Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	310,347	C→T	V109M (GTG→ATG)	ecpA ←	common pilus major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	310,347	0	C	T	100.0%	248.7 / NA	71	V109M (GTG→ATG)	ecpA	common pilus major subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/40);  total (31/40)

CCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTAAA  >  NC_000913/310215‑310482                                                                                                                                     |                                                                                                                                        ccTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCcaca                                                                                                                                  >  2:988004/1‑140 (MQ=255) ccTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCcaca                                                                                                                                  >  1:175074/1‑140 (MQ=255) ccTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCcaca                                                                                                                                  >  2:804455/1‑140 (MQ=255)   tgtgCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACAtt                                                                                                                                <  2:175074/140‑1 (MQ=255)   tgtgCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACAtt                                                                                                                                <  1:410506/140‑1 (MQ=255)     tgCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCa                                                                                                                              >  1:832172/1‑140 (MQ=255)       cGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAgt                                                                                                                            >  2:87474/1‑140 (MQ=255)         gtggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAgtgt                                                                                                                          <  1:654134/140‑1 (MQ=255)         gtggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAgtgt                                                                                                                          <  2:286602/140‑1 (MQ=255)          tggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGtgtg                                                                                                                         >  2:633394/1‑140 (MQ=255)          tggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGtgtg                                                                                                                         >  2:526630/1‑140 (MQ=255)          tggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCAGCGCCGTTATAATCCATGCCCACATTCAGtgtg                                                                                                                         >  2:97293/1‑140 (MQ=255)            gtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGa                                                                                                                       >  1:906055/1‑140 (MQ=255)             tACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGaa                                                                                                                      <  2:704419/140‑1 (MQ=255)              aCGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAAc                                                                                                                     <  1:483567/140‑1 (MQ=255)              aCGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAAc                                                                                                                     <  2:906055/140‑1 (MQ=255)                 aTTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTg                                                                                                                  <  1:804455/140‑1 (MQ=255)                 aTTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTg                                                                                                                  <  1:818981/140‑1 (MQ=255)                           ttGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCaa                                                                                                        <  2:382040/140‑1 (MQ=255)                           ttGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCaa                                                                                                        <  2:560651/140‑1 (MQ=255)                                aCCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTg                                                                                                                  >  2:527420/1‑125 (MQ=255)                                aCCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTg                                                                                                                  <  1:527420/125‑1 (MQ=255)                                 ccGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGt                                                                                                  <  1:537452/140‑1 (MQ=255)                                    ttAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACATGAGGTATCCAACTGGGTTaa                                                                                               <  2:832172/140‑1 (MQ=255)                                      aGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCaa                                                                                                        >  2:736540/1‑129 (MQ=255)                                      aGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCaa                                                                                                        <  1:736540/129‑1 (MQ=255)                                      aGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAAtg                                                                                             >  2:302933/1‑140 (MQ=255)                                        ccAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAAtgtg                                                                                           <  2:478159/140‑1 (MQ=255)                                            cGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTgg                                                                                       >  1:711004/1‑140 (MQ=255)                                            cGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTgg                                                                                       >  1:980102/1‑140 (MQ=255)                                            cGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCAACACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATgtgt                                                                                          >  2:920565/1‑137 (MQ=255)                                            cGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCAACACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATgtgt                                                                                          <  1:920565/137‑1 (MQ=255)                                                 tAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGAt                                                                                   <  2:668736/139‑1 (MQ=255)                                                 tAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGAt                                                                                   >  1:668736/1‑139 (MQ=255)                                                        gccgccCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTg                                                                           >  1:122527/1‑140 (MQ=255)                                                         ccgccCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATgtgt                                                                                          <  2:872904/124‑1 (MQ=255)                                                         ccgccCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATgtgt                                                                                          >  1:872904/1‑124 (MQ=255)                                                         ccgccCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGt                                                                            >  2:634028/1‑138 (MQ=255)                                                         ccgccCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGt                                                                            <  1:634028/138‑1 (MQ=255)                                                            cccAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGt                                                                       <  2:671664/140‑1 (MQ=255)                                                            cccAGTACGCCGTTGGCGGTACCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGt                                                                       <  2:1068935/140‑1 (MQ=255)                                                                    gCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTaa                                                               <  1:149729/140‑1 (MQ=255)                                                                               tATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGGGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGGAGcc                                                    >  1:221065/1‑140 (MQ=255)                                                                                aTCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCg                                                   >  2:1109320/1‑140 (MQ=255)                                                                                  cGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGtt                                                 >  2:417812/1‑140 (MQ=255)                                                                                     tcatcaCGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAg                                              <  1:86929/140‑1 (MQ=255)                                                                                         caCGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCAc                                          >  1:470035/1‑140 (MQ=255)                                                                                           cGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCAccc                                        >  2:695063/1‑140 (MQ=255)                                                                                             gTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCa                                           <  2:520367/135‑1 (MQ=255)                                                                                             gTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCa                                           >  1:520367/1‑135 (MQ=255)                                                                                              tATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAg                                              <  2:392816/131‑1 (MQ=255)                                                                                              tATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAg                                              >  1:392816/1‑131 (MQ=255)                                                                                                tCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGAt                                   >  1:1053766/1‑140 (MQ=255)                                                                                                  gCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAg                                 <  1:1109320/140‑1 (MQ=255)                                                                                                   ccAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGc                                >  2:574411/1‑140 (MQ=255)                                                                                                   ccAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGc                                >  1:1163413/1‑140 (MQ=255)                                                                                                    cAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGcc                               <  2:572697/140‑1 (MQ=255)                                                                                                    cAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGcc                               <  2:980102/140‑1 (MQ=255)                                                                                                    cAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGcc                               <  2:711004/140‑1 (MQ=255)                                                                                                    cAGTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGcc                               <  2:470035/140‑1 (MQ=255)                                                                                                      gTTTTTTCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCAc                             <  2:1053766/140‑1 (MQ=255)                                                                                                        tttttCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGt                           <  1:735008/140‑1 (MQ=255)                                                                                                          tttCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCa                         >  1:832565/1‑140 (MQ=255)                                                                                                           ttCGACTGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCaa                        <  1:748687/140‑1 (MQ=255)                                                                                                                 tGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGAc                  <  1:574411/140‑1 (MQ=255)                                                                                                                 tGCCGCGCCGTTATAATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGAc                  <  1:417812/140‑1 (MQ=255)                                                                                                                             ataATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCCCCCTCGATAGCCACGTCAAATAGACCTTTCt            <  1:767371/134‑1 (MQ=255)                                                                                                                             ataATCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCt            >  2:767371/1‑134 (MQ=255)                                                                                                                                 tCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTaaa  <  1:633394/140‑1 (MQ=255)                                                                                                                                 tCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTaaa  <  1:526630/140‑1 (MQ=255)                                                                                                                                 tCCATGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTaaa  <  2:150073/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCACGCCCACATTCAGTGTGGAACCTGAGGTATCCAACTGGGTTAATGTGTTGGTGATAAGACGTGAGGTCAGTTTAAAGGCGGTAGCCGTTGAGTCACCCTCGATAGCCACGTCAAATAGACCTTTCTGTGAGTTAAA  >  NC_000913/310215‑310482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑