Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,446,881	C→T	A2T (GCT→ACT)	rpsN ←	30S ribosomal subunit protein S14

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,446,881	0	C	T	100.0%	190.3 / NA	54	A2T (GCT→ACT)	rpsN	30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/30);  total (24/30)

TTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTC  >  NC_000913/3446745‑3447015                                                                                                                                         |                                                                                                                                       ttGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCCTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCAt                                                                                                                                     >  2:1138649/1‑140 (MQ=255)     gaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAg                                                                                                                                 <  2:1155279/140‑1 (MQ=255)     gaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAg                                                                                                                                 >  2:236616/1‑140 (MQ=255)     gaACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAg                                                                                                                                 >  2:398736/1‑140 (MQ=255)      aaCAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGt                                                                                                                                <  2:846974/140‑1 (MQ=255)         aGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAAc                                                                                                                             <  2:496015/140‑1 (MQ=255)         aGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAAc                                                                                                                             <  2:457570/140‑1 (MQ=255)             ttCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAACTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTa                                                                                                                         <  2:386163/140‑1 (MQ=255)               ccAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTAcc                                                                                                                       <  1:796647/140‑1 (MQ=255)                cAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCt                                                                                                                      <  2:600202/140‑1 (MQ=255)                     aTCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACtt                                                                                                                 >  1:526036/1‑140 (MQ=255)                        ttCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCg                                                                                                              >  2:755486/1‑140 (MQ=255)                               gAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACggg                                                                                                       <  1:1030011/140‑1 (MQ=255)                                 aGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGaa                                                                                                     <  2:237703/140‑1 (MQ=255)                                  gCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAg                                                                                                    >  2:150038/1‑140 (MQ=255)                                        acaTCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAg                                                                                              <  2:283641/140‑1 (MQ=255)                                            cAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcag                                                                                          >  2:453917/1‑140 (MQ=255)                                                 atgatCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGcagccagc                                                                                     >  1:489956/1‑140 (MQ=255)                                                      cGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAgc                                                                                >  2:493236/1‑140 (MQ=255)                                                        cTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAgcgc                                                                              <  1:755486/140‑1 (MQ=255)                                                              gttcagCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCctt                                                                        <  2:243656/140‑1 (MQ=255)                                                                   gcgcGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCg                                                                   >  2:218912/1‑140 (MQ=255)                                                                   gcgcGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCg                                                                   >  2:660940/1‑140 (MQ=255)                                                                    cgcgTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGt                                                                  >  1:151167/1‑140 (MQ=255)                                                                    cgcgTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGt                                                                  >  1:358111/1‑140 (MQ=255)                                                                    cgcgTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTAATTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGt                                                                  <  2:1093226/140‑1 (MQ=255)                                                                     gcgTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTc                                                                 <  2:164702/140‑1 (MQ=255)                                                                        tttCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGa                                                              <  1:218912/140‑1 (MQ=255)                                                                        tttCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGa                                                              <  2:489956/140‑1 (MQ=255)                                                                             cgAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCg                                                         <  1:28347/140‑1 (MQ=255)                                                                             cgAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCg                                                         <  1:370626/140‑1 (MQ=255)                                                                                 gTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCagt                                                     <  1:174637/140‑1 (MQ=255)                                                                                  tATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCagta                                                    <  2:330897/140‑1 (MQ=255)                                                                                         cAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaa                                             <  2:205366/140‑1 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGc                                                        <  2:78698/126‑1 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGc                                                        >  1:78698/1‑126 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCagta                                                    >  2:677312/1‑130 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCagta                                                    <  1:677312/130‑1 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatgg                                          >  1:917705/1‑140 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatgg                                          >  2:546304/1‑140 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatgg                                          >  1:244939/1‑140 (MQ=255)                                                                                            cTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGcggtaatgg                                          >  1:279387/1‑140 (MQ=255)                                                                                             tAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatggt                                         <  1:453917/140‑1 (MQ=255)                                                                                             tAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatggt                                         <  1:236616/140‑1 (MQ=255)                                                                                             tAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatggt                                         <  1:398736/140‑1 (MQ=255)                                                                                              aaaGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatggta                                        >  1:236341/1‑140 (MQ=255)                                                                                                        gTTTTACTTCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAAc                              <  2:236341/140‑1 (MQ=255)                                                                                                                tCGCGTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAAcg                      <  1:546304/140‑1 (MQ=255)                                                                                                                  gcgTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAAcgcg                    >  1:971450/1‑140 (MQ=255)                                                                                                                   cgTGCTTTCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCgg                   >  1:252994/1‑140 (MQ=255)                                                                                                                        tttCATTGATTGCTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGAc              <  2:358111/140‑1 (MQ=255)                                                                                                                                    cTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTc  <  2:526036/140‑1 (MQ=255)                                                                                                                                    cTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTc  >  2:555648/1‑140 (MQ=255)                                                                                                                                    cTTAGTCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTc  >  2:566624/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATGGTAATATCCAAACCACGAACGCGGTCGACTTTATCGTAGTC  >  NC_000913/3446745‑3447015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑