Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,131,872	A→G	T7A (ACC→GCC)	flgD →	flagellar biosynthesis, initiation of hook assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,131,872	0	A	G	100.0%	279.7 / NA	80	T7A (ACC→GCC)	flgD	flagellar biosynthesis, initiation of hook assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/36);  total (44/36)

AGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCACCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGACCAATCCA  >  NC_000913/1131737‑1132006                                                                                                                                        |                                                                                                                                       agagaTGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAaccgccac                                                                                                                                    <  1:675468/140‑1 (MQ=255)   agaTGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCg                                                                                                                                  <  1:479535/140‑1 (MQ=255)     aTGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGAt                                                                                                                                >  1:900087/1‑140 (MQ=255)       ggTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATcc                                                                                                                              >  1:788590/1‑140 (MQ=255)       ggTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATcc                                                                                                                              >  2:885226/1‑140 (MQ=255)             cacCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACaaa                                                                                                                        >  2:791355/1‑140 (MQ=255)               ccATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATa                                                                                                                      >  1:1466349/1‑140 (MQ=255)                  tGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCg                                                                                                                   >  2:647695/1‑140 (MQ=255)                  tGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCg                                                                                                                   >  2:786076/1‑140 (MQ=255)                  tGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCg                                                                                                                   >  2:183037/1‑140 (MQ=255)                  tGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCg                                                                                                                   >  1:177816/1‑140 (MQ=255)                    tCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGc                                                                                                                 <  1:1313711/140‑1 (MQ=255)                    tCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGc                                                                                                                 <  1:885226/140‑1 (MQ=255)                     cGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCg                                                                                                                <  2:900087/140‑1 (MQ=255)                      ggCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGt                                                                                                               <  1:176734/140‑1 (MQ=255)                       gCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTc                                                                                                              <  2:1466349/140‑1 (MQ=255)                          tCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGt                                                                                                           <  2:177816/140‑1 (MQ=255)                          tCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAACTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCAGCGTCAGt                                                                                                           >  2:983546/1‑140 (MQ=255)                               cAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTaccac                                                                                                      <  2:1472677/140‑1 (MQ=255)                                            aaTGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCg                                                                                         >  2:754497/1‑140 (MQ=255)                                              tGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCt                                                                                       >  2:1454108/1‑140 (MQ=255)                                                   aaGTGCTCAACACGGTGAAGAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACgg                                                                                  >  1:864345/1‑140 (MQ=255)                                                   aaGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGc                                                                                        <  1:584758/134‑1 (MQ=255)                                                   aaGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGc                                                                                        >  2:584758/1‑134 (MQ=255)                                                        cTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTGTGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGgcagc                                                                             >  2:92533/1‑140 (MQ=255)                                                        cTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCg                                                                                         <  2:870741/128‑1 (MQ=255)                                                        cTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCg                                                                                         >  1:870741/1‑128 (MQ=255)                                                        cTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGgcagc                                                                             <  1:786076/140‑1 (MQ=255)                                                        cTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGgcagc                                                                             >  2:1007135/1‑140 (MQ=255)                                                          cAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCaa                                                                           >  2:304383/1‑140 (MQ=255)                                                             cacGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAAcgc                                                                        <  1:244279/140‑1 (MQ=255)                                                             cacGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATCGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCaa                                                                           <  2:75626/137‑1 (MQ=255)                                                             cacGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATCGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCaa                                                                           >  1:75626/1‑137 (MQ=255)                                                               cGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAAcgccg                                                                      <  1:791355/140‑1 (MQ=255)                                                               cGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAAcgccg                                                                      <  2:246044/140‑1 (MQ=255)                                                                  tGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAg                                                                   >  1:830331/1‑140 (MQ=255)                                                                  tGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAg                                                                   >  2:372669/1‑140 (MQ=255)                                                                       aGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTa                                                              <  1:754497/140‑1 (MQ=255)                                                                          atgatgCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAAcgc                                                                        >  1:294353/1‑127 (MQ=255)                                                                          atgatgCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAAcgc                                                                        <  2:294353/127‑1 (MQ=255)                                                                            gatgCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAg                                                         >  1:90645/1‑140 (MQ=255)                                                                            gatgCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAg                                                         >  1:539937/1‑140 (MQ=255)                                                                              tgCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCa                                                       >  1:955256/1‑140 (MQ=255)                                                                                  gAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGttt                                                   <  1:1454108/140‑1 (MQ=255)                                                                                   aaaaCCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGtttt                                                  >  1:215124/1‑140 (MQ=255)                                                                                                  ggTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCtggtg                                   >  2:365132/1‑140 (MQ=255)                                                                                                        tAAAGGAGAAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAg                             <  2:830331/140‑1 (MQ=255)                                                                                                              agaAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaa                       >  2:1085678/1‑140 (MQ=255)                                                                                                              agaAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaa                       >  2:1258159/1‑140 (MQ=255)                                                                                                              agaAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaa                       >  1:1214753/1‑140 (MQ=255)                                                                                                              agaAAGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTAACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaa                       >  1:669436/1‑140 (MQ=255)                                                                                                              agaAAGCTATGTCCATTGCGGCAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaa                       >  2:466846/1‑140 (MQ=255)                                                                                                                aaaGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaaaa                     >  1:1367321/1‑140 (MQ=255)                                                                                                                aaaGCTATATCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACATAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaaaaa                     >  1:1301206/1‑140 (MQ=255)                                                                                                                 aaGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAc                    <  2:1214753/140‑1 (MQ=255)                                                                                                                 aaGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAc                    <  1:983546/140‑1 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGCTTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   >  2:1182298/1‑140 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaa                        <  1:613547/135‑1 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGaa                        >  2:613547/1‑135 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   >  2:966244/1‑140 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   <  2:955256/140‑1 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   <  2:90645/140‑1 (MQ=255)                                                                                                                  aGCTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   <  1:304383/140‑1 (MQ=255)                                                                                                                    cTATGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAg                 >  2:797910/1‑140 (MQ=255)                                                                                                                       tGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGAc              <  2:215124/140‑1 (MQ=255)                                                                                                                       tGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGAc              <  2:1367321/140‑1 (MQ=255)                                                                                                                       tGTCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGAc              <  1:92533/140‑1 (MQ=255)                                                                                                                         tCCATTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGAccc            >  2:559958/1‑140 (MQ=255)                                                                                                                            aTTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAc         <  2:1203424/140‑1 (MQ=255)                                                                                                                            aTTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAc         <  2:539937/140‑1 (MQ=255)                                                                                                                            aTTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAc         <  2:241890/140‑1 (MQ=255)                                                                                                                            aTTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAc         >  2:1337476/1‑140 (MQ=255)                                                                                                                            aTTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAc         >  1:1381020/1‑140 (MQ=255)                                                                                                                            aTTGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTAACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAc         <  2:669436/140‑1 (MQ=255)                                                                                                                              tGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   <  2:202864/128‑1 (MQ=255)                                                                                                                              tGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAAcc                   >  1:202864/1‑128 (MQ=255)                                                                                                                              tGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAcca       >  2:530045/1‑140 (MQ=255)                                                                                                                              tGCGGTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAcca       >  1:978979/1‑140 (MQ=255)                                                                                                                                 ggTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAccaatc    <  2:379207/140‑1 (MQ=255)                                                                                                                                 ggTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAccaatc    <  1:966244/140‑1 (MQ=255)                                                                                                                                 ggTAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAccaatc    <  1:183037/140‑1 (MQ=255)                                                                                                                                   tAACCGCCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGAccaatcca  >  1:1273596/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       AGAGATGGTTAACACCATGTCGGCGTCACGCAGCTATCAGGCCAATGTTGAAGTGCTCAACACGGTGAAAAGCATGATGCTGAAAACCCTTACGCTCGGTCAATAAAGGAGAAAGCTATGTCCATTGCGGTAACCACCACCGATCCGACAAATACCGGCGTCAGTACCACCAGCAGTAGTTCGCTCACGGGCAGCAACGCCGCAGATTTACAAAGCAGTTTTCTGACTTTGCTGGTGGCGCAGCTGAAAAACCAGGACCCGACCAATCCA  >  NC_000913/1131737‑1132006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑