Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,698,498 T→C G95G (GGT→GGC)  bcsG → cellulose phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,698,4980TC98.5% 245.2 / ‑4.5 67G95G (GGT→GGC) bcsGcellulose phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (33/33);  minor base A (0/1);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTGCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGTTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGATTCGCATTACCGT  >  NC_000913/3698362‑3698632
                                                                                                                                        |                                                                                                                                      
ttGCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCg                                                                                                                                     >  1:812611/1‑140 (MQ=255)
 tGCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGc                                                                                                                                    >  1:316119/1‑140 (MQ=255)
  gCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGc                                                                                                                                    >  1:878932/1‑139 (MQ=255)
  gCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGc                                                                                                                                    <  2:878932/139‑1 (MQ=255)
  gCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCa                                                                                                                                   >  1:1119208/1‑140 (MQ=255)
  gCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCa                                                                                                                                   >  1:661003/1‑140 (MQ=255)
       gTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTgg                                                                                                                              <  2:812611/140‑1 (MQ=255)
         ttCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCg                                                                                                                            <  2:1120810/140‑1 (MQ=255)
         ttCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCg                                                                                                                            <  2:134149/140‑1 (MQ=255)
         ttCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCg                                                                                                                            <  2:538374/140‑1 (MQ=255)
             gctgATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGt                                                                                                                        <  1:681877/140‑1 (MQ=255)
              ctgATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGtt                                                                                                                       <  2:1119208/140‑1 (MQ=255)
              ctgATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGtt                                                                                                                       <  1:589243/140‑1 (MQ=255)
                   gCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTa                                                                                                                  >  1:429253/1‑140 (MQ=255)
                   gCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTa                                                                                                                  >  2:874843/1‑140 (MQ=255)
                        ttCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGat                                                                                                             >  2:938010/1‑140 (MQ=255)
                               cTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTaa                                                                                                      <  2:328233/140‑1 (MQ=255)
                               cTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTA‑CGattaaataat                                                                                                     <  2:275507/140‑7 (MQ=255)
                                tACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAAt                                                                                                     <  2:89053/140‑1 (MQ=255)
                                 aCAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATc                                                                                                    <  1:799687/140‑1 (MQ=255)
                                 aCAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATc                                                                                                    <  1:874843/140‑1 (MQ=255)
                                  cAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGt                                                                                                                   >  1:228565/1‑124 (MQ=255)
                                  cAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGt                                                                                                                   <  2:228565/124‑1 (MQ=255)
                                    gCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGAc                                                                                                 <  2:685967/140‑1 (MQ=255)
                                     ccTGCATCGCTTGCGCCACTGGATTGCCGTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGAcc                                                                                                >  1:281889/1‑140 (MQ=255)
                                          aTCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGAcc                                                                                                <  1:537162/135‑1 (MQ=255)
                                          aTCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGAcc                                                                                                >  2:537162/1‑135 (MQ=255)
                                             gCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTcaca                                                                                        <  1:938010/140‑1 (MQ=255)
                                              cTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCacac                                                                                       <  2:316119/140‑1 (MQ=255)
                                              cTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCacac                                                                                       >  2:938821/1‑140 (MQ=255)
                                              cTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCacac                                                                                       <  1:424929/140‑1 (MQ=255)
                                              cTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGACATGACACCTGGTTGCCTGGCCCGGAAAGCATAACGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCacac                                                                                       <  2:811317/140‑1 (MQ=255)
                                               ttGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACg                                                                                      >  1:256187/1‑140 (MQ=255)
                                                tGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTcaca                                                                                        >  1:657520/1‑137 (MQ=255)
                                                tGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTcaca                                                                                        <  2:657520/137‑1 (MQ=255)
                                                  cgcCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCACAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCtt                                                                                   >  1:289660/1‑140 (MQ=255)
                                                            ttGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTgg                                                                         >  2:394113/1‑140 (MQ=255)
                                                               cccTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCacac                                                                                       >  1:108659/1‑123 (MQ=255)
                                                               cccTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCacac                                                                                       <  2:108659/123‑1 (MQ=255)
                                                               cccTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAg                                                                      >  2:9083/1‑140 (MQ=255)
                                                               cccTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAg                                                                      <  1:430260/140‑1 (MQ=255)
                                                                    ccGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAgatgat                                                                 >  2:58490/1‑140 (MQ=255)
                                                                    ccGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAgatgat                                                                 >  1:810125/1‑140 (MQ=255)
                                                                       aTCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTg                                                                          <  2:657164/128‑1 (MQ=255)
                                                                       aTCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTg                                                                          >  1:657164/1‑128 (MQ=255)
                                                                          ggCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGc                                                           <  2:661003/140‑1 (MQ=255)
                                                                            ctttgctttgTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCa                                                         >  2:601986/1‑140 (MQ=255)
                                                                              ttgctttgTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCAtt                                                       >  2:855546/1‑140 (MQ=255)
                                                                                      ttCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGtttt                                               >  1:406313/1‑140 (MQ=255)
                                                                                           gCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAg                                          >  2:517040/1‑140 (MQ=255)
                                                                                               gACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGc                                      <  2:395695/140‑1 (MQ=255)
                                                                                               gACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGc                                      <  2:256187/140‑1 (MQ=255)
                                                                                                acacCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGcc                                     >  2:40278/1‑140 (MQ=255)
                                                                                                      ggTTGCCTGGCCCGGGAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTTACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTa                               >  2:930990/1‑140 (MQ=255)
                                                                                                       gTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTAt                              <  1:58490/140‑1 (MQ=255)
                                                                                                        ttGCCTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTAtt                             <  1:928032/140‑1 (MQ=255)
                                                                                                           ccTGGCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCt                          <  1:938821/140‑1 (MQ=255)
                                                                                                           ccTGGCCCGGAAAGCATAATGAGCCAGGGATCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCt                          <  1:451867/140‑1 (MQ=255)
                                                                                                              ggCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCt                          <  1:219816/137‑1 (MQ=255)
                                                                                                              ggCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCt                          >  2:219816/1‑137 (MQ=255)
                                                                                                              ggCCCGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTc                       >  2:847293/1‑140 (MQ=255)
                                                                                                                 ccGGAAAGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTcaca                    <  2:429755/140‑1 (MQ=255)
                                                                                                                       aGCATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGAt              <  2:427915/140‑1 (MQ=255)
                                                                                                                         cATAATGAGCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGATTc            >  1:356178/1‑140 (MQ=255)
                                                                                                                                 gCCAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGATTCGCATTAcc    >  2:907541/1‑140 (MQ=255)
                                                                                                                                   cAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGATTCGCATTACCGt  >  2:881694/1‑140 (MQ=255)
                                                                                                                                   cAGGGCTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGATTCGCATTACCGt  >  2:245216/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TTGCCGCGTTTCTGCTGATGCCCCTTCCGCGCTACAGCCTGCATCGCTTGCGCCACTGGATTGCCCTGCCGATCGGCTTTGCTTTGTTCTGGCATGACACCTGGTTGCCTGGCCCGGAAAGCATAATGAGCCAGGGTTCGCAGGTGGCGGGGTTCAGTACCGATTATTTAATCGACCTTGTCACACGCTTTATTAACTGGCAGATGATTGGGGCCATTTTTGTTTTATTAGTGGCCTGGTTATTCCTGTCACAATGGATTCGCATTACCGT  >  NC_000913/3698362‑3698632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑