Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,410	T→C	D70G (GAC→GGC)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,410	0	T	C	100.0%	330.8 / NA	96	D70G (GAC→GGC)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/47);  total (49/47)

GCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTCCAAT  >  NC_000913/1277273‑1277546                                                                                                                                          |                                                                                                                                         gCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCt                                                                                                                                        >  2:960236/1‑140 (MQ=255)  cATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCtt                                                                                                                                       <  2:230716/140‑1 (MQ=255)     cAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTtct                                                                                                                                    <  1:1410433/140‑1 (MQ=255)               aTCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATgg                                                                                                                          >  2:826104/1‑140 (MQ=255)                  aGTTCATTACGCCAGTAATTTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCAc                                                                                                                       >  2:1285060/1‑140 (MQ=255)                  aGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCAc                                                                                                                       >  2:223590/1‑140 (MQ=255)                  aGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCAc                                                                                                                       >  2:845077/1‑140 (MQ=255)                  aGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCAc                                                                                                                       >  2:1188084/1‑140 (MQ=255)                   gTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACt                                                                                                                      <  1:1006540/140‑1 (MQ=255)                     tCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTgc                                                                                                                    <  2:583362/140‑1 (MQ=255)                       aTTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTgccg                                                                                                                  >  1:31254/1‑140 (MQ=255)                         tACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATg                                                                                                                           <  2:223600/129‑1 (MQ=255)                         tACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATg                                                                                                                           >  1:223600/1‑129 (MQ=255)                         tACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTgccgcc                                                                                                                >  2:964726/1‑140 (MQ=255)                         tACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTgccgcc                                                                                                                >  2:1332809/1‑140 (MQ=255)                         tACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTgccgcc                                                                                                                >  2:428170/1‑140 (MQ=255)                         tACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTgccgcc                                                                                                                >  2:595931/1‑140 (MQ=255)                              cAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCTGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTaa                                                                                                                             >  2:105456/1‑122 (MQ=255)                              cAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCTGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTaa                                                                                                                             <  1:105456/122‑1 (MQ=255)                              cAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAg                                                                                                           >  1:242554/1‑140 (MQ=255)                                   aTCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGt                                                                                                      <  1:595931/140‑1 (MQ=255)                                   aTCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGt                                                                                                      <  1:1368728/140‑1 (MQ=255)                                           aaCCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGc                                                                                              >  2:709796/1‑140 (MQ=255)                                              ccTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACg                                                                                                        >  1:550280/1‑127 (MQ=255)                                              ccTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACg                                                                                                        <  2:550280/127‑1 (MQ=255)                                                tGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACttt                                                                                                <  1:339598/133‑1 (MQ=255)                                                tGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACttt                                                                                                >  2:339598/1‑133 (MQ=255)                                                tGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATgcg                                                                                         >  1:35241/1‑140 (MQ=255)                                                tGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATgcg                                                                                         >  2:352920/1‑140 (MQ=255)                                                 gTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATgcgc                                                                                        <  1:223590/140‑1 (MQ=255)                                                  tAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCa                                                                                       >  1:222478/1‑140 (MQ=255)                                                        cgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATc                                                                                 <  2:770916/140‑1 (MQ=255)                                                        cgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATc                                                                                 >  2:1171869/1‑140 (MQ=255)                                                        cgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATc                                                                                 >  2:241212/1‑140 (MQ=255)                                                        cgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATc                                                                                 >  1:655645/1‑140 (MQ=255)                                                        cgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATc                                                                                 >  2:3029/1‑140 (MQ=255)                                                        cgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATc                                                                                 <  1:241212/140‑1 (MQ=255)                                                             gTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCt                                                                            >  1:121741/1‑140 (MQ=255)                                                                  ccGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTttgtt                                                                       <  2:1479543/140‑1 (MQ=255)                                                                  ccGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTttgtt                                                                       <  1:1285060/140‑1 (MQ=255)                                                                        cGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGc                                                                 <  1:1031721/140‑1 (MQ=255)                                                                        cGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGc                                                                 >  2:606256/1‑140 (MQ=255)                                                                        cGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGc                                                                 >  2:46914/1‑140 (MQ=255)                                                                          ttCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCAt                                                               <  1:964726/140‑1 (MQ=255)                                                                               ctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcg                                                          <  2:242554/140‑1 (MQ=255)                                                                               ctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcg                                                          <  1:636684/140‑1 (MQ=255)                                                                               ctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcg                                                          <  2:31254/140‑1 (MQ=255)                                                                               ctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcg                                                          <  2:1084791/140‑1 (MQ=255)                                                                               ctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCCCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcg                                                          <  1:675807/140‑1 (MQ=255)                                                                                  ctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTg                                                       >  1:36126/1‑140 (MQ=255)                                                                                   tCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGc                                                      >  1:1026805/1‑140 (MQ=255)                                                                                       gTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTg                                                  >  2:1329140/1‑140 (MQ=255)                                                                                        tCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGa                                                 <  1:960236/140‑1 (MQ=255)                                                                                         cAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGaa                                                <  2:655645/140‑1 (MQ=255)                                                                                         cAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGaa                                                <  2:121741/140‑1 (MQ=255)                                                                                               ggCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCtt                                          >  1:411417/1‑140 (MQ=255)                                                                                                gcgcTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTg                                         <  1:1332809/140‑1 (MQ=255)                                                                                                 cgcTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTGACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGc                                        >  2:1022574/1‑140 (MQ=255)                                                                                                 cgcTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGc                                        >  2:1345736/1‑140 (MQ=255)                                                                                                 cgcTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGc                                        >  1:64367/1‑140 (MQ=255)                                                                                                   cTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAc                                      <  2:35241/140‑1 (MQ=255)                                                                                                      aaTGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccag                                   >  1:1462590/1‑140 (MQ=255)                                                                                                      aaTGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccag                                   <  1:352920/140‑1 (MQ=255)                                                                                                       aTGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccagc                                  <  2:1295512/140‑1 (MQ=255)                                                                                                       aTGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccagc                                  <  1:1345736/140‑1 (MQ=255)                                                                                                       aTGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccagc                                  <  2:48873/140‑1 (MQ=255)                                                                                                           cGTTTGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccagccagc                              >  1:934789/1‑140 (MQ=255)                                                                                                              ttGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccagccagccag                           >  2:424021/1‑140 (MQ=255)                                                                                                               tGTTCCATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGa                          <  2:222478/140‑1 (MQ=255)                                                                                                                    cATCTCTTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCg                     <  2:1026805/140‑1 (MQ=255)                                                                                                                       ctctTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCa                  >  1:1348715/1‑140 (MQ=255)                                                                                                                         ttttAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATc                <  2:694095/139‑1 (MQ=255)                                                                                                                         ctTTAATTAAGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATc                >  1:462218/1‑140 (MQ=255)                                                                                                                           ttaattaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGGTCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATccc              >  1:362300/1‑140 (MQ=255)                                                                                                                            taattaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCt             >  1:1009000/1‑140 (MQ=255)                                                                                                                            taattaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCt             <  2:775242/140‑1 (MQ=255)                                                                                                                            taattaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCt             >  1:1141276/1‑140 (MQ=255)                                                                                                                              attaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGGTCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGc           <  2:362300/140‑1 (MQ=255)                                                                                                                              attaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGc           <  1:46914/140‑1 (MQ=255)                                                                                                                              attaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGc           <  1:3029/140‑1 (MQ=255)                                                                                                                              attaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTCTGTTTATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGc           <  1:1188084/140‑1 (MQ=255)                                                                                                                               ttaaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGcc          <  1:826104/140‑1 (MQ=255)                                                                                                                                 aaGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAg        <  1:445312/140‑1 (MQ=255)                                                                                                                                  aGGGCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGt       >  1:869165/1‑140 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTGACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     <  1:1022574/140‑1 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCg                     >  1:1157798/1‑124 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCg                     <  2:1157798/124‑1 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     >  2:1254271/1‑140 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     >  2:1228097/1‑140 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     >  2:1140374/1‑140 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     <  1:709796/140‑1 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     <  1:606256/140‑1 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     >  1:49377/1‑140 (MQ=255)                                                                                                                                    ggCTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTcc     >  2:455636/1‑140 (MQ=255)                                                                                                                                      cTTGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTCCaa   <  1:424021/140‑1 (MQ=255)                                                                                                                                       ttGCCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTCCAAt  <  2:64367/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCCCTGCCAGTCCAAT  >  NC_000913/1277273‑1277546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑