Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,873	C→T	T4I (ACA→ATA)	opgH →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,873	0	C	T	100.0%	246.5 / NA	70	T4I (ACA→ATA)	opgH	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/36);  total (34/36)

ACCAAAGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGACAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGG  >  NC_000913/1110736‑1111008                                                                                                                                          |                                                                                                                                        aCCAAAGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATaa                                                                                                                                       <  2:364059/140‑1 (MQ=255)   cAAAGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACt                                                                                                                                     <  2:536157/140‑1 (MQ=255)     aaGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGa                                                                                                                                   >  1:860206/1‑140 (MQ=255)     aaGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGa                                                                                                                                   <  1:1069394/140‑1 (MQ=255)      aGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAg                                                                                                                                  <  2:520441/140‑1 (MQ=255)        gCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATaa                                                                                                                                       >  1:1215139/1‑133 (MQ=255)        gCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATaa                                                                                                                                       <  2:1215139/133‑1 (MQ=255)        gCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTa                                                                                                                                <  2:860206/140‑1 (MQ=255)         cTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTAc                                                                                                                               >  2:292633/1‑140 (MQ=255)           ggCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACAt                                                                                                                             >  2:648793/1‑140 (MQ=255)            gCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACt                                                                                                                                     >  2:631465/1‑131 (MQ=255)            gCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACt                                                                                                                                     <  1:631465/131‑1 (MQ=255)            gCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACAtt                                                                                                                            <  2:1337729/140‑1 (MQ=255)            gCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACAtt                                                                                                                            <  2:96560/140‑1 (MQ=255)                 tGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGc                                                                                                                       >  1:387175/1‑140 (MQ=255)                  ggTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCa                                                                                                                      <  1:648793/140‑1 (MQ=255)                  ggTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCa                                                                                                                      >  1:831511/1‑140 (MQ=255)                      aTGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGc                                                                                                                  >  2:287781/1‑140 (MQ=255)                         cGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCa                                                                                                               >  1:383517/1‑140 (MQ=255)                           tgtgAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATc                                                                                                             >  1:1383549/1‑140 (MQ=255)                           tgtgAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATc                                                                                                             >  2:547115/1‑140 (MQ=255)                                 agtgaaagATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGc                                                                                                                       >  1:456258/1‑124 (MQ=255)                                 agtgaaagATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGc                                                                                                                       <  2:456258/124‑1 (MQ=255)                                   tgaaagATGCCAAGAAAACCTCTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAg                                                                                                     >  1:487668/1‑140 (MQ=255)                                     aaagATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCg                                                                                                   >  1:1454188/1‑140 (MQ=255)                                          tGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGaaa                                                                                              >  1:315340/1‑140 (MQ=255)                                              aaGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCgg                                                                                          <  2:1454188/140‑1 (MQ=255)                                                gAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCa                                                                                        <  2:208127/140‑1 (MQ=255)                                                gAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCa                                                                                        <  2:458561/140‑1 (MQ=255)                                                gAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCa                                                                                        <  1:1145395/140‑1 (MQ=255)                                                     cctcTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGcc                                                                                   <  2:487668/137‑1 (MQ=255)                                                     ccACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGcc                                                                                   <  2:387175/140‑1 (MQ=255)                                                       aCTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGa                                                                                 <  1:361874/140‑1 (MQ=255)                                                                cGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGata                                                                        >  1:940977/1‑140 (MQ=255)                                                                  tgctgcGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATc                                                                      >  1:218603/1‑140 (MQ=255)                                                                    ctgcGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCcg                                                                    <  2:218603/140‑1 (MQ=255)                                                                      gcgcTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCcgcg                                                                  <  1:292633/140‑1 (MQ=255)                                                                                 tGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAg                                                       <  2:571982/140‑1 (MQ=255)                                                                                 tGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAg                                                       >  1:1284031/1‑140 (MQ=255)                                                                                      aTCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCt                                                  <  2:1383549/140‑1 (MQ=255)                                                                                      aTCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCt                                                  <  1:540173/140‑1 (MQ=255)                                                                                      aTCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCt                                                  >  1:1240409/1‑140 (MQ=255)                                                                                             gTTGAGTGAAACCTGGAGCTACCAGTTATCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGcc                                           >  1:656793/1‑140 (MQ=255)                                                                                              ttGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCg                                          <  2:813859/140‑1 (MQ=255)                                                                                                  gTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAaca                                      <  2:1284031/140‑1 (MQ=255)                                                                                                     aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCg                                   >  1:741974/1‑140 (MQ=255)                                                                                                     aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCg                                   >  1:700456/1‑140 (MQ=255)                                                                                                     aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCg                                   >  2:205817/1‑140 (MQ=255)                                                                                                     aaaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCg                                   >  2:793466/1‑140 (MQ=255)                                                                                                      aaCCTGGAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGc                                  >  1:195110/1‑140 (MQ=255)                                                                                                            gAGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTgg                            >  1:824960/1‑140 (MQ=255)                                                                                                             aGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTggg                           <  2:1240409/140‑1 (MQ=255)                                                                                                             aGCTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTggg                           <  1:88739/140‑1 (MQ=255)                                                                                                               cTACCAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGgcg                         <  1:793466/140‑1 (MQ=255)                                                                                                                  ccAGTTACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCgg                      >  2:309441/1‑140 (MQ=255)                                                                                                                       tACCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGggagga                 <  2:741974/140‑1 (MQ=255)                                                                                                                        aCCTGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGgat                >  2:925346/1‑140 (MQ=255)                                                                                                                           tGCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGgatgat             >  1:1168336/1‑140 (MQ=255)                                                                                                                            gCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTggg                           <  1:262886/125‑1 (MQ=255)                                                                                                                            gCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTggg                           >  2:262886/1‑125 (MQ=255)                                                                                                                            gCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGAtt            >  2:764077/1‑140 (MQ=255)                                                                                                                            gCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGAtt            >  2:1173713/1‑140 (MQ=255)                                                                                                                            gCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGAtt            >  2:232004/1‑140 (MQ=255)                                                                                                                            gCCAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGAtt            >  1:291221/1‑140 (MQ=255)                                                                                                                             ccAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGgcg                         <  1:986972/126‑1 (MQ=255)                                                                                                                             ccAATGAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGgcg                         >  2:986972/1‑126 (MQ=255)                                                                                                                                  gAATAAGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGc      <  2:291221/140‑1 (MQ=255)                                                                                                                                      aaGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAgg  <  2:195110/140‑1 (MQ=255)                                                                                                                                      aaGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAgg  <  2:1168336/140‑1 (MQ=255)                                                                                                                                      aaGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAgg  <  1:547115/140‑1 (MQ=255)                                                                                                                                      aaGATAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAgg  <  1:287781/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ACCAAAGGCTGGCGTCTGGTGATGCGTGTGAAAGTGAAAGATGCCAAGAAAACCACTGAAATGCGTGCTGCGCTGGTGAATGCCGATCAGACGTTGAGTGAAACCTGGAGCTACCAGTTACCTGCCAATGAATAAGACAACTGAGTACATTGACGCAATGCCCATCGCCGCAAGCGAGAAAGCGGCATTGCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCCGCAAGG  >  NC_000913/1110736‑1111008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑