Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,607	A→G	intergenic (‑127/+37)	thiB ← / ← sgrR	thiamine ABC transporter periplasmic binding protein/DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,607	0	A	G	100.0%	300.0 / NA	86	intergenic (‑127/+37)	thiB/sgrR	thiamine ABC transporter periplasmic binding protein/DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/38);  total (48/38)

TTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGC  >  NC_000913/75471‑75743                                                                                                                                         |                                                                                                                                         tttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCgg                                                                                                                                       >  2:1005132/1‑140 (MQ=255) tttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCgg                                                                                                                                       >  2:503590/1‑140 (MQ=255)   tttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAgcggcg                                                                                                                                     >  2:653273/1‑140 (MQ=255)    ttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGt                                                                                                                                    <  2:740206/140‑1 (MQ=255)        cacTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTtagt                                                                                                                                >  2:125126/1‑140 (MQ=255)        cacTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTtagt                                                                                                                                >  1:1294616/1‑140 (MQ=255)           tttGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTtagtgta                                                                                                                             >  2:389509/1‑140 (MQ=255)             tGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGTACCCCGTTGAGAGCGGCGTtagtgtagt                                                                                                                           >  2:547393/1‑140 (MQ=255)                  cTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGAttt                                                                                                                      >  2:1278427/1‑140 (MQ=255)                    cAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTg                                                                                                                    >  2:146635/1‑140 (MQ=255)                    cAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTg                                                                                                                    >  2:781963/1‑140 (MQ=255)                       aaaaGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTa                                                                                                                 <  2:198607/140‑1 (MQ=255)                             gTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAAcc                                                                                                           >  2:403878/1‑140 (MQ=255)                              tGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAAc                                                                                                            <  1:1447582/138‑1 (MQ=255)                              tGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAAc                                                                                                            >  2:1416499/1‑138 (MQ=255)                              tGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAAc                                                                                                            <  1:1416499/138‑1 (MQ=255)                              tGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAAc                                                                                                            >  2:1447582/1‑138 (MQ=255)                              tGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCa                                                                                                          >  1:255076/1‑140 (MQ=255)                                gCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAg                                                                                                         >  1:891084/1‑139 (MQ=255)                                gCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAg                                                                                                         <  2:891084/139‑1 (MQ=255)                                   aaGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAAt                                                                                                     <  1:125126/140‑1 (MQ=255)                                              aaGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGTACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCgg                                                                                          <  1:547393/140‑1 (MQ=255)                                                ggAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTg                                                                                        >  2:71899/1‑140 (MQ=255)                                                 gAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTgg                                                                                       >  2:351804/1‑140 (MQ=255)                                                 gAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTgg                                                                                       >  2:270222/1‑140 (MQ=255)                                                 gAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTgg                                                                                       >  2:476710/1‑140 (MQ=255)                                                 gAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTgg                                                                                       >  2:1241339/1‑140 (MQ=255)                                                  aGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGgc                                                                                      <  1:1005132/140‑1 (MQ=255)                                                   gCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGgcg                                                                                     <  1:1241339/140‑1 (MQ=255)                                                   gCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGgcg                                                                                     >  1:143387/1‑140 (MQ=255)                                                   gCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGATTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGgcg                                                                                     <  2:1023544/140‑1 (MQ=255)                                                      tCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCg                                                                                           >  1:944775/1‑131 (MQ=255)                                                      tCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCg                                                                                           <  2:944775/131‑1 (MQ=255)                                                      tCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGt                                                                                         >  2:472968/1‑133 (MQ=255)                                                      tCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGt                                                                                         <  1:472968/133‑1 (MQ=255)                                                       cAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCaaa                                                                                 >  2:1436023/1‑140 (MQ=255)                                                           tCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCAc                                                                             >  2:381387/1‑140 (MQ=255)                                                                  gCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGAtt                                                                      <  1:268229/140‑1 (MQ=255)                                                                      gCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACg                                                                            <  1:75044/130‑1 (MQ=255)                                                                      gCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACg                                                                            >  2:75044/1‑130 (MQ=255)                                                                       cGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaaa                                                                 >  2:902787/1‑140 (MQ=255)                                                                           aTCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGa                                                             >  2:199453/1‑140 (MQ=255)                                                                              cGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAAcc                                                          <  2:255076/140‑1 (MQ=255)                                                                                 aTCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAAt                                                                <  1:52325/131‑1 (MQ=255)                                                                                 aTCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAAt                                                                >  2:52325/1‑131 (MQ=255)                                                                                 aTCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGc                                                       >  1:535167/1‑140 (MQ=255)                                                                                  tCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc                                                      <  1:1436023/140‑1 (MQ=255)                                                                                  tCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc                                                      <  2:1233282/140‑1 (MQ=255)                                                                                  tCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc                                                      <  1:476710/140‑1 (MQ=255)                                                                                  tCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc                                                      <  1:71899/140‑1 (MQ=255)                                                                                    aGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGa                                                    >  2:1239162/1‑140 (MQ=255)                                                                                      gTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAGACCACGCTGATTTAAAATCGAACCAGCCGAgg                                                  >  2:322134/1‑140 (MQ=255)                                                                                      gTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAgg                                                  <  2:143387/140‑1 (MQ=255)                                                                                       ttCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg                                                 <  1:1278427/140‑1 (MQ=255)                                                                                                tATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc                                                      >  2:837290/1‑126 (MQ=255)                                                                                                tATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc                                                      <  1:837290/126‑1 (MQ=255)                                                                                                tATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATgc                                        >  1:35670/1‑140 (MQ=255)                                                                                                tATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATgc                                        >  2:887711/1‑140 (MQ=255)                                                                                                tATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATgc                                        >  1:820277/1‑140 (MQ=255)                                                                                                    ttCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAg                                    <  1:781963/140‑1 (MQ=255)                                                                                                        cAGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcg                                >  2:394459/1‑140 (MQ=255)                                                                                                         aGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc                               >  2:894967/1‑140 (MQ=255)                                                                                                         aGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc                               >  2:48407/1‑140 (MQ=255)                                                                                                         aGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc                               <  1:887711/140‑1 (MQ=255)                                                                                                         aGCGCACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc                               >  2:937041/1‑140 (MQ=255)                                                                                                           cgcACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAg                                    >  2:1311125/1‑133 (MQ=255)                                                                                                           cgcACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAg                                    <  1:1311125/133‑1 (MQ=255)                                                                                                             cACGCGTACGCGTGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCAt                           <  2:681651/140‑1 (MQ=255)                                                                                                               cgcgtacgcgtGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATAc                         >  2:162381/1‑140 (MQ=255)                                                                                                               cgcgtacgcgtGGCACCCCGTTGAGAGAGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATAc                         <  2:873027/140‑1 (MQ=255)                                                                                                                 cgtacgcgtGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTg                       <  1:937041/140‑1 (MQ=255)                                                                                                                   tacgcgtGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAGTCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCg                     <  2:1294616/140‑1 (MQ=255)                                                                                                                      gcgtGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTg                  <  1:48407/140‑1 (MQ=255)                                                                                                                      gcgtGGCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTg                  <  1:894967/140‑1 (MQ=255)                                                                                                                          ggCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGcccc              >  1:651360/1‑140 (MQ=255)                                                                                                                          ggCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGcccc              >  1:886369/1‑140 (MQ=255)                                                                                                                          ggCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGcccc              >  1:1387270/1‑140 (MQ=255)                                                                                                                           gCACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCt             <  2:651360/140‑1 (MQ=255)                                                                                                                            cACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCg                     >  2:1312909/1‑131 (MQ=255)                                                                                                                            cACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCg                     <  1:1312909/131‑1 (MQ=255)                                                                                                                            cACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg            <  1:503590/140‑1 (MQ=255)                                                                                                                            cACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg            <  1:270222/140‑1 (MQ=255)                                                                                                                            cACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg            >  1:1093584/1‑140 (MQ=255)                                                                                                                            cACCCCGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg            <  2:1093584/140‑1 (MQ=255)                                                                                                                                ccGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATg        >  1:1249675/1‑140 (MQ=255)                                                                                                                                 cGTTGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGa       <  2:330021/140‑1 (MQ=255)                                                                                                                                   ttGAGAGCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATc     >  2:490495/1‑140 (MQ=255)                                                                                                                                      agagCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAGACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc  <  1:322134/140‑1 (MQ=255)                                                                                                                                      agagCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc  <  1:1076402/140‑1 (MQ=255)                                                                                                                                      agagCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc  <  1:221394/140‑1 (MQ=255)                                                                                                                                      agagCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc  <  1:389509/140‑1 (MQ=255)                                                                                                                                      agagCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc  <  1:506816/140‑1 (MQ=255)                                                                                                                                      agagCGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc  <  2:1249675/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGC  >  NC_000913/75471‑75743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑