| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,131,170 | (A)6→7 | coding (153/417 nt) | flgB → | flagellar basal‑body rod protein FlgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,131,164 | 1 | . | A | 97.3% | 125.4 / ‑3.5 | 37 | coding (147/417 nt) | flgB | flagellar basal‑body rod protein FlgB |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (13/23); total (13/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
ATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑AAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGAC > NC_000913/1131025‑1131290 | aTAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACtt > 1:825988/1‑140 (MQ=255)aTAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACtt > 1:898856/1‑140 (MQ=255) tAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑a > 2:410246/1‑140 (MQ=255) tAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑a > 2:923814/1‑140 (MQ=255) aaGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aa > 1:1133340/1‑140 (MQ=255) gCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaa > 1:184540/1‑139 (MQ=255) gCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaa < 2:184540/139‑1 (MQ=255) gCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaa > 1:481660/1‑139 (MQ=255) gCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaa < 2:481660/139‑1 (MQ=255) cTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaaa‑a < 1:970925/140‑1 (MQ=255) cTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaaa‑a < 1:410246/140‑1 (MQ=255) gccgccTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑aaaa‑aaagtca < 2:825988/140‑6 (MQ=255) cgccTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATg < 2:1133340/140‑1 (MQ=255) tttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc < 1:976653/140‑1 (MQ=255) tttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc < 1:415196/140‑1 (MQ=255) tttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc < 1:65658/140‑1 (MQ=255) tttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc < 2:1010914/140‑1 (MQ=255) tttCAACAAGAGGCGCTAAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc < 1:643713/140‑1 (MQ=255) agagGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCa < 2:1160957/140‑1 (MQ=255) agagGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCa < 2:979096/140‑1 (MQ=255) gcgcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCa > 2:947561/1‑140 (MQ=255) gcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGAt < 1:894434/131‑1 (MQ=255) gcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGAt > 2:894434/1‑131 (MQ=255) cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc < 1:1036149/123‑1 (MQ=255) cAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGAc > 2:1036149/1‑123 (MQ=255) aTCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGt < 1:974900/140‑1 (MQ=255) tCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGtt < 1:710010/140‑1 (MQ=255) cgcgcCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCAc > 2:658445/1‑140 (MQ=255) aGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGAt < 2:926102/140‑1 (MQ=255) agcaAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACAtt > 2:634252/1‑140 (MQ=255) tGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTg < 1:634252/140‑1 (MQ=255) tGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑AAAATAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTg < 2:662483/140‑1 (MQ=255) aTACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACg < 1:702967/138‑1 (MQ=255) aTACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACg > 2:702967/1‑138 (MQ=255) aTACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGcc > 2:763051/1‑140 (MQ=255) aTACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGcc > 2:883193/1‑140 (MQ=255) ccccTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGcctcc < 1:1035023/140‑1 (MQ=255) ccTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTa > 2:84781/1‑140 (MQ=255) tGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTAcc > 1:503948/1‑140 (MQ=255) tGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTAcc > 2:333579/1‑140 (MQ=255) tGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTAcc > 2:650470/1‑140 (MQ=255) ggTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCg < 1:530358/140‑1 (MQ=255) tATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAg < 2:996753/140‑1 (MQ=255) cgcgcgATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCa > 1:618409/1‑140 (MQ=255) cgcgcgATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCa < 2:503948/140‑1 (MQ=255) cgcgcgATATCGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCa < 1:650470/140‑1 (MQ=255) cGATTTTGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTAtt < 1:84781/140‑1 (MQ=255) tGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGAc < 1:587723/140‑1 (MQ=255) tGCCAGTGAACTTAAAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGAc < 2:618409/140‑1 (MQ=255) | ATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTT‑AAAA‑AAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACACATTCCGGCGCAGGCGCTGACGCCTCCTACCGCAGAACTGCAATACCGTATTCCGGAC > NC_000913/1131025‑1131290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |