Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,266,530	T→C	V146A (GTC→GCC)	prmC →	protein‑(glutamine‑N(5)) methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,266,530	0	T	C	100.0%	319.3 / NA	91	V146A (GTC→GCC)	prmC	protein‑(glutamine‑N(5)) methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/44);  total (47/44)

CAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAGGA  >  NC_000913/1266394‑1266665                                                                                                                                         |                                                                                                                                        cAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGcctc                                                                                                                                      >  2:555052/1‑140 (MQ=255) cAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGcctc                                                                                                                                      >  1:991138/1‑140 (MQ=255) cAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGcctc                                                                                                                                      >  1:51465/1‑140 (MQ=255)   ggCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTccc                                                                                                                                    >  2:1320586/1‑140 (MQ=255)      aCTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTgg                                                                                                                                 >  1:882960/1‑140 (MQ=255)         ggCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGcac                                                                                                                              <  2:760362/140‑1 (MQ=255)         ggCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGcac                                                                                                                              <  2:504061/140‑1 (MQ=255)          gcgcGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGcaca                                                                                                                             <  1:48166/140‑1 (MQ=255)              ggTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGt                                                                                                                         >  2:680142/1‑140 (MQ=255)               gTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTa                                                                                                                        <  1:680142/140‑1 (MQ=255)                ttGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTaa                                                                                                                       <  1:272696/140‑1 (MQ=255)                    cTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGcc                                                                                                                   >  2:1239941/1‑140 (MQ=255)                    cTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGcc                                                                                                                   <  1:613655/140‑1 (MQ=255)                    cTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGcc                                                                                                                   <  1:1320586/140‑1 (MQ=255)                            ccTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCt                                                                                                           >  2:872654/1‑140 (MQ=255)                            ccTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCt                                                                                                           >  1:457760/1‑140 (MQ=255)                             cTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTg                                                                                                          >  1:201542/1‑140 (MQ=255)                              ttGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGc                                                                                                                    >  1:1440613/1‑129 (MQ=255)                              ttGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGc                                                                                                                    <  2:1440613/129‑1 (MQ=255)                              ttGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTgg                                                                                                         >  1:631196/1‑140 (MQ=255)                                 ccGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGa                                                                                                      >  2:652073/1‑140 (MQ=255)                                 ccGTATTCTCGATCTCGAGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGa                                                                                                      >  2:1385956/1‑140 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaa                                                                                                 <  1:872654/140‑1 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaa                                                                                                 >  1:984094/1‑140 (MQ=255)                                      ttCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaa                                                                                                 >  1:596728/1‑140 (MQ=255)                                        ctcGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaaaa                                                                                               <  2:681640/140‑1 (MQ=255)                                           gATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGTGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAatat                                                                                            <  2:409515/140‑1 (MQ=255)                                           gATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAatat                                                                                            <  2:51465/140‑1 (MQ=255)                                              ctcGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCca                                                                                         >  2:1027549/1‑140 (MQ=255)                                                cGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaaaa                                                                                               <  2:735846/132‑1 (MQ=255)                                                cGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCaaaaa                                                                                               >  1:735846/1‑132 (MQ=255)                                                cGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCcaca                                                                                       >  2:666068/1‑140 (MQ=255)                                                 gggACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAt                                                                                      <  1:1185411/140‑1 (MQ=255)                                                 gggACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACAt                                                                                      >  2:159416/1‑140 (MQ=255)                                                       ggTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCa                                                                                >  2:1119772/1‑140 (MQ=255)                                                        gTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCaa                                                                               >  1:440252/1‑140 (MQ=255)                                                         tACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCaaa                                                                              <  2:882960/140‑1 (MQ=255)                                                         tACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCaaa                                                                              >  1:43199/1‑140 (MQ=255)                                                             ggggCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGa                                                                          <  1:1239941/140‑1 (MQ=255)                                                               ggCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTACGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACt                                                                        >  1:9386/1‑140 (MQ=255)                                                                  gATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGc                                                                            <  2:1312551/133‑1 (MQ=255)                                                                  gATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGc                                                                            >  1:1312551/1‑133 (MQ=255)                                                                   aTTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGtt                                                                    >  2:660075/1‑140 (MQ=255)                                                                   aTTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCCGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGtt                                                                    >  1:1165542/1‑140 (MQ=255)                                                                     tgcgcttgcgctGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTa                                                                  <  2:631196/140‑1 (MQ=255)                                                                        gcttgcgctGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGa                                                                          >  1:119436/1‑129 (MQ=255)                                                                        gcttgcgctGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGa                                                                          <  2:119436/129‑1 (MQ=255)                                                                          ttgcgctGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAgcgcg                                                             <  2:596728/140‑1 (MQ=255)                                                                              gctGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAg                                                         <  1:666068/140‑1 (MQ=255)                                                                                 ggCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCg                                                      >  2:1332135/1‑140 (MQ=255)                                                                                       cgagcgCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGgcagc                                                <  2:457760/140‑1 (MQ=255)                                                                                         agcgCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATCCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGcagcag                                              <  2:249835/140‑1 (MQ=255)                                                                                         agcgCCCGGACTGCGAAATTATCGCTGTAGATCGTACGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGcagcag                                              <  2:9386/140‑1 (MQ=255)                                                                                            gcCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGgca                                                  <  2:1301404/133‑1 (MQ=255)                                                                                            gcCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGgca                                                  >  1:1301404/1‑133 (MQ=255)                                                                                            gcCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGttt                                           >  1:596071/1‑140 (MQ=255)                                                                                            gcCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGttt                                           >  1:1291213/1‑140 (MQ=255)                                                                                                      cGAAATTATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTTGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTg                                 >  2:776726/1‑140 (MQ=255)                                                                                                            tATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCa                           <  1:652073/140‑1 (MQ=255)                                                                                                            tATCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCa                           <  2:43199/140‑1 (MQ=255)                                                                                                              tCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAAt                         <  1:1027549/140‑1 (MQ=255)                                                                                                              tCGCTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAAt                         >  2:1441955/1‑140 (MQ=255)                                                                                                                 cTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                      <  2:991138/140‑1 (MQ=255)                                                                                                                 cTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                      <  1:1369382/140‑1 (MQ=255)                                                                                                                 cTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                      <  2:201542/140‑1 (MQ=255)                                                                                                                 cTGTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                      <  1:778221/140‑1 (MQ=255)                                                                                                                   gTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccgcc                    <  1:555052/140‑1 (MQ=255)                                                                                                                   gTAGATCGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccgcc                    <  1:1119772/140‑1 (MQ=255)                                                                                                                         cGTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTatat              >  2:1302020/1‑140 (MQ=255)                                                                                                                          gTATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATAtt             >  2:1446265/1‑140 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAGCGTAATGCCAAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAAt                         >  2:1235529/1‑127 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAGCGTAATGCCAAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAAt                         <  1:1235529/127‑1 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                      >  2:62402/1‑130 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccg                      <  1:62402/130‑1 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTg            >  2:1395331/1‑140 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTg            <  1:969979/140‑1 (MQ=255)                                                                                                                           tATGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTg            >  2:969979/1‑140 (MQ=255)                                                                                                                            aTGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGa           >  2:140074/1‑140 (MQ=255)                                                                                                                            aTGCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGa           <  1:1332135/140‑1 (MQ=255)                                                                                                                              gCCTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACg         >  1:1430727/1‑140 (MQ=255)                                                                                                                                cTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccgcc                    >  2:1434049/1‑127 (MQ=255)                                                                                                                                cTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATccgcc                    <  1:1434049/127‑1 (MQ=255)                                                                                                                                cTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAg       >  1:1432259/1‑140 (MQ=255)                                                                                                                                cTGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAg       <  1:1446265/140‑1 (MQ=255)                                                                                                                                 tGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGAc          <  2:481819/136‑1 (MQ=255)                                                                                                                                 tGATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGAc          >  1:481819/1‑136 (MQ=255)                                                                                                                                  gATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCa     >  2:1293255/1‑140 (MQ=255)                                                                                                                                  gATGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCa     >  2:1185215/1‑140 (MQ=255)                                                                                                                                   aTGCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAg    >  2:610694/1‑140 (MQ=255)                                                                                                                                    tgctgcCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAgg   >  2:464178/1‑140 (MQ=255)                                                                                                                                     gCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGta                 <  2:109586/125‑1 (MQ=255)                                                                                                                                     gCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGta                 >  1:109586/1‑125 (MQ=255)                                                                                                                                     gCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAg       >  2:753488/1‑135 (MQ=255)                                                                                                                                     gCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAg       <  1:753488/135‑1 (MQ=255)                                                                                                                                     gCTGCCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAGGa  <  1:1395331/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGGCACTGGCGCGGTTGCCTGAACAACCTTGCCGTATTCTCGATCTCGGGACGGGTACCGGGGCGATTGCGCTTGCGCTGGCTAGCGAGCGCCCGGACTGCGAAATTATCGCTGTAGATCGTATGCCTGATGCTGTCTCCCTGGCACAACGTAATGCCCAGCATCTGGCGATCAAAAATATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGCAATCCGCCGTATATTGACGAGCAGGA  >  NC_000913/1266394‑1266665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑