Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,861	A→G	W244R (TGG→CGG)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,861	0	A	G	100.0%	250.8 / NA	73	W244R (TGG→CGG)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/33);  total (40/33)

GCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAG  >  NC_000913/1541725‑1541995                                                                                                                                         |                                                                                                                                       gCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTc                                                                                                                                     <  1:121056/140‑1 (MQ=255)     cTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCa                                                                                                                                 <  1:1307709/140‑1 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:932982/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:451934/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:389364/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  1:1116131/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:165356/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:111898/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:1046450/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  1:737997/1‑140 (MQ=255)                tCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTATGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTAc                                                                                                                      >  2:723072/1‑140 (MQ=255)                  aGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGt                                                                                                                    >  1:1238762/1‑140 (MQ=255)                    gggTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCg                                                                                                                  >  1:24489/1‑140 (MQ=255)                         aTGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACTGGTCGGCAGATTCCGGTACGTCGGTTTg                                                                                                             >  2:187711/1‑140 (MQ=255)                         aTGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTg                                                                                                             >  1:1055017/1‑140 (MQ=255)                          tGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGc                                                                                                            <  1:767553/140‑1 (MQ=255)                           ggCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACTGGTCGGCAGATTCCGGTACGTCGGTTTGCt                                                                                                           <  1:187711/140‑1 (MQ=255)                               aTGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGcc                                                                                                       >  2:1471805/1‑140 (MQ=255)                                       aGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCa                                                                                               <  1:111898/140‑1 (MQ=255)                                           ccTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCgg                                                                                           >  2:1105336/1‑140 (MQ=255)                                            cTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGc                                                                                          >  1:337457/1‑140 (MQ=255)                                                   gCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAggcgg                                                                                   <  1:389364/140‑1 (MQ=255)                                                   gCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTATGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAggcgg                                                                                   <  1:723072/140‑1 (MQ=255)                                                        cTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGggcggc                                                                              >  1:224111/1‑140 (MQ=255)                                                         ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa                                                                             <  2:737997/140‑1 (MQ=255)                                                         ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa                                                                             >  1:542028/1‑140 (MQ=255)                                                         ttCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCa                                                                             <  1:536666/140‑1 (MQ=255)                                                           cGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAgg                                                                           >  1:516049/1‑140 (MQ=255)                                                             gTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTc                                                                         <  2:516049/140‑1 (MQ=255)                                                             gTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACCTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTc                                                                         <  2:1238762/140‑1 (MQ=255)                                                              tAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCg                                                                        <  2:403260/140‑1 (MQ=255)                                                              tAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCg                                                                        <  2:542028/140‑1 (MQ=255)                                                                aagaagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCa                                                                      >  1:134320/1‑140 (MQ=255)                                                                   aagTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATa                                                                   >  2:1244997/1‑140 (MQ=255)                                                                    agTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATAc                                                                  <  1:1105336/140‑1 (MQ=255)                                                                    agTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATAc                                                                  <  1:165356/140‑1 (MQ=255)                                                                        ggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAg                                                               <  2:557049/139‑1 (MQ=255)                                                                        ggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAg                                                               >  1:557049/1‑139 (MQ=255)                                                                        ggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGt                                                              >  2:248503/1‑140 (MQ=255)                                                                        ggCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGt                                                              >  1:715899/1‑140 (MQ=255)                                                                          cGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAg                                                               >  2:299489/1‑137 (MQ=255)                                                                          cGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAg                                                               <  1:299489/137‑1 (MQ=255)                                                                          cGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCg                                                            >  1:171385/1‑140 (MQ=255)                                                                            tCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCGATACCAGTCGTa                                                          <  1:932982/140‑1 (MQ=255)                                                                            tCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTa                                                          <  2:1116131/140‑1 (MQ=255)                                                                            tCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTa                                                          <  2:337457/140‑1 (MQ=255)                                                                             ccGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAg                                                         >  2:1081565/1‑140 (MQ=255)                                                                             ccGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAg                                                         >  2:1172937/1‑140 (MQ=255)                                                                                      gtgtCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTaaa                                                <  1:1471805/140‑1 (MQ=255)                                                                                          cTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAgg                                            <  2:224111/140‑1 (MQ=255)                                                                                           tGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAggg                                           >  2:118837/1‑139 (MQ=255)                                                                                                tACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTgccgc                                      <  1:1244997/140‑1 (MQ=255)                                                                                                  cGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGGGCCGCCa                                    >  2:1390102/1‑140 (MQ=255)                                                                                                    ttAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAag                                  <  1:248503/140‑1 (MQ=255)                                                                                                    ttAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAag                                  <  2:171385/140‑1 (MQ=255)                                                                                                    ttAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAag                                  >  1:370240/1‑140 (MQ=255)                                                                                                     tAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCa                                    >  2:588180/1‑137 (MQ=255)                                                                                                     tAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCa                                    <  1:588180/137‑1 (MQ=255)                                                                                                        aCCCCCAGGCGATGATGTAGCTAGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAagcagc                              <  1:1046450/140‑1 (MQ=255)                                                                                                          ccccAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGa                            <  1:118837/140‑1 (MQ=255)                                                                                                            ccAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACa                          >  2:323260/1‑140 (MQ=255)                                                                                                            ccAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACa                          >  2:1067680/1‑140 (MQ=255)                                                                                                            ccAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACa                          >  1:129884/1‑140 (MQ=255)                                                                                                             cAGGCGATGATGTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAg                         >  1:494973/1‑140 (MQ=255)                                                                                                                       tgTAGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTAGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGtt               >  1:1233471/1‑140 (MQ=255)                                                                                                                          aGCTGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcg            >  1:1104176/1‑140 (MQ=255)                                                                                                                             tGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcggcg         <  1:1172937/140‑1 (MQ=255)                                                                                                                             tGGAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcggcg         <  1:1081565/140‑1 (MQ=255)                                                                                                                              ggAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcggcgg        >  2:167000/1‑140 (MQ=255)                                                                                                                              ggAGTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcggcgg        <  2:494973/140‑1 (MQ=255)                                                                                                                                 gTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcggcgg        <  2:142437/137‑1 (MQ=255)                                                                                                                                 gTTATACCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCcggcgg        >  1:142437/1‑137 (MQ=255)                                                                                                                                    ataCCGGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAg  <  1:323260/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTTAGTGCCTTTGTAGCGTACTTCGGTAAAGAAGTGGGCGTCCGGCGTACGTGTCTGCGGTACGTTAGACCCCCAGGCGATGATGTAGCTGGAGTTATACCAGTCGGCAGATTCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGTCGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAG  >  NC_000913/1541725‑1541995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑