGACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCtgttg                                                          >  2:1141305/1‑140 (MQ=255)                                                                    tttGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCtgttg                                                          >  1:301758/1‑140 (MQ=255)                                                                         gcgcCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACg                                                     <  2:301758/140‑1 (MQ=255)                                                                                       cAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGtt                                       >  1:685593/1‑140 (MQ=255)                                                                                         gatgatAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAg                                                  <  1:1083242/127‑1 (MQ=255)                                                                                         gatgatAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAg                                                  >  2:1083242/1‑127 (MQ=255)                                                                                         gatgatAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTc                                     >  1:111255/1‑140 (MQ=255)                                                                                         gatgatAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTc                                     >  2:203943/1‑140 (MQ=255)                                                                                         gatgatAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTc                                     >  1:601220/1‑140 (MQ=255)                                                                                              tAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCa                                             >  2:726095/1‑127 (MQ=255)                                                                                              tAAAGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCa                                             <  1:726095/127‑1 (MQ=255)                                                                                                aaGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgc                              <  2:536075/140‑1 (MQ=255)                                                                                                 aGCTGCTGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgcc                             <  1:787226/140‑1 (MQ=255)                                                                                                  gctgctGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTGACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgcca                            <  1:1141305/140‑1 (MQ=255)                                                                                                  gctgctGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgcca                            <  1:412850/140‑1 (MQ=255)                                                                                                  gctgctGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgcca                            <  2:977183/140‑1 (MQ=255)                                                                                                  gctgctGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgcca                            <  1:386738/140‑1 (MQ=255)                                                                                                  gctgctGCAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCAccacgcca                            <  2:111255/140‑1 (MQ=255)                                                                                                     gctgcAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGa                         >  2:124719/1‑140 (MQ=255)                                                                                                      ctgcAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCTACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGaa                        >  1:289280/1‑140 (MQ=255)                                                                                                      ctgcAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGaa                        >  2:359989/1‑140 (MQ=255)                                                                                                      ctgcAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGaa                        <  2:888576/140‑1 (MQ=255)                                                                                                      ctgcAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGaa                        <  2:847278/140‑1 (MQ=255)                                                                                                      ctgcAATACGGTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGaa                        >  2:337044/1‑140 (MQ=255)                                                                                                      ctgcAATACGGTTCCGACCGCGACATTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGaa                        >  1:1126249/1‑140 (MQ=255)                                                                                                       tgcAATACGGTTCCGACCGCGACTTACAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAAc                       <  2:685593/140‑1 (MQ=255)                                                                                                               ggTTCCGCCCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAg               <  2:272392/140‑1 (MQ=255)                                                                                                                gTTCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGc              <  1:1207339/140‑1 (MQ=255)                                                                                                                 ttCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGcc             >  1:1426568/1‑140 (MQ=255)                                                                                                                 ttCCGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGcc             >  2:1093661/1‑140 (MQ=255)                                                                                                                   ccGACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCa           >  1:585614/1‑140 (MQ=255)                                                                                                                     gACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCAtt         <  2:1010871/140‑1 (MQ=255)                                                                                                                     gACCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCAtt         <  1:124719/140‑1 (MQ=255)                                                                                                                      aCCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTg        >  2:769355/1‑140 (MQ=255)                                                                                                                      aCCGCGACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTg        >  2:32994/1‑140 (MQ=255)                                                                                                                           gACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGtt                 <  2:1308857/126‑1 (MQ=255)                                                                                                                           gACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGtt                 >  1:1308857/1‑126 (MQ=255)                                                                                                                           gACTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGt   >  2:470564/1‑140 (MQ=255)                                                                                                                            aCTTTCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGtt  <  1:1453921/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                        AGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACTTAACCCGGCACCCTGCGGCACATTGCCGCTGATCACCATGTCCACGCCGCCGAAGCTGTTGTTACGCAGTTGCAGATGTTTCACCACGCCACGAACGTAGTTAGCCCATTGATAGTT  >  NC_000913/789453‑789715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑