| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 984,199 | (G)5→6 | coding (550/648 nt) | gloC → | hydroxyacylglutathione hydrolase GloC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 984,194 | 1 | . | G | 97.4% | 136.9 / ‑3.1 | 39 | coding (545/648 nt) | gloC | hydroxyacylglutathione hydrolase GloC |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (13/25); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACT‑GGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGC > NC_000913/984057‑984325 | tGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACT‑gg > 2:583204/1‑140 (MQ=255) ttttttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACT‑ggg > 2:873398/1‑134 (MQ=255) ttttttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACT‑ggg < 1:873398/134‑1 (MQ=255) ttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGt < 2:689254/140‑1 (MQ=255) gCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTc < 2:411040/140‑1 (MQ=255) gCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTc < 2:366109/140‑1 (MQ=255) aaGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATC‑AAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCggg < 1:828807/140‑1 (MQ=255) aGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCggg < 1:547752/140‑1 (MQ=255) ctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACg > 1:1161490/1‑140 (MQ=255) ctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACg < 1:79094/140‑1 (MQ=255) tgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACgg > 2:55027/1‑140 (MQ=255) tttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGAcc < 1:438480/140‑1 (MQ=255) tttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGAcc < 1:871833/140‑1 (MQ=255) tttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGAcc < 1:55027/140‑1 (MQ=255) tttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGAcc < 1:589697/140‑1 (MQ=255) tttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGAACATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGAcc < 2:500497/140‑1 (MQ=255) ttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCa < 2:612594/140‑1 (MQ=255) ttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCa < 1:129493/140‑1 (MQ=255) ttCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCa < 1:320607/140‑1 (MQ=255) ttATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGa > 2:406736/1‑126 (MQ=255) ttATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGa < 1:406736/126‑1 (MQ=255) aaaGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATcc > 1:485584/1‑127 (MQ=255) aaaGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATcc < 2:485584/127‑1 (MQ=255) aaaGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg > 2:1061489/1‑140 (MQ=255) aGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACT‑GGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAAc > 1:503128/1‑140 (MQ=255) cGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc < 2:1161490/140‑1 (MQ=255) ggAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGcc < 2:694825/140‑1 (MQ=255) tAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCa < 2:242161/140‑1 (MQ=255) aGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc < 2:410227/127‑1 (MQ=255) aGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc > 1:410227/1‑127 (MQ=255) aGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCt > 1:1140275/1‑140 (MQ=255) ggCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGaaa < 1:995384/140‑1 (MQ=255) gCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAAt > 2:119657/1‑140 (MQ=255) gCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAAt > 1:32947/1‑140 (MQ=255) aaCTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTa < 1:723082/140‑1 (MQ=255) aaCTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTa < 1:1002659/140‑1 (MQ=255) cTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACGCTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAg > 2:1029076/1‑140 (MQ=255) tcaaAGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAg > 2:1092887/1‑140 (MQ=255) aaaGATAAATTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGcc < 1:1007343/140‑1 (MQ=255) aTTGCTGCCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTa > 2:496158/1‑140 (MQ=255) gctgcCACTGGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATg < 2:1140275/140‑1 (MQ=255) ctgcCACTGGGGGGCTGACGTGATATTTATTCCGGGTCACGGACCATTATCCACCCTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGc > 2:1005525/1‑140 (MQ=255) | TGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACT‑GGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGC > NC_000913/984057‑984325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |