Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,475,398	T→C	S85S (AGT→AGC)	ydbD →	DUF2773 domain‑containing protein YdbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,475,398	0	T	C	100.0%	197.8 / NA	57	S85S (AGT→AGC)	ydbD	DUF2773 domain‑containing protein YdbD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

GCTACCGCTGAAGATAAACAACAAGTTGAACAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGTAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACC  >  NC_000913/1475267‑1475533                                                                                                                                    |                                                                                                                                        gCTACCGCTGAAGATAAACAACAAGTTGAACAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCaaaa                                                                                                                                 <  2:1025941/140‑1 (MQ=255)            aGATAAACAACAAGTTGAACAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTc                                                                                                                      >  2:1400341/1‑140 (MQ=255)                  acaacaAGTTGAACAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGa                                                                                                                >  1:1469029/1‑140 (MQ=255)                             aaCAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTc                                                                                                     <  1:258812/140‑1 (MQ=255)                             aaCAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTc                                                                                                     <  2:983140/140‑1 (MQ=255)                                 aGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAg                                                                                                 >  2:595586/1‑140 (MQ=255)                                  gCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAgg                                                                                                >  2:1179862/1‑140 (MQ=255)                                   cTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGt                                                                                               <  1:1400341/140‑1 (MQ=255)                                          cAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATAttt                                                                                        >  1:31206/1‑140 (MQ=255)                                             tAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAGAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCa                                                                                     >  2:110009/1‑140 (MQ=255)                                             tAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCa                                                                                     >  1:752779/1‑140 (MQ=255)                                              aGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCAc                                                                                    >  2:209120/1‑140 (MQ=255)                                                cGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAGGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACtt                                                                                  >  2:1007093/1‑140 (MQ=255)                                                cGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACtt                                                                                  >  2:700157/1‑140 (MQ=255)                                                cGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACtt                                                                                  >  2:1403183/1‑140 (MQ=255)                                                cGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACtt                                                                                  >  1:388264/1‑140 (MQ=255)                                                cGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACtt                                                                                  >  1:483522/1‑140 (MQ=255)                                                cGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACtt                                                                                  >  1:476394/1‑140 (MQ=255)                                                  tGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtc                                                                                >  2:45428/1‑140 (MQ=255)                                                    aaTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtctc                                                                              <  2:1438595/140‑1 (MQ=255)                                                    aaTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtctc                                                                              >  2:693738/1‑140 (MQ=255)                                                    aaTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtctc                                                                              >  2:1366518/1‑140 (MQ=255)                                                    aaTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTcctc                                                                              <  2:388264/140‑1 (MQ=255)                                                     aTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtctct                                                                             <  2:476394/140‑1 (MQ=255)                                                     aTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtctct                                                                             <  1:29864/140‑1 (MQ=255)                                                     aTCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTtctct                                                                             <  1:1206993/140‑1 (MQ=255)                                                       cTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTctctct                                                                           >  2:1389535/1‑140 (MQ=255)                                                                ttCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATcc                                                                  <  1:826869/140‑1 (MQ=255)                                                                ttCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATcc                                                                  <  1:209120/140‑1 (MQ=255)                                                                ttCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATcc                                                                  <  2:1203625/140‑1 (MQ=255)                                                                          cTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTAtt                                                        <  2:1099646/140‑1 (MQ=255)                                                                           tGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCa                                                                 >  2:37544/1‑130 (MQ=255)                                                                           tGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCa                                                                 <  1:37544/130‑1 (MQ=255)                                                                                            tGCATCGTGGCGATTTAGTGGTAGAAGGTAATGTAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCa                                      >  2:146946/1‑140 (MQ=255)                                                                                            tGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCa                                      <  1:700157/140‑1 (MQ=255)                                                                                            tGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCa                                      >  1:872896/1‑140 (MQ=255)                                                                                            tGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCa                                      >  2:573624/1‑140 (MQ=255)                                                                                            tGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTCTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGc                                       <  2:54542/139‑1 (MQ=255)                                                                                            tGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTCTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGc                                       >  1:54542/1‑139 (MQ=255)                                                                                               aTCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACg                                   >  2:693054/1‑140 (MQ=255)                                                                                                      cGATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAg                            >  2:62145/1‑140 (MQ=255)                                                                                                       gATTTAGTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGt                           <  1:110009/140‑1 (MQ=255)                                                                                                          ttAGTGGTAGAAGGTAATGTAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGc                        <  1:146946/140‑1 (MQ=255)                                                                                                             gTGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGa                     >  2:996226/1‑140 (MQ=255)                                                                                                              tGGTAGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGAt                    >  1:461737/1‑140 (MQ=255)                                                                                                                  aGAAGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTcac                <  1:227514/140‑1 (MQ=255)                                                                                                                    aaGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTcacca              <  1:595586/140‑1 (MQ=255)                                                                                                                    aaGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTcacca              <  1:45428/140‑1 (MQ=255)                                                                                                                    aaGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTcacca              <  2:690008/140‑1 (MQ=255)                                                                                                                     aGGTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTcaccac             <  2:31206/140‑1 (MQ=255)                                                                                                                      ggTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACt            >  2:443506/1‑140 (MQ=255)                                                                                                                       gTAATATAGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTg           <  1:1403183/140‑1 (MQ=255)                                                                                                                           tataGAGAGCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTaa       >  1:117253/1‑140 (MQ=255)                                                                                                                                agagCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGtt         <  2:421075/133‑1 (MQ=255)                                                                                                                                agagCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGtt         >  1:421075/1‑133 (MQ=255)                                                                                                                                agagCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTAcc  >  2:122945/1‑140 (MQ=255)                                                                                                                                agagCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTAcc  >  1:392422/1‑140 (MQ=255)                                                                                                                                agagCAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTAcc  >  2:920005/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        GCTACCGCTGAAGATAAACAACAAGTTGAACAAGCTATCAACAGTAGCGTGAATCTTGTCCCCTTCGGTTTATCTGCATCGAACTGGAAAGTGCATCGTGGCGATTTAGTGGTAGAAGGTAATATAGAGAGTAATCAAAAATTGATTGTTCTTGGGAATTTGACAGTCAAAGGTAATATTTCCACTTTCTCTCTTTCTAATCCATGGGTTATTCTCGGTAACGTGACGGCAACGAATATAGTTGCGGATTCACCACTGTTAATTACC  >  NC_000913/1475267‑1475533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑