Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,603,810 T→C G264G (GGT→GGC)  lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,8100TC100.0% 177.1 / NA 52G264G (GGT→GGC) lsrCAutoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/29);  total (23/29)

GCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTG  >  NC_000913/1603679‑1603946
                                                                                                                                   |                                                                                                                                        
gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACt                                                                                                                                  <  1:552111/140‑1 (MQ=255)
     aaTTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGgcg                                                                                                                             <  2:722702/140‑1 (MQ=255)
          tgtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATg                                                                                                                         >  1:263188/1‑139 (MQ=255)
          tgtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATg                                                                                                                         <  2:263188/139‑1 (MQ=255)
                  tCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGAc                                                                                                                >  1:998973/1‑140 (MQ=255)
                   cGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACg                                                                                                               <  1:1071158/140‑1 (MQ=255)
                   cGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACg                                                                                                               <  2:1077957/140‑1 (MQ=255)
                    gCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGc                                                                                                              >  2:812772/1‑140 (MQ=255)
                       gATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCA‑TAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGAt                                                                                                          <  1:795103/140‑1 (MQ=255)
                          tGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCg                                                                                                        <  1:1322892/140‑1 (MQ=255)
                              tttATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAg                                                                                                    <  1:178031/140‑1 (MQ=255)
                                  tCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTa                                                                                                >  2:879378/1‑140 (MQ=255)
                                   ccccAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTAc                                                                                               <  1:185594/140‑1 (MQ=255)
                                      cAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTgg                                                                                            >  1:245884/1‑140 (MQ=255)
                                         ccAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGc                                                                                         >  2:395048/1‑140 (MQ=255)
                                             aCCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt                                                                                     >  1:669239/1‑140 (MQ=255)
                                                ggTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTgcg                                                                                  <  2:245884/140‑1 (MQ=255)
                                                    ccGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCAtt                                                                              <  1:740714/140‑1 (MQ=255)
                                                       ggCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCg                                                                           <  1:395048/140‑1 (MQ=255)
                                                         cTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGc                                                                         <  1:812772/140‑1 (MQ=255)
                                                           ggAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCAt                                                                       >  2:225840/1‑140 (MQ=255)
                                                            gagaTGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCAtg                                                                      >  2:1089452/1‑140 (MQ=255)
                                                                      cAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGAt                                                            >  1:66973/1‑140 (MQ=255)
                                                                            cAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATc                                                      >  2:220210/1‑140 (MQ=255)
                                                                             aGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATcg                                                     <  1:879378/140‑1 (MQ=255)
                                                                              gCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATcgc                                                    <  1:220210/140‑1 (MQ=255)
                                                                               ccTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATcgcg                                                   >  2:978386/1‑140 (MQ=255)
                                                                                 tGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCggg                                                 <  1:1460946/140‑1 (MQ=255)
                                                                                  gCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGt                                                <  2:998973/140‑1 (MQ=255)
                                                                                   cGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTc                                               >  1:1019553/1‑140 (MQ=255)
                                                                                   cGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTc                                               <  1:995087/140‑1 (MQ=255)
                                                                                           gcggcATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTctg                                       >  2:1440573/1‑140 (MQ=255)
                                                                                            cggcATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTctgc                                      >  1:53993/1‑140 (MQ=255)
                                                                                               cATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTgg                                   <  1:1089452/140‑1 (MQ=255)
                                                                                                    gTTTGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTg                              >  2:1133585/1‑140 (MQ=255)
                                                                                                     tttGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGc                             <  1:1133585/140‑1 (MQ=255)
                                                                                                     tttGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGc                             <  1:345928/140‑1 (MQ=255)
                                                                                                     tttGCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGc                             >  1:1231155/1‑140 (MQ=255)
                                                                                                        gCTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTgg                          <  2:1231155/140‑1 (MQ=255)
                                                                                                         cTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCt                            <  1:606141/137‑1 (MQ=255)
                                                                                                         cTCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCt                            >  2:606141/1‑137 (MQ=255)
                                                                                                          tCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGgtg                        <  2:1019553/140‑1 (MQ=255)
                                                                                                          tCGGTGGTTCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGACAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGgtg                        >  1:38870/1‑140 (MQ=255)
                                                                                                                 ttCCGGTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATg                 <  1:792140/140‑1 (MQ=255)
                                                                                                                      gTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATg                 <  1:995168/135‑1 (MQ=255)
                                                                                                                      gTGCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATg                 >  2:995168/1‑135 (MQ=255)
                                                                                                                        gCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGgtg                        >  1:1422223/1‑126 (MQ=255)
                                                                                                                        gCGATCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGgtg                        <  2:1422223/126‑1 (MQ=255)
                                                                                                                           aTCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTTATGGGAGCCTggg       >  2:1101764/1‑138 (MQ=255)
                                                                                                                           aTCATTGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCg       >  1:390025/1‑140 (MQ=255)
                                                                                                                               ttGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTtgt   <  2:390025/140‑1 (MQ=255)
                                                                                                                                tGGCGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTtgtg  <  1:978386/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                        
GCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTG  >  NC_000913/1603679‑1603946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑