Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	999,141	A→G	T218A (ACC→GCC)	elfD →	putative fimbrial chaperone ElfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	999,141	0	A	G	100.0%	268.6 / NA	77	T218A (ACC→GCC)	elfD	putative fimbrial chaperone ElfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/41);  total (36/41)

ACCCAACACCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATC  >  NC_000913/999004‑999268                                                                                                                                          |                                                                                                                                aCCCAACACCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGcc                                                                                                                               <  1:1210024/140‑1 (MQ=255)    cAACACCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATc                                                                                                                            <  2:146368/140‑1 (MQ=255)       cacCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGcc                                                                                                                               >  2:881251/1‑134 (MQ=255)       cacCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAAt                                                                                                                         >  1:1049397/1‑140 (MQ=255)       cacCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAAt                                                                                                                         >  1:840570/1‑140 (MQ=255)       cacCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAAt                                                                                                                         >  2:251227/1‑140 (MQ=255)             aTTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTAc                                                                                                                   >  1:1440140/1‑140 (MQ=255)                    tACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCgg                                                                                                            >  1:268076/1‑140 (MQ=255)                    tACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCgg                                                                                                            >  2:891878/1‑140 (MQ=255)                    tACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCgg                                                                                                            >  2:175813/1‑140 (MQ=255)                       ccTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGaaaa                                                                                                         <  1:553604/140‑1 (MQ=255)                       ccTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGaaa                                                                                                         >  1:1131320/1‑140 (MQ=255)                           aCAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAAcgcc                                                                                                     >  2:579424/1‑140 (MQ=255)                            cAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAAcgccg                                                                                                    >  2:51675/1‑140 (MQ=255)                               aCCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaa                                                                                                 <  2:341162/140‑1 (MQ=255)                                ccTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaac                                                                                                >  2:152534/1‑140 (MQ=255)                                ccTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaac                                                                                                >  2:570061/1‑140 (MQ=255)                                ccTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaac                                                                                                >  2:1049597/1‑140 (MQ=255)                                ccTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaac                                                                                                >  1:74820/1‑140 (MQ=255)                                 cTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaact                                                                                               >  2:797293/1‑140 (MQ=255)                                 cTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaact                                                                                               <  1:389972/140‑1 (MQ=255)                                   gAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaactca                                                                                             <  2:1440140/140‑1 (MQ=255)                                    aaaaTAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaactcaa                                                                                            <  2:1269807/140‑1 (MQ=255)                                     aaaTAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGcaactcaac                                                                                           >  2:929514/1‑140 (MQ=255)                                        tAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGt                                                                                        <  1:339478/140‑1 (MQ=255)                                        tAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGt                                                                                        <  1:175813/140‑1 (MQ=255)                                        tAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGt                                                                                        <  2:840570/140‑1 (MQ=255)                                           gTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTaaa                                                                                     >  2:477252/1‑140 (MQ=255)                                           gTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTaaa                                                                                     >  1:290247/1‑140 (MQ=255)                                              gAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCg                                                                                  >  1:1465395/1‑140 (MQ=255)                                                    tagtTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGcc                                                                            <  1:1049597/140‑1 (MQ=255)                                                      gtTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  2:1262429/140‑1 (MQ=255)                                                      gtTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  2:785354/140‑1 (MQ=255)                                                      gtTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  2:1465395/140‑1 (MQ=255)                                                      gtTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  2:290247/140‑1 (MQ=255)                                                      gtTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  1:51675/140‑1 (MQ=255)                                                        tAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCt                                                                        <  1:570061/140‑1 (MQ=255)                                                        tAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCt                                                                        <  1:152534/140‑1 (MQ=255)                                                         aaaaaTCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCtt                                                                       >  2:1004729/1‑140 (MQ=255)                                                              tCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCt                                                                        <  2:154909/134‑1 (MQ=255)                                                              tCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCt                                                                        >  1:154909/1‑134 (MQ=255)                                                               cAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGTCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  1:1409776/131‑1 (MQ=255)                                                               cAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGTCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          >  2:1409776/1‑131 (MQ=255)                                                                 aaTGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          <  2:15748/129‑1 (MQ=255)                                                                 aaTGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGt                                                                          >  1:15748/1‑129 (MQ=255)                                                                 aaTGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAag                                                               >  2:764364/1‑140 (MQ=255)                                                                      tttCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGc                                                          <  1:929514/140‑1 (MQ=255)                                                                      tttCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGc                                                          <  1:251227/140‑1 (MQ=255)                                                                       ttCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCg                                                         <  1:764364/140‑1 (MQ=255)                                                                         cccccTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCgag                                                       <  1:1004729/140‑1 (MQ=255)                                                                         cccccTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCgag                                                       <  2:1131320/140‑1 (MQ=255)                                                                            ccTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATg                                                    >  2:1072850/1‑140 (MQ=255)                                                                               ttGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGtt                                                                  <  1:632018/123‑1 (MQ=255)                                                                               ttGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGtt                                                                  >  2:632018/1‑123 (MQ=255)                                                                               ttGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCgag                                                       <  1:74958/134‑1 (MQ=255)                                                                               ttGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCgag                                                       >  2:74958/1‑134 (MQ=255)                                                                               ttGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGtat                                                 >  1:674855/1‑140 (MQ=255)                                                                                 gATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAg                                               <  1:579424/140‑1 (MQ=255)                                                                                      aTACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTc                                          <  1:891878/140‑1 (MQ=255)                                                                                      aTACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTc                                          <  2:74820/140‑1 (MQ=255)                                                                                       tACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCa                                         >  2:18823/1‑140 (MQ=255)                                                                                            aTTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTc                                          >  1:1371238/1‑134 (MQ=255)                                                                                            aTTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTc                                          <  2:1371238/134‑1 (MQ=255)                                                                                                         aaGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGacaaca                                >  1:3804/1‑131 (MQ=255)                                                                                                         aaGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGacaaca                                <  2:3804/131‑1 (MQ=255)                                                                                                          aGGGGGCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTa                      <  1:1072850/140‑1 (MQ=255)                                                                                                               gCCAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctc                 <  1:231253/140‑1 (MQ=255)                                                                                                                ccAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctct                >  1:405796/1‑140 (MQ=255)                                                                                                                ccAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctct                >  1:447654/1‑140 (MQ=255)                                                                                                                ccAAAAATAGCAGCGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGctct                >  1:1129257/1‑140 (MQ=255)                                                                                                                        agcagcGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGt        <  2:674855/140‑1 (MQ=255)                                                                                                                           agcGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGcc     <  1:688354/140‑1 (MQ=255)                                                                                                                            gcGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCa    <  1:797293/140‑1 (MQ=255)                                                                                                                            gcGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCa    <  2:1129257/140‑1 (MQ=255)                                                                                                                            gcGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCa    <  1:477252/140‑1 (MQ=255)                                                                                                                            gcGTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCa    <  2:1236736/140‑1 (MQ=255)                                                                                                                              gTAACGTATCGAGCCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATc  >  1:393216/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                ACCCAACACCTTATTTCATTACCCTGACAGACCTGAAAATAGGTGGAAAAGTAGTTAAAAATCAAATGATTTCCCCCTTTGATAAATACCAATTTTCTCTGCCAAAGGGGGCCAAAAATAGCAGCGTAACGTATCGAACCATCAATGACTACGGGGCGGAAACGCCGCAACTCAACTGTAAATCGTAAGCCGTCTTCAGTTAAGAGAGCGAGATGTATAGAACTCATCGACAACACAGCCTGTTAAGCTCTGGTGGAGTGCCATC  >  NC_000913/999004‑999268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑