Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,784	T→C	F188L (TTC→CTC)	rsmF →	16S rRNA m(5)C1407 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,784	0	T	C	100.0%	334.2 / NA	96	F188L (TTC→CTC)	rsmF	16S rRNA m(5)C1407 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/44);  total (52/44)

AAGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCT  >  NC_000913/1920647‑1920919                                                                                                                                          |                                                                                                                                        aaGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTc                                                                                                                                       >  2:1265879/1‑140 (MQ=255) aaGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTc                                                                                                                                       >  1:186814/1‑140 (MQ=255) aaGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTc                                                                                                                                       >  2:878772/1‑140 (MQ=255) aaGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTc                                                                                                                                       >  1:364095/1‑140 (MQ=255) aaGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGGAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATAAGTAATGTTGAGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTc                                                                                                                                       >  2:1028968/1‑140 (MQ=255)   ggggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGa                                                                                                                                     <  1:523433/140‑1 (MQ=255)    gggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGAt                                                                                                                                    >  1:59075/1‑140 (MQ=255)    gggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTCGGTGCGGCAGTGCCAGAAATGCTCGAt                                                                                                                                    >  2:1156962/1‑140 (MQ=255)     ggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGa                                                                                                                                     >  1:363202/1‑138 (MQ=255)     ggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGa                                                                                                                                     <  2:363202/138‑1 (MQ=255)     ggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATg                                                                                                                                   >  1:830993/1‑140 (MQ=255)     ggCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATg                                                                                                                                   <  1:1265879/140‑1 (MQ=255)       cAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATTGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGcc                                                                                                                                 >  1:976282/1‑140 (MQ=255)       cAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGcc                                                                                                                                 >  2:1459307/1‑140 (MQ=255)          tCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCAtt                                                                                                                              >  2:333715/1‑140 (MQ=255)              tGCCAATGAGTTTTCCGCCAGTCTGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGc                                                                                                                          >  2:428251/1‑140 (MQ=255)              tGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGc                                                                                                                          >  1:1027341/1‑140 (MQ=255)               gCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCt                                                                                                                         >  1:1257943/1‑140 (MQ=255)                  aaTGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGa                                                                                                                      <  1:772762/140‑1 (MQ=255)                   aTGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGAc                                                                                                                     <  2:1257943/140‑1 (MQ=255)                   aTGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGAc                                                                                                                     <  1:370628/140‑1 (MQ=255)                    tGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGACATATTGCTGGTCg                                                                                                                    >  2:906767/1‑140 (MQ=255)                        ttttCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTcc                                                                                                                <  2:186814/140‑1 (MQ=255)                        ttttCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTcc                                                                                                                <  2:59075/140‑1 (MQ=255)                          ttCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTcc                                                                                                                <  2:752978/138‑1 (MQ=255)                          ttCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTcc                                                                                                                >  1:752978/1‑138 (MQ=255)                             cGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGct                                                                                                           <  1:1459307/140‑1 (MQ=255)                             cGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGct                                                                                                           <  1:1430288/140‑1 (MQ=255)                             cGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTCGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGct                                                                                                           <  1:1156962/140‑1 (MQ=255)                                   tCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCg                                                                                                     <  2:364095/140‑1 (MQ=255)                                         aaaaGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATTGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCg                                                                                               <  2:976282/140‑1 (MQ=255)                                          aaaGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgt                                                                                              <  1:906767/140‑1 (MQ=255)                                           aaGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgtg                                                                                             <  2:1027341/140‑1 (MQ=255)                                           aaGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgtg                                                                                             <  2:64062/140‑1 (MQ=255)                                             gtgtTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgtggt                                                                                           >  1:1173896/1‑140 (MQ=255)                                             gtgtTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgt                                                                                              <  2:385308/137‑1 (MQ=255)                                             gtgtTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgt                                                                                              >  1:385308/1‑137 (MQ=255)                                                ttACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTgg                                                                                                       <  2:698679/125‑1 (MQ=255)                                                ttACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTgg                                                                                                       >  1:698679/1‑125 (MQ=255)                                                ttACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGc                                                                                                >  1:452748/1‑132 (MQ=255)                                                ttACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGc                                                                                                <  2:452748/132‑1 (MQ=255)                                                 tACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGt                                                                                       >  1:500000/1‑140 (MQ=255)                                                  aCATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTa                                                                                      <  1:621569/140‑1 (MQ=255)                                                    aTGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTaaa                                                                                    <  2:1417691/140‑1 (MQ=255)                                                      gCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGc                                                                                         <  2:591571/133‑1 (MQ=255)                                                      gCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGc                                                                                         >  1:591571/1‑133 (MQ=255)                                                       ccAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGACGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGAt                                                                                 >  1:442791/1‑140 (MQ=255)                                                        cAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATc                                                                                >  2:670176/1‑140 (MQ=255)                                                           tatCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCg                                                                             >  1:1229790/1‑140 (MQ=255)                                                                gCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATgcg                                                                        >  1:1170233/1‑140 (MQ=255)                                                                    cTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaa                                                                    >  1:177771/1‑140 (MQ=255)                                                                    cTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaa                                                                    >  2:472524/1‑140 (MQ=255)                                                                     tgtgGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaa                                                                   >  1:766390/1‑140 (MQ=255)                                                                     tgtgGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaa                                                                   >  2:1174195/1‑140 (MQ=255)                                                                     tgtgGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaa                                                                   >  1:363185/1‑140 (MQ=255)                                                                        ggTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaaaaa                                                                <  2:1229790/140‑1 (MQ=255)                                                                               gTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCa                                                         >  2:1161427/1‑140 (MQ=255)                                                                                   tGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAg                                                     >  2:104357/1‑140 (MQ=255)                                                                                   tGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAg                                                     <  2:177771/140‑1 (MQ=255)                                                                                     ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCTAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGt                                                           <  1:1074511/132‑1 (MQ=255)                                                                                     ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCTAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGt                                                           >  2:1074511/1‑132 (MQ=255)                                                                                      tGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGaaa                                                  <  1:472524/140‑1 (MQ=255)                                                                                        cgcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGc                                                >  2:392691/1‑140 (MQ=255)                                                                                        cgcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGc                                                >  1:649252/1‑140 (MQ=255)                                                                                          cTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGc                                                >  2:913463/1‑138 (MQ=255)                                                                                          cTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGc                                                <  1:913463/138‑1 (MQ=255)                                                                                          cTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCaa                                              >  1:1273441/1‑140 (MQ=255)                                                                                          cTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCaa                                              >  2:324612/1‑140 (MQ=255)                                                                                          cTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCaa                                              >  2:621425/1‑140 (MQ=255)                                                                                          cTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTACCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCaa                                              >  1:374271/1‑140 (MQ=255)                                                                                           tCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTACCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAAt                                             <  2:374271/140‑1 (MQ=255)                                                                                                   tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  1:1161427/140‑1 (MQ=255)                                                                                                   tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  1:1028968/140‑1 (MQ=255)                                                                                                   tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  1:428251/140‑1 (MQ=255)                                                                                                   tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  1:392691/140‑1 (MQ=255)                                                                                                   tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  1:324612/140‑1 (MQ=255)                                                                                                     tGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCg                                   <  2:1173896/140‑1 (MQ=255)                                                                                                       aTGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCa                                 <  1:574992/140‑1 (MQ=255)                                                                                                       aTGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAACGCa                                 >  1:432357/1‑140 (MQ=255)                                                                                                         ggCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  2:798818/134‑1 (MQ=255)                                                                                                         ggCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     >  1:798818/1‑134 (MQ=255)                                                                                                           ccGCGTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTa                             >  1:1312393/1‑140 (MQ=255)                                                                                                             gcgTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     <  2:253872/130‑1 (MQ=255)                                                                                                             gcgTGTTTGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAAt                                     >  1:253872/1‑130 (MQ=255)                                                                                                                    tGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACggg                    <  1:670176/140‑1 (MQ=255)                                                                                                                    tGGTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACggg                    <  2:1273441/140‑1 (MQ=255)                                                                                                                      gTGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAg                  <  2:766390/140‑1 (MQ=255)                                                                                                                       tGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACggg                    <  2:553897/137‑1 (MQ=255)                                                                                                                       tGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACggg                    >  1:553897/1‑137 (MQ=255)                                                                                                                       tGCGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGc                 <  2:830993/140‑1 (MQ=255)                                                                                                                         cGGCAGTGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCtt               >  1:61898/1‑140 (MQ=255)                                                                                                                               tGCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGAc         >  2:1045364/1‑140 (MQ=255)                                                                                                                                gCCAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACa        >  1:1396021/1‑140 (MQ=255)                                                                                                                                 ccAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGa          >  1:822337/1‑137 (MQ=255)                                                                                                                                 ccAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGa          <  2:822337/137‑1 (MQ=255)                                                                                                                                 ccAGAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAg       >  2:1243389/1‑140 (MQ=255)                                                                                                                                    gAAATGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAAGAAATCGCAGCTCCACAACGGGAGCTTATCGACAgcgc    <  1:104357/140‑1 (MQ=255)                                                                                                                                      aaTGCTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCt  >  1:1416351/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AAGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCT  >  NC_000913/1920647‑1920919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑