Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,985	T→C	intergenic (+38/‑541)	bioD → / → uvrB	dethiobiotin synthetase/excision nuclease subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,985	0	T	C	100.0%	181.0 / NA	53	intergenic (+38/‑541)	bioD/uvrB	dethiobiotin synthetase/excision nuclease subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

ATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTC  >  NC_000913/812852‑813119                                                                                                                                      |                                                                                                                                        aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGa                                                                                                                                   <  2:528208/140‑1 (MQ=255) aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGa                                                                                                                                   >  2:1293012/1‑140 (MQ=255) aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGa                                                                                                                                   >  2:502926/1‑140 (MQ=255)     ttCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGa                                                                                                                               >  1:712783/1‑140 (MQ=255)      tCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGAc                                                                                                                              <  1:1131409/140‑1 (MQ=255)           cgccgcTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACt                                                                                                                             <  2:1205890/136‑1 (MQ=255)           cgccgcTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACt                                                                                                                             >  1:1205890/1‑136 (MQ=255)               gctgctGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTa                                                                                                                     <  1:147809/140‑1 (MQ=255)                ctgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTaa                                                                                                                    >  2:264432/1‑140 (MQ=255)                       gagagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTa                                                                                                            <  2:409880/140‑1 (MQ=255)                           gaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAAtt                                                                                                        >  2:1426941/1‑140 (MQ=255)                                 cTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTaaaa                                                                                                  >  2:318629/1‑140 (MQ=255)                                    gCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAaataa                                                                                               >  2:517436/1‑140 (MQ=255)                                      ttGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAAtt                                                                                                             >  2:1093645/1‑124 (MQ=255)                                      ttGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAAtt                                                                                                             <  1:1093645/124‑1 (MQ=255)                                           gAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGaa                                                                                        <  1:1287892/140‑1 (MQ=255)                                                 ccAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAAt                                                                                  <  2:712783/140‑1 (MQ=255)                                                       aaTGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACt                                                                            <  1:1293012/140‑1 (MQ=255)                                                        aTGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACtt                                                                           <  2:112676/140‑1 (MQ=255)                                                                      aaGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                                                             <  2:1410963/140‑1 (MQ=255)                                                                           cATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAAtg                                                        <  1:264432/140‑1 (MQ=255)                                                                                   ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAtt                                                >  2:1354059/1‑140 (MQ=255)                                                                                   ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAtt                                                >  2:1035990/1‑140 (MQ=255)                                                                                    tgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTg                                               >  2:1267170/1‑140 (MQ=255)                                                                                     gccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAACCCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTg                                               >  2:1275111/1‑140 (MQ=255)                                                                                     gccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGc                                              >  1:1033858/1‑140 (MQ=255)                                                                                      ccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                                                             <  2:821265/124‑1 (MQ=255)                                                                                      ccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                                                             >  1:821265/1‑124 (MQ=255)                                                                                      ccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGcc                                             >  1:597818/1‑140 (MQ=255)                                                                                      ccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGcc                                             <  2:240836/140‑1 (MQ=255)                                                                                          gttgtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAgg                                         >  2:625515/1‑140 (MQ=255)                                                                                           ttgtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAggc                                        >  1:947519/1‑140 (MQ=255)                                                                                             gtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCCCATGCGGCGAATGTGGGAATTGCCCAggcgg                                      <  2:451194/140‑1 (MQ=255)                                                                                                     tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAt                              >  2:1051393/1‑140 (MQ=255)                                                                                                     tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAt                              >  1:1376341/1‑140 (MQ=255)                                                                                                     tCTGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAt                              >  2:336994/1‑140 (MQ=255)                                                                                                       tGTATTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAg                            <  2:213441/140‑1 (MQ=255)                                                                                                          aTTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAgggg                         <  2:947519/140‑1 (MQ=255)                                                                                                          aTTTGGTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAgggg                         <  1:336994/140‑1 (MQ=255)                                                                                                               gTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGga                    >  2:1206719/1‑140 (MQ=255)                                                                                                               gTTAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGga                    >  2:1330894/1‑140 (MQ=255)                                                                                                                 tAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGgaga                  <  2:406235/140‑1 (MQ=255)                                                                                                                 tAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGgaga                  <  1:318629/140‑1 (MQ=255)                                                                                                                 tAAATTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACCTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGgaga                  >  1:695909/1‑140 (MQ=255)                                                                                                                  aaaTTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAACCCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGgaga                  <  1:1275111/140‑1 (MQ=255)                                                                                                                   aaTTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACa                <  1:1051393/140‑1 (MQ=255)                                                                                                                   aaTTGCGAGCGAGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACa                >  2:112072/1‑140 (MQ=255)                                                                                                                       gcgagcgaGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTa            <  1:502926/140‑1 (MQ=255)                                                                                                                       gcgagcgaGATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTa            <  2:1033858/140‑1 (MQ=255)                                                                                                                            cgagATCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCAc       <  2:597818/140‑1 (MQ=255)                                                                                                                              agaTCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTa     <  1:1018251/140‑1 (MQ=255)                                                                                                                                 tCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTc  <  2:205000/140‑1 (MQ=255)                                                                                                                                 tCGCGCCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTc  <  2:1376341/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        ATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAA‑CCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGATAGGGGCTGGAGACAGTTATCCACTATTC  >  NC_000913/812852‑813119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑