Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,981	A→G	intergenic (+370/‑61)	yhdV → / → yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,981	0	A	G	100.0%	252.8 / NA	69	intergenic (+370/‑61)	yhdV/yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/32);  total (37/32)

GCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTG  >  NC_000913/3418852‑3419113                                                                                                                                  |                                                                                                                                     gcgcATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAg                                                                                                                            <  1:1164429/140‑1 (MQ=255)  cgcATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGa                                                                                                                           >  2:414681/1‑140 (MQ=255)    cATGTTTCATTTTCGCACCATTGCGGTGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACg                                                                                                                         <  1:1018430/140‑1 (MQ=255)    cATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACg                                                                                                                         >  2:438792/1‑140 (MQ=255)    cATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACg                                                                                                                         >  1:883601/1‑140 (MQ=255)            aTTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 <  2:1133067/140‑1 (MQ=255)            aTTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 <  1:683332/140‑1 (MQ=255)            aTTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATAGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 >  2:526636/1‑140 (MQ=255)             ttttCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaaa                                                                                                                <  2:891052/140‑1 (MQ=255)               ttCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCATAGGCAGGTAAAAGACGTTTCCCAAACg                                                                                                              <  2:478822/140‑1 (MQ=255)                tCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 <  1:132431/136‑1 (MQ=255)                tCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 >  2:132431/1‑136 (MQ=255)                 cGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTc                                                                                                            >  2:988257/1‑140 (MQ=255)                 cGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTc                                                                                                            >  1:777551/1‑140 (MQ=255)                   cACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGTAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGCATGGCCACAGGCAGGTAAAAGACGTCTCCCAAACGTCCt                                                                                                          >  2:811838/1‑140 (MQ=255)                     ccATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 >  2:858494/1‑131 (MQ=255)                     ccATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCaa                                                                                                                 <  1:858494/131‑1 (MQ=255)                                  cTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTc                                                                                           >  2:138518/1‑140 (MQ=255)                                  cTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTc                                                                                           >  2:1014401/1‑140 (MQ=255)                                  cTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTc                                                                                           >  2:53281/1‑140 (MQ=255)                                    gTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGc                                                                                         <  2:472189/140‑1 (MQ=255)                                    gTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGC‑TATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGcc                                                                                        <  1:464044/140‑1 (MQ=255)                                     tttttATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGcc                                                                                        <  2:283406/140‑1 (MQ=255)                                     tttttATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGcc                                                                                        <  1:803225/140‑1 (MQ=255)                                      ttttATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCt                                                                                       <  2:883601/140‑1 (MQ=255)                                       tttATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTc                                                                                      <  1:988257/140‑1 (MQ=255)                                        ttattaTTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTca                                                                                     <  1:247922/140‑1 (MQ=255)                                        ttattaTTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTca                                                                                     >  1:793946/1‑140 (MQ=255)                                         tattatTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTcac                                                                                    <  1:526636/140‑1 (MQ=255)                                            tattTCCTGTCTACACTCAGCTTATTGCTACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAgg                                                                                 <  1:1138545/140‑1 (MQ=255)                                            tattTCCTGTCTACACTCAGCTTATTGCTACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAgg                                                                                 <  1:1138544/140‑1 (MQ=255)                                            tattTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAgg                                                                                 <  1:414681/140‑1 (MQ=255)                                               ttCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAgg                                                                                 >  2:650645/1‑137 (MQ=255)                                               ttCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAgg                                                                                 <  1:650645/137‑1 (MQ=255)                                               ttCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAg                                                                              >  2:918755/1‑140 (MQ=255)                                                tCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGc                                                                             >  1:152235/1‑140 (MQ=255)                                                 ccTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCa                                                                            >  2:106500/1‑140 (MQ=255)                                                   tGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCAtt                                                                          >  2:679046/1‑140 (MQ=255)                                                      cTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATg                                                                       >  2:499296/1‑140 (MQ=255)                                                        acacTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCa                                                                            >  2:1001162/1‑133 (MQ=255)                                                        acacTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCa                                                                            <  1:1001162/133‑1 (MQ=255)                                                         cacTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGaaa                                                                    <  2:282349/140‑1 (MQ=255)                                                         cacTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGaaa                                                                    >  1:256087/1‑140 (MQ=255)                                                             cAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAga                                                                <  2:454263/140‑1 (MQ=255)                                                              aGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgat                                                               >  1:612383/1‑140 (MQ=255)                                                               gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatg                                                              >  1:652241/1‑140 (MQ=255)                                                               gCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatg                                                              >  1:738862/1‑140 (MQ=255)                                                                 ttATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatgat                                                            <  2:256087/140‑1 (MQ=255)                                                                  tATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatgatg                                                           <  1:808087/140‑1 (MQ=255)                                                                   aTTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAgatgatga                                                          >  2:631261/1‑140 (MQ=255)                                                                      gCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAg                                                       <  1:65665/140‑1 (MQ=255)                                                                          cgcgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCcac                                                   >  2:648287/1‑140 (MQ=255)                                                                            cgAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCacac                                                 <  2:542329/140‑1 (MQ=255)                                                                                      cgcAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCCCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTgccgc                                       <  1:53281/140‑1 (MQ=255)                                                                                         aaaaGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAg                                    >  1:210722/1‑140 (MQ=255)                                                                                           aaGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGTTGCCGCCAGCg                                  <  2:657240/140‑1 (MQ=255)                                                                                           aaGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAACCACACTGGCTGCCGCCAGCg                                  >  2:1069392/1‑140 (MQ=255)                                                                                                 gCACTACTTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGtgctgc                            <  1:631261/140‑1 (MQ=255)                                                                                                     tactTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGc                        <  1:679046/140‑1 (MQ=255)                                                                                                       ctTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCg                      >  2:431884/1‑140 (MQ=255)                                                                                                       ctTTTGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCg                      >  2:403992/1‑140 (MQ=255)                                                                                                           tGCTTATAAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGc                  >  2:158640/1‑140 (MQ=255)                                                                                                                 taAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCa            >  1:813545/1‑140 (MQ=255)                                                                                                                 taAGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCa            >  1:825638/1‑140 (MQ=255)                                                                                                                  aaGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAg           <  2:793946/140‑1 (MQ=255)                                                                                                                  aaGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAg           >  2:652169/1‑140 (MQ=255)                                                                                                                  aaGAGGATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAg           >  2:415566/1‑140 (MQ=255)                                                                                                                       gATGGCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCgct      >  2:1147570/1‑140 (MQ=255)                                                                                                                          ggCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCgctgct   >  2:716599/1‑140 (MQ=255)                                                                                                                          ggCCACAGGCAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGC‑TTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGctgctg  >  1:480197/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                     GCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGACAGGTAAAAGACGTTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTG  >  NC_000913/3418852‑3419113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑