Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,052,491	G→A	A291V (GCC→GTC)	yccM ←	putative 4Fe‑4S membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,052,491	0	G	A	100.0%	306.9 / NA	87	A291V (GCC→GTC)	yccM	putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/49);  total (38/49)

CCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCA  >  NC_000913/1052355‑1052622                                                                                                                                         |                                                                                                                                    ccATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTa                                                                                                                                  <  1:1143356/140‑1 (MQ=255)  cATACTCCCGCATACATTGCGTAACCAATCACCGCAAACAAAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTAc                                                                                                                                 <  1:1075797/140‑1 (MQ=255)   aTACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTAcc                                                                                                                                <  2:109553/140‑1 (MQ=255)        cccGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACa                                                                                                                           <  2:431411/140‑1 (MQ=255)        cccGCATACATTGCGTAACCAATCACCGCAAACATAATCCACAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACa                                                                                                                           <  2:1069002/140‑1 (MQ=255)         ccGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACAt                                                                                                                          >  2:751818/1‑140 (MQ=255)         ccGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACAt                                                                                                                          >  1:74085/1‑140 (MQ=255)                 aTTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTAc                                                                                                                  <  1:468213/140‑1 (MQ=255)                 aTTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTAc                                                                                                                  <  1:637081/140‑1 (MQ=255)                   tGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGc                                                                                                                >  1:873347/1‑140 (MQ=255)                   tGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGc                                                                                                                <  1:751818/140‑1 (MQ=255)                    gCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTGCGCa                                                                                                               <  2:741002/140‑1 (MQ=255)                     cGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACa                                                                                                                           <  2:472999/127‑1 (MQ=255)                     cGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACa                                                                                                                           >  1:472999/1‑127 (MQ=255)                     cGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCaa                                                                                                              >  2:1463766/1‑140 (MQ=255)                     cGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCaa                                                                                                              >  1:1185166/1‑140 (MQ=255)                         aCCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTAc                                                                                                                  <  1:1287615/132‑1 (MQ=255)                         aCCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCaa                                                                                                              >  2:1116811/1‑136 (MQ=255)                         aCCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCaa                                                                                                              <  1:1116811/136‑1 (MQ=255)                         aCCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTGTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTAc                                                                                                                  >  2:1287615/1‑132 (MQ=255)                          ccAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGa                                                                                                                        <  2:177512/125‑1 (MQ=255)                          ccAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGa                                                                                                                        >  1:177512/1‑125 (MQ=255)                          ccAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCAt                                                                                                         <  2:873347/140‑1 (MQ=255)                          ccAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCAt                                                                                                         <  1:1463766/140‑1 (MQ=255)                          ccAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCAt                                                                                                         >  2:1079209/1‑140 (MQ=255)                            aaTCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATAc                                                                                                       <  1:1180407/140‑1 (MQ=255)                             aTCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGATTCTACGCAAGTCATACa                                                                                                      >  2:15688/1‑140 (MQ=255)                              tCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAg                                                                                                     <  1:474320/140‑1 (MQ=255)                               cACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGc                                                                                                    >  2:1340371/1‑140 (MQ=255)                               cACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGc                                                                                                    >  2:1101095/1‑140 (MQ=255)                               cACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGc                                                                                                    <  1:475640/140‑1 (MQ=255)                                 ccGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCg                                                                                                  <  1:49329/140‑1 (MQ=255)                                  cGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCAt                                                                                                         <  2:1159931/132‑1 (MQ=255)                                  cGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCAt                                                                                                         >  1:1159931/1‑132 (MQ=255)                                   gCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGATTCTACGCAAGTCATACAGCCGGt                                                                                                <  1:15688/140‑1 (MQ=255)                                   gCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGt                                                                                                <  1:1101095/140‑1 (MQ=255)                                    cAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTa                                                                                               >  2:420230/1‑140 (MQ=255)                                    cAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTCTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTa                                                                                               <  1:1020056/140‑1 (MQ=255)                                      aaCATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACa                                                                                             <  1:1340371/140‑1 (MQ=255)                                        cATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACAtt                                                                                           >  2:119175/1‑140 (MQ=255)                                           aaTCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCa                                                                                        <  2:74085/140‑1 (MQ=255)                                           aaTCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCa                                                                                        <  1:581006/140‑1 (MQ=255)                                           aaTCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCa                                                                                        <  1:31189/140‑1 (MQ=255)                                            aTCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCAc                                                                                       >  2:613743/1‑140 (MQ=255)                                                ccAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGtt                                                                                   <  1:1300045/140‑1 (MQ=255)                                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGtt                                                                                   >  2:274189/1‑138 (MQ=255)                                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGtt                                                                                   <  1:274189/138‑1 (MQ=255)                                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                                                 >  2:879615/1‑140 (MQ=255)                                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                                                 <  1:1465587/140‑1 (MQ=255)                                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                                                 >  2:1465587/1‑140 (MQ=255)                                                  aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTCGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                                                 >  2:681695/1‑140 (MQ=255)                                                     aCCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTAc                                                                              <  1:879615/140‑1 (MQ=255)                                                          tAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTTAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaa                                                                         >  2:623275/1‑140 (MQ=255)                                                             cGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATAGAGCCGGTACATTCCACTGTTCGTACCTGaatta                                                                      <  1:433598/140‑1 (MQ=255)                                                                  ttAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTa                                                                 >  1:692966/1‑140 (MQ=255)                                                                   tAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTAt                                                                <  1:543940/140‑1 (MQ=255)                                                                     aCCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCg                                                              <  1:1161108/140‑1 (MQ=255)                                                                     aCCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGCTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCg                                                              <  1:483243/140‑1 (MQ=255)                                                                        agccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                           <  2:527555/140‑1 (MQ=255)                                                                        agccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                           <  1:1384192/140‑1 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTAt                                                                <  1:141457/133‑1 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTAt                                                                >  2:141457/1‑133 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                           <  2:809199/138‑1 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                                                           >  1:809199/1‑138 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCgg                                                         >  2:1265450/1‑140 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCgg                                                         >  2:234792/1‑140 (MQ=255)                                                                          ccagACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCgg                                                         >  2:1165193/1‑140 (MQ=255)                                                                                    cAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGAt                                               >  1:1030604/1‑140 (MQ=255)                                                                                    cAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGAt                                               >  1:674232/1‑140 (MQ=255)                                                                                            tttttATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAAtt                                       >  2:398637/1‑140 (MQ=255)                                                                                                 aTTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTg                                  <  2:674232/140‑1 (MQ=255)                                                                                                  tttGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgc                                 <  1:119175/140‑1 (MQ=255)                                                                                                       cGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCAtt                            >  1:1074923/1‑140 (MQ=255)                                                                                                       cGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCAtt                            >  2:734380/1‑140 (MQ=255)                                                                                                         cAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTg                          >  2:1053661/1‑140 (MQ=255)                                                                                                           ggTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGcc                        <  1:734380/140‑1 (MQ=255)                                                                                                           ggTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGcc                        <  2:1185166/140‑1 (MQ=255)                                                                                                            gTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCca                       <  1:756801/140‑1 (MQ=255)                                                                                                            gTTTTTGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCca                       <  1:398637/140‑1 (MQ=255)                                                                                                                ttGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGt                   <  2:692966/140‑1 (MQ=255)                                                                                                                 tGCAGTGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTc                  >  1:642294/1‑140 (MQ=255)                                                                                                                      tGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATAc             >  2:1219926/1‑140 (MQ=255)                                                                                                                      tGAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATAc             >  2:1465418/1‑140 (MQ=255)                                                                                                                       gAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGt                   <  1:1424010/133‑1 (MQ=255)                                                                                                                       gAAAAGGTCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGt                   >  2:1424010/1‑133 (MQ=255)                                                                                                                              tCAATGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCg     <  1:623275/140‑1 (MQ=255)                                                                                                                                 aTGTTGAGACTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCa  <  1:420230/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGCATTTGCCACAGTCGATACAACTTTCGGCA  >  NC_000913/1052355‑1052622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑