Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,164,550	T→C	G152G (GGT→GGC)	nagZ →	beta‑N‑acetylhexosaminidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,164,550	0	T	C	100.0%	232.6 / NA	67	G152G (GGT→GGC)	nagZ	beta‑N‑acetylhexosaminidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/32);  total (35/32)

GATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCA  >  NC_000913/1164418‑1164686                                                                                                                                     |                                                                                                                                         gATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGcatgcatg                                                                                                                                   <  1:1327811/140‑1 (MQ=255)    cGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAg                                                                                                                                <  2:1041729/140‑1 (MQ=255)     gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGc                                                                                                                               >  1:498551/1‑140 (MQ=255)               ttGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGaaa                                                                                                                     >  1:522399/1‑140 (MQ=255)               ttGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGaa                                                                                                                      <  1:1317211/139‑1 (MQ=255)               ttGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTACTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGaa                                                                                                                      >  2:1317211/1‑139 (MQ=255)                    atCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGAc                                                                                                                >  1:113995/1‑140 (MQ=255)                    atCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGAc                                                                                                                <  2:503548/140‑1 (MQ=255)                     tCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGAcc                                                                                                               >  1:1176690/1‑140 (MQ=255)                     tCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGAcc                                                                                                               >  1:491425/1‑140 (MQ=255)                     tCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGAcc                                                                                                               >  2:871936/1‑140 (MQ=255)                       aGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCgg                                                                                                             >  2:1167890/1‑140 (MQ=255)                          tttGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGaa                                                                                                          >  2:873937/1‑140 (MQ=255)                           ttGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGaaa                                                                                                         <  2:797647/140‑1 (MQ=255)                           ttGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGaaa                                                                                                         <  2:797793/140‑1 (MQ=255)                            tGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAac                                                                                                        >  2:671856/1‑140 (MQ=255)                            tGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAac                                                                                                        >  2:852010/1‑140 (MQ=255)                            tGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAac                                                                                                        >  2:936178/1‑140 (MQ=255)                             gcgcCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                       <  2:1176690/140‑1 (MQ=255)                             gcgcCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                       <  2:818670/140‑1 (MQ=255)                                       tGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGa                                                                                             <  1:1167890/140‑1 (MQ=255)                                         gATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGaca                                                                                           <  1:1134924/140‑1 (MQ=255)                                         gATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGaca                                                                                           <  2:484992/140‑1 (MQ=255)                                         gATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGaca                                                                                           <  1:671856/140‑1 (MQ=255)                                         gATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATACACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGaca                                                                                           <  1:1145255/140‑1 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                       >  2:1074908/1‑123 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                       <  1:1074908/123‑1 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                       <  1:806426/123‑1 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                       >  2:806426/1‑123 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGaca                                                                                           >  2:507581/1‑135 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGaca                                                                                           <  1:507581/135‑1 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTg                                                                                      >  1:945999/1‑140 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTg                                                                                      >  1:497566/1‑140 (MQ=255)                                              cGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTg                                                                                      >  1:478479/1‑140 (MQ=255)                                                ggCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                    >  1:246638/1‑140 (MQ=255)                                                ggCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTACCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                    >  2:1299203/1‑140 (MQ=255)                                                   atatCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTa                                                                                 >  2:314948/1‑140 (MQ=255)                                                         gcgcGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCa                                                                           <  2:478479/140‑1 (MQ=255)                                                               cgATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTca                                                                     <  1:852010/140‑1 (MQ=255)                                                                 aTTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcaca                                                                   <  2:113995/140‑1 (MQ=255)                                                                 aTTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcaca                                                                   >  2:784882/1‑140 (MQ=255)                                                                 aTTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcaca                                                                   <  1:890468/140‑1 (MQ=255)                                                                 aTTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcaca                                                                   <  1:873937/140‑1 (MQ=255)                                                                         gCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGaaa                                                           >  1:305192/1‑140 (MQ=255)                                                                                tATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTg                                                    <  2:945999/140‑1 (MQ=255)                                                                                 aTCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGc                                                   <  2:338971/140‑1 (MQ=255)                                                                                 aTCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGc                                                   >  1:1271140/1‑140 (MQ=255)                                                                                    aTGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcacaca                                                                 <  2:391597/123‑1 (MQ=255)                                                                                    aTGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcacaca                                                                 >  1:391597/1‑123 (MQ=255)                                                                                    aTGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGAt                                                >  1:391805/1‑140 (MQ=255)                                                                                      gCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATcc                                              <  1:307219/140‑1 (MQ=255)                                                                                      gCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATcc                                              >  1:504537/1‑140 (MQ=255)                                                                                       ccGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCa                                             >  2:1429903/1‑140 (MQ=255)                                                                                       ccGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCa                                             >  2:1412359/1‑140 (MQ=255)                                                                                       ccGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCa                                             >  2:610621/1‑140 (MQ=255)                                                                                       ccGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCa                                             >  2:673291/1‑140 (MQ=255)                                                                                           tCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTc                                         >  2:349891/1‑140 (MQ=255)                                                                                              acaAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcac                                      <  1:936178/140‑1 (MQ=255)                                                                                                       ccTGGCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGgaga                             >  2:605207/1‑140 (MQ=255)                                                                                                          ggCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTc                          >  1:1236322/1‑140 (MQ=255)                                                                                                           gCAATTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTcg                         <  1:673291/140‑1 (MQ=255)                                                                                                              aTTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCgcgc                      >  2:286471/1‑140 (MQ=255)                                                                                                              aTTGCCAGCCGGTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCgcgc                      <  1:349891/140‑1 (MQ=255)                                                                                                                         gTTTATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGt           >  1:1097711/1‑140 (MQ=255)                                                                                                                            tATTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCgg        <  2:497566/140‑1 (MQ=255)                                                                                                                             aTTGATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGt       <  1:1299203/140‑1 (MQ=255)                                                                                                                                gATGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCtt    <  2:246638/140‑1 (MQ=255)                                                                                                                                  tGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCa  <  2:214892/140‑1 (MQ=255)                                                                                                                                  tGGCATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCa  <  2:391805/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCA  >  NC_000913/1164418‑1164686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑