Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 899,900 C→T A226T (GCA→ACA)  artJ ← L‑arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913899,9000CT100.0% 237.5 / NA 68A226T (GCA→ACA) artJL‑arginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/29);  total (39/29)

GTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGCTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGCCAT  >  NC_000913/899765‑900036
                                                                                                                                       |                                                                                                                                        
gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGcc                                                                                                                                      >  1:924298/1‑140 (MQ=255)
 tAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCa                                                                                                                                     <  2:526047/140‑1 (MQ=255)
    gCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAgcg                                                                                                                                  <  2:949502/140‑1 (MQ=255)
    gCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAgcg                                                                                                                                  <  1:1200230/140‑1 (MQ=255)
    gCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAgcg                                                                                                                                  >  2:894440/1‑140 (MQ=255)
         aTAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             >  1:1383431/1‑140 (MQ=255)
         aTAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             >  1:452954/1‑140 (MQ=255)
         aTAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             >  2:356313/1‑140 (MQ=255)
         aTAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             >  1:1372226/1‑140 (MQ=255)
         aTAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             >  2:51870/1‑140 (MQ=255)
            aGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTc                                                                                                                          <  2:1253699/140‑1 (MQ=255)
              cGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAg                                                                                                                        >  2:1049828/1‑140 (MQ=255)
                  gcgcATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGtttt                                                                                                                    <  2:1383431/140‑1 (MQ=255)
                  gcgcATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAACTACTGCCAGCTTTAATTGTTGCCAGCGCGTTATTCAGtttt                                                                                                                    <  2:364818/140‑1 (MQ=255)
                   cgcATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             <  2:1411700/130‑1 (MQ=255)
                   cgcATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTa                                                                                                                             >  1:1411700/1‑130 (MQ=255)
                   cgcATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGttttt                                                                                                                   >  2:107639/1‑140 (MQ=255)
                   cgcATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGttttt                                                                                                                   >  1:346696/1‑140 (MQ=255)
                        cAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCcagc                                                                                                              >  2:835816/1‑140 (MQ=255)
                         aGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCcagca                                                                                                             <  1:835816/140‑1 (MQ=255)
                           gCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAgg                                                                                                           <  1:1049828/140‑1 (MQ=255)
                            cAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAggg                                                                                                          <  2:118800/140‑1 (MQ=255)
                                         gTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCgg                                                                                             <  2:1260317/140‑1 (MQ=255)
                                            atcaGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACg                                                                                          <  1:1148057/140‑1 (MQ=255)
                                              caGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTa                                                                                        <  2:924298/140‑1 (MQ=255)
                                               aGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTa                                                                                        >  1:148722/1‑139 (MQ=255)
                                               aGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTa                                                                                        <  2:148722/139‑1 (MQ=255)
                                                 tctcAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAg                                                                                     >  2:729924/1‑140 (MQ=255)
                                                   tcAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCg                                                                                   <  1:356313/140‑1 (MQ=255)
                                                   tcAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCg                                                                                   <  1:1105502/140‑1 (MQ=255)
                                                           cgGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAg                                                                           >  1:1004953/1‑140 (MQ=255)
                                                           cgGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAg                                                                           >  2:375264/1‑140 (MQ=255)
                                                           cgGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAg                                                                           >  2:510614/1‑140 (MQ=255)
                                                           cgGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAg                                                                           >  2:955758/1‑140 (MQ=255)
                                                           cgGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGAGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGACCAg                                                                           >  1:737507/1‑140 (MQ=255)
                                                            ggTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAgg                                                                          <  2:346696/140‑1 (MQ=255)
                                                              ttGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGcc                                                                        <  2:1372226/140‑1 (MQ=255)
                                                                      ttCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCaa                                                                >  1:494081/1‑140 (MQ=255)
                                                                       tCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCaaa                                                               <  1:107639/140‑1 (MQ=255)
                                                                        cTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCaaaa                                                              >  1:235051/1‑140 (MQ=255)
                                                                                tACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCgg                                                      >  2:420836/1‑140 (MQ=255)
                                                                                 aCTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGa                                                     >  2:1043094/1‑140 (MQ=255)
                                                                                 aCTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGa                                                     >  2:388263/1‑140 (MQ=255)
                                                                                 aCTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGa                                                     >  1:1461856/1‑140 (MQ=255)
                                                                                 aCTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGa                                                     >  1:132841/1‑140 (MQ=255)
                                                                                  cTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTGCCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGAt                                                    <  2:850222/140‑1 (MQ=255)
                                                                                  cTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGAt                                                    <  1:1192880/140‑1 (MQ=255)
                                                                                   tgtgGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATc                                                   >  1:1357537/1‑140 (MQ=255)
                                                                                       ggAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTc                                               <  1:955758/140‑1 (MQ=255)
                                                                                       ggAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTc                                               >  2:1316683/1‑140 (MQ=255)
                                                                                         aaCCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCAc                                             >  1:620092/1‑140 (MQ=255)
                                                                                          aCCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACt                                            >  1:1337543/1‑140 (MQ=255)
                                                                                          aCCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACt                                            >  1:1098151/1‑140 (MQ=255)
                                                                                            cACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACttt                                          <  2:494081/140‑1 (MQ=255)
                                                                                               tGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTATTTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTtctc                                       >  1:1438538/1‑140 (MQ=255)
                                                                                               tGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTtctc                                       >  1:707369/1‑140 (MQ=255)
                                                                                               tGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTtctc                                       >  2:343483/1‑140 (MQ=255)
                                                                                                ggTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCa                                      <  2:235051/140‑1 (MQ=255)
                                                                                                 gTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAg                                     >  2:1467715/1‑140 (MQ=255)
                                                                                                 gTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAg                                     >  1:1346796/1‑140 (MQ=255)
                                                                                                  tCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACt                                            <  1:159157/132‑1 (MQ=255)
                                                                                                  tCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACt                                            >  2:159157/1‑132 (MQ=255)
                                                                                                   cACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTa                                   <  2:707369/140‑1 (MQ=255)
                                                                                                   cACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTa                                   >  1:1342727/1‑140 (MQ=255)
                                                                                                              tGATAAGTACCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAg                        <  1:1467715/140‑1 (MQ=255)
                                                                                                                      aCCGTCAGCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGAt                <  2:1098151/140‑1 (MQ=255)
                                                                                                                             gCTTTAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGtttt         <  1:420836/140‑1 (MQ=255)
                                                                                                                                 tAATTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGc     >  1:1283589/1‑140 (MQ=255)
                                                                                                                                  atttgttgCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGcc    <  2:1438538/138‑1 (MQ=255)
                                                                                                                                  aaTTGTTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGcc    <  1:375264/140‑1 (MQ=255)
                                                                                                                                    ttgttgCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGGCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGCCAt  <  2:975979/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGCTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGCCAT  >  NC_000913/899765‑900036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑