Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,365,094	A→G	Y288C (TAC→TGC)	puuB →	gamma‑glutamylputrescine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,365,094	0	A	G	100.0%	151.8 / NA	47	Y288C (TAC→TGC)	puuB	gamma‑glutamylputrescine oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/23);  total (24/23)

CGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGAT  >  NC_000913/1364962‑1365230                                                                                                                                     |                                                                                                                                         cGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCtt                                                                                                                                   >  1:53997/1‑140 (MQ=255)  ggAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTa                                                                                                                                  >  1:560113/1‑140 (MQ=255)      cTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTAccgc                                                                                                                              <  1:818873/140‑1 (MQ=255)          cGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGAc                                                                                                                          >  1:222663/1‑140 (MQ=255)            aaaCGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACaa                                                                                                                        <  2:535850/140‑1 (MQ=255)              aCGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAAcc                                                                                                                      >  1:1147991/1‑140 (MQ=255)                gcagcaTGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGc                                                                                                                    <  2:1036892/140‑1 (MQ=255)                   gcaTGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCctg                                                                                                                 <  1:571217/140‑1 (MQ=255)                           tGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTAcgg                                                                                                         >  1:1153333/1‑140 (MQ=255)                                  cGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATTTGCTTGATTACTGCCGCCTTACCGCCGACAACCGCCTGCTGTACggcggcggc                                                                                                  <  2:1060431/140‑1 (MQ=255)                                  cGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACggcggcggc                                                                                                  <  1:984841/140‑1 (MQ=255)                                  cGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACggcggcggc                                                                                                  <  2:222663/140‑1 (MQ=255)                                  cGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACggcggcggc                                                                                                  <  1:1369706/140‑1 (MQ=255)                                    cAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGt                                                                                                <  2:560113/140‑1 (MQ=255)                                     aGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGACCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTg                                                                                               >  2:1073941/1‑140 (MQ=255)                                               caccGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGgcg                                                                                     >  1:970302/1‑140 (MQ=255)                                                 ccGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGgcgcg                                                                                   >  1:1391907/1‑140 (MQ=255)                                                   gAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGgcgcgcg                                                                                 <  2:970302/140‑1 (MQ=255)                                                   gAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGgcgcgcg                                                                                 <  1:123283/140‑1 (MQ=255)                                                    aaCGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGgcgcgcgc                                                                                >  2:680091/1‑140 (MQ=255)                                                    aaCGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGgcgcgcgc                                                                                >  1:1300256/1‑140 (MQ=255)                                                            tCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGa                                                                        <  1:1238331/140‑1 (MQ=255)                                                                     ttAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGa                                                               <  2:1153333/140‑1 (MQ=255)                                                                     ttAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGa                                                               <  2:208263/140‑1 (MQ=255)                                                                     ttAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGa                                                               <  1:1190787/140‑1 (MQ=255)                                                                      tAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAg                                                              <  2:1391907/140‑1 (MQ=255)                                                                        gCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAgcg                                                            <  2:53997/140‑1 (MQ=255)                                                                             ttCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTtg                                                       >  1:1350780/1‑140 (MQ=255)                                                                             ttCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTtg                                                       >  1:912176/1‑140 (MQ=255)                                                                               cGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTtgtg                                                     >  1:331511/1‑140 (MQ=255)                                                                                      cccGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGa                                              <  2:331511/140‑1 (MQ=255)                                                                                         gAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTTCTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAAc                                           <  2:115250/140‑1 (MQ=255)                                                                                          aaaaaCTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAAct                                          >  2:792198/1‑140 (MQ=255)                                                                                              actactGTGTGGAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAActgctg                                      >  1:611880/1‑140 (MQ=255)                                                                                                        ggAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTcc                            <  2:611880/140‑1 (MQ=255)                                                                                                         gAAGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTccc                           >  2:655860/1‑140 (MQ=255)                                                                                                          aaGACTGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCg                          <  2:623249/140‑1 (MQ=255)                                                                                                               tGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAGAACCTTcc                            >  2:1455030/1‑133 (MQ=255)                                                                                                               tGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAGAACCTTcc                            <  1:1455030/133‑1 (MQ=255)                                                                                                               tGTAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCt                     >  2:789572/1‑140 (MQ=255)                                                                                                                 tAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTcc                            <  2:506307/131‑1 (MQ=255)                                                                                                                 tAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTcc                            >  1:506307/1‑131 (MQ=255)                                                                                                                 tAACTATCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGa                   >  1:56429/1‑140 (MQ=255)                                                                                                                       tCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTcc                            <  1:504373/125‑1 (MQ=255)                                                                                                                       tCTGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTcc                            >  2:504373/1‑125 (MQ=255)                                                                                                                         tGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCg                          <  1:761228/125‑1 (MQ=255)                                                                                                                         tGCTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCg                          >  2:761228/1‑125 (MQ=255)                                                                                                                           cTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCg                          >  1:300969/1‑123 (MQ=255)                                                                                                                           cTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCg                          <  2:300969/123‑1 (MQ=255)                                                                                                                           cTTGATTACTGCCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGaa         >  1:894694/1‑140 (MQ=255)                                                                                                                                 tactgcCGTCTTACCGCCGCCAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGa   >  2:1182897/1‑140 (MQ=255)                                                                                                                                 tactgcCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGa   >  2:512385/1‑140 (MQ=255)                                                                                                                                  actgcCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGAt  <  1:680091/140‑1 (MQ=255)                                                                                                                                  actgcCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGAt  <  1:1073941/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CGGAACTGGCGAAACGCAGCATGCCGTGCGGCACGCAGGTGATCACCACCGAACGGCTGTCGGAAGATTTAGCCCGTTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTATCTGCTTGATTACTACCGTCTTACCGCCGACAACCGCCTGCTGTACGGCGGCGGCGTGGTCTACGGCGCGCGCGACCCGGATGACGTTGAGCGCCTTGTGGTGCCGAAACTGCTGAAAACCTTCCCGCAGCTGAAGGGCGTGAAAATTGAT  >  NC_000913/1364962‑1365230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑