Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	989,615	A→G	Y248H (TAT→CAT)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,615	0	A	G	100.0%	300.7 / NA	86	Y248H (TAT→CAT)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/43);  total (43/43)

CCCATTCAACCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTA  >  NC_000913/989486‑989747                                                                                                                                  |                                                                                                                                     cccATTCAACCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTccagc                                                                                                                            >  1:1018897/1‑140 (MQ=255) cccATTCAACCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTccagc                                                                                                                            >  2:824621/1‑140 (MQ=255)  ccATTCAACCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTccagcc                                                                                                                           <  1:749281/140‑1 (MQ=255)    aTTCAACCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTccagccag                                                                                                                         <  2:1460455/140‑1 (MQ=255)         aCCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGgcagc                                                                                                                    <  1:1396494/140‑1 (MQ=255)             ggTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGgca                                                                                                                      <  1:180960/134‑1 (MQ=255)             ggTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGgca                                                                                                                      >  2:180960/1‑134 (MQ=255)             ggTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGt                                                                                                                >  1:559103/1‑140 (MQ=255)              gTCGCCATAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTg                                                                                                               >  1:805065/1‑140 (MQ=255)               tCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTccagc                                                                                                                            >  2:1132735/1‑126 (MQ=255)               tCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTccagc                                                                                                                            <  1:1132735/126‑1 (MQ=255)               tCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGc                                                                                                              >  2:555396/1‑140 (MQ=255)                cGCCATAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCa                                                                                                             <  2:805065/140‑1 (MQ=255)                       gTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCgct                                                                                                      <  1:793138/140‑1 (MQ=255)                            gACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCgctggctg                                                                                                 <  1:1392833/140‑1 (MQ=255)                             aCGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGt                                                                                                >  2:983254/1‑140 (MQ=255)                             aCGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGt                                                                                                <  1:215119/140‑1 (MQ=255)                                cAGGCCCTGATACCGACTAGCGAACTCGACACGGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTgg                                                                                             >  1:992709/1‑140 (MQ=255)                                 aGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGc                                                                                            <  2:233378/140‑1 (MQ=255)                                 aGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGc                                                                                            >  1:499006/1‑140 (MQ=255)                                  ggCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCt                                                                                           >  2:1165831/1‑140 (MQ=255)                                  ggCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGCCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCt                                                                                           >  1:396094/1‑140 (MQ=255)                                         aTACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCg                                                                                    <  1:788342/140‑1 (MQ=255)                                           aCCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCggg                                                                                  <  1:1322457/140‑1 (MQ=255)                                           aCCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCggg                                                                                  <  2:357701/140‑1 (MQ=255)                                           aCCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCggg                                                                                  <  1:824621/140‑1 (MQ=255)                                            ccGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGc                                                                                 <  1:983254/140‑1 (MQ=255)                                               aCTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGa                                                                              >  2:1274306/1‑140 (MQ=255)                                                   gCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTg                                                                          >  2:213678/1‑140 (MQ=255)                                                        cTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGTAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGAt                                                                     <  2:259338/140‑1 (MQ=255)                                                        cTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGAt                                                                     <  2:1480059/140‑1 (MQ=255)                                                        cTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGAt                                                                     <  2:1018897/140‑1 (MQ=255)                                                        cTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGAt                                                                     <  2:1166472/140‑1 (MQ=255)                                                        cTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGAt                                                                     <  2:750546/140‑1 (MQ=255)                                                           gACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGACAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCggg                                                                                  <  2:401303/124‑1 (MQ=255)                                                           gACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGACAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCggg                                                                                  >  1:401303/1‑124 (MQ=255)                                                             cacCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGcc                                                                >  2:1131533/1‑140 (MQ=255)                                                              acCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCt                                                               >  1:370825/1‑140 (MQ=255)                                                                cGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTgg                                                             <  1:1131533/140‑1 (MQ=255)                                                                  aaaTCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGaa                                                           <  1:555396/140‑1 (MQ=255)                                                                   aaTCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAAc                                                          >  2:178906/1‑140 (MQ=255)                                                                       aCGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAAcc                                                         <  2:602055/137‑1 (MQ=255)                                                                       aCGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAAcc                                                         >  1:602055/1‑137 (MQ=255)                                                                        cGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTc                                                     <  1:1316750/140‑1 (MQ=255)                                                                           aGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCgtg                                                  <  1:1165831/140‑1 (MQ=255)                                                                             gAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGtgtg                                                <  1:1274306/140‑1 (MQ=255)                                                                              aaaGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGc                                               <  1:270938/140‑1 (MQ=255)                                                                                   aTCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCtgtg                                          >  1:1058628/1‑140 (MQ=255)                                                                                     ccATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGtt                                        >  1:166048/1‑140 (MQ=255)                                                                                       aTAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTcc                                      >  2:677577/1‑140 (MQ=255)                                                                                       aTAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTcc                                      >  1:432639/1‑140 (MQ=255)                                                                                       aTAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTcc                                      >  1:1118510/1‑140 (MQ=255)                                                                                             aTGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCGTGTGTTCCCATCgg                                >  2:905715/1‑140 (MQ=255)                                                                                             aTGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCgg                                >  1:1371614/1‑140 (MQ=255)                                                                                              tGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGc                               >  1:666192/1‑140 (MQ=255)                                                                                              tGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCAATCGGc                               >  1:849713/1‑140 (MQ=255)                                                                                               gCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCg                              <  1:905715/140‑1 (MQ=255)                                                                                                  gTCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTGCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGAGCCTGGATCCATTTGTGTGCCTGTGTTCCCATCGGCGTGa                           >  2:1306595/1‑140 (MQ=255)                                                                                                   tCGGTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAg                          >  2:602630/1‑140 (MQ=255)                                                                                                     ggTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAggg                        >  1:1184117/1‑140 (MQ=255)                                                                                                     ggTTAATGCAATGCCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAggg                        >  1:641580/1‑140 (MQ=255)                                                                                                            gcaatgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCg                 <  2:992709/140‑1 (MQ=255)                                                                                                             caatgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCGAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGa                >  2:823381/1‑140 (MQ=255)                                                                                                             caatgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGa                >  2:87824/1‑140 (MQ=255)                                                                                                             caatgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGa                <  1:903452/140‑1 (MQ=255)                                                                                                             caatgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGa                <  2:370825/140‑1 (MQ=255)                                                                                                               atgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcg              >  1:332243/1‑140 (MQ=255)                                                                                                               atgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcg              <  2:432639/140‑1 (MQ=255)                                                                                                                tgcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgc             >  1:6841/1‑140 (MQ=255)                                                                                                                 gcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGTTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            >  1:526315/1‑140 (MQ=255)                                                                                                                 gcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            <  2:1058628/140‑1 (MQ=255)                                                                                                                 gcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            >  1:1118432/1‑140 (MQ=255)                                                                                                                 gcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            >  1:912839/1‑140 (MQ=255)                                                                                                                 gcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            <  2:1371614/140‑1 (MQ=255)                                                                                                                 gcCAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTCCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            >  1:1137072/1‑140 (MQ=255)                                                                                                                  ccAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACgcgc           <  2:499006/140‑1 (MQ=255)                                                                                                                  ccAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACgcgc           <  2:1184117/140‑1 (MQ=255)                                                                                                                   cAAGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGcc          <  1:1306595/140‑1 (MQ=255)                                                                                                                    aaGTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCa         <  1:677577/140‑1 (MQ=255)                                                                                                                      gTTGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGa       <  1:1424639/140‑1 (MQ=255)                                                                                                                        tGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATc     <  1:4731/140‑1 (MQ=255)                                                                                                                        tGGTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATc     <  1:87824/140‑1 (MQ=255)                                                                                                                          gTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGt   >  1:505369/1‑140 (MQ=255)                                                                                                                          gTCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGt   >  2:1472061/1‑140 (MQ=255)                                                                                                                           tCGGGATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTa  >  1:365306/1‑140 (MQ=255)                                                                                                                               gATGCTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGAcgcg            <  2:1395580/126‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CCCATTCAACCGGGTCGCCAGAGTCATGACGCAGGCCCTGATACCGACTAGCGAACTCGACACCGAAATCACGCAGGAAAGCATCCATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTA  >  NC_000913/989486‑989747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑