Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,172,514	A→G	V484A (GTC→GCC)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,172,514	0	A	G	100.0%	246.2 / NA	71	V484A (GTC→GCC)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/37);  total (34/37)

AGATGCAGTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACG  >  NC_000913/1172382‑1172650                                                                                                                                     |                                                                                                                                         aGATGCAGTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCgg                                                                                                                                   <  1:1306680/140‑1 (MQ=255)   aTGCAGTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCt                                                                                                                                 >  2:357584/1‑140 (MQ=255)   aTGCAGTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCt                                                                                                                                 <  2:661038/140‑1 (MQ=255)      cAGTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGAc                                                                                                                              <  1:1237682/140‑1 (MQ=255)        gTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCa                                                                                                                            >  2:1200010/1‑140 (MQ=255)        gTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCa                                                                                                                            >  1:397243/1‑140 (MQ=255)         tGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGAc                                                                                                                              <  1:1196189/137‑1 (MQ=255)         tGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGAc                                                                                                                              >  2:1196189/1‑137 (MQ=255)           acgacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGa                                                                                                                               >  1:824785/1‑134 (MQ=255)           acgacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGa                                                                                                                               <  2:824785/134‑1 (MQ=255)           acgacACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAata                                                                                                                         >  2:831521/1‑140 (MQ=255)              acacCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGAc                                                                                                                              <  1:629425/132‑1 (MQ=255)              acacCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGAc                                                                                                                              >  2:629425/1‑132 (MQ=255)              acacCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGc                                                                                                                      <  2:337210/140‑1 (MQ=255)               cacCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCa                                                                                                                     <  1:1200010/140‑1 (MQ=255)                acCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAg                                                                                                                    >  2:590259/1‑140 (MQ=255)                  cGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGtt                                                                                                                  >  2:149162/1‑140 (MQ=255)                      aCATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGc                                                                                                              <  2:397243/140‑1 (MQ=255)                       cATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCa                                                                                                             <  1:669073/140‑1 (MQ=255)                        aTACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATCCTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCaa                                                                                                            <  2:35246/140‑1 (MQ=255)                        aTACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCaa                                                                                                            <  1:264858/140‑1 (MQ=255)                        aTACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCaa                                                                                                            <  1:590259/140‑1 (MQ=255)                             gTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTa                                                                                                       <  1:1143549/140‑1 (MQ=255)                              tttGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTAc                                                                                                      >  2:313638/1‑140 (MQ=255)                               ttGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATg                                                                                                                       <  1:213148/122‑1 (MQ=255)                               ttGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATg                                                                                                                       >  2:213148/1‑122 (MQ=255)                               ttGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACg                                                                                                     <  2:1115307/140‑1 (MQ=255)                               ttGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACg                                                                                                     >  1:1309534/1‑140 (MQ=255)                                 ggCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGa                                                                                                   <  1:149162/140‑1 (MQ=255)                                  gCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGAt                                                                                                  <  2:1309534/140‑1 (MQ=255)                                   cgtcgtTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGc                                                                                                              >  1:874458/1‑127 (MQ=255)                                   cgtcgtTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGc                                                                                                              <  2:874458/127‑1 (MQ=255)                                   cgtcgtTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATc                                                                                                 >  1:897757/1‑140 (MQ=255)                                      cgtTGGCATAGGTGAGCATCAGATACTCGCCAATAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACgg                                                                                                    >  2:1295340/1‑134 (MQ=255)                                      cgtTGGCATAGGTGAGCATCAGATACTCGCCAATAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACgg                                                                                                    <  1:1295340/134‑1 (MQ=255)                                        ttGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTTACCAATATGCAGTTCCGCAAGGTTACGGATCAgtgt                                                                                            >  2:593922/1‑140 (MQ=255)                                        ttGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAgtgt                                                                                            >  1:496419/1‑140 (MQ=255)                                           gCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATc                                                                                         <  1:776546/140‑1 (MQ=255)                                                 gTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGtt                                                                                   >  2:1084996/1‑140 (MQ=255)                                                     gCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATg                                                                               <  2:259486/140‑1 (MQ=255)                                                            aTACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTgcggc                                                                        <  1:415982/140‑1 (MQ=255)                                                                 cGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGaa                                                                   >  1:934076/1‑140 (MQ=255)                                                                  gCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTgc                                                                           >  2:1296101/1‑131 (MQ=255)                                                                  gCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTgc                                                                           <  1:1296101/131‑1 (MQ=255)                                                                   ccAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATc                                                                 >  1:1039775/1‑140 (MQ=255)                                                                   ccAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATc                                                                 >  2:593004/1‑140 (MQ=255)                                                                     aGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCt                                                               >  1:1388574/1‑140 (MQ=255)                                                                                 cGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACggg                                                   >  2:640687/1‑140 (MQ=255)                                                                                  gCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGc                                                  <  2:1388574/140‑1 (MQ=255)                                                                                  gCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGc                                                  <  1:313638/140‑1 (MQ=255)                                                                                  gCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGc                                                  <  2:496419/140‑1 (MQ=255)                                                                                          cGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGtt                                          >  1:1010443/1‑140 (MQ=255)                                                                                            tGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGt                                           >  2:1237415/1‑137 (MQ=255)                                                                                            tGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGt                                           <  1:1237415/137‑1 (MQ=255)                                                                                              gTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCAcc                                      >  2:1095115/1‑140 (MQ=255)                                                                                              gTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCAcc                                      >  2:542987/1‑140 (MQ=255)                                                                                              gTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCAcc                                      >  2:502071/1‑140 (MQ=255)                                                                                               tCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCg                                     <  2:1039775/140‑1 (MQ=255)                                                                                                 aTTCCGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAg                                   <  1:1095115/140‑1 (MQ=255)                                                                                                    ccGGCATAACGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAg                                >  1:264814/1‑140 (MQ=255)                                                                                                            aCGACCGACGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCttt                        >  1:567489/1‑140 (MQ=255)                                                                                                             cgaccgacGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTc                       >  1:492114/1‑140 (MQ=255)                                                                                                              gaccgacGCCGTGCTCCAGATGGGCCACCGGCTTACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCg                      <  1:593922/140‑1 (MQ=255)                                                                                                              gaccgacGCCGTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCg                      <  1:502071/140‑1 (MQ=255)                                                                                                                        gTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAgcg            <  2:264814/140‑1 (MQ=255)                                                                                                                        gTGCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAgcg            >  2:768734/1‑140 (MQ=255)                                                                                                                          gCTCCAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGcc          <  1:542987/140‑1 (MQ=255)                                                                                                                              cAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGcc          <  2:661138/136‑1 (MQ=255)                                                                                                                              cAGATGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGcc          >  1:661138/1‑136 (MQ=255)                                                                                                                                  tGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACg  <  2:492114/140‑1 (MQ=255)                                                                                                                                  tGGGCCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACg  <  1:640687/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         AGATGCAGTGACGACACCGGAACATACAGTTTGGCGTCGTTGGCATAGGTGAGCATCAGATACTCGCCAGTAATGCCACCCGCTTCCAGCGTGGTCATTCCGGCATAACGACCGACGCCGTGCTCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACG  >  NC_000913/1172382‑1172650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑