Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	375,596	A→G	A46A (GCA→GCG)	mhpT →	3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,596	0	A	G	100.0%	86.3 / NA	25	A46A (GCA→GCG)	mhpT	3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/22);  total (3/22)

CTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAGCGCATTTTGATTGGCTCAGTTGCGCTGTTTGGT  >  NC_000913/375466‑375731                                                                                                                                   |                                                                                                                                        cTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCCGGCTTTTGCGCCCGATaaa                                                                                                                                >  2:199016/1‑140 (MQ=255)     tACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGGATCGCCCCGGCTTTCGCGCTCGGTAAAAAGc                                                                                                                            >  2:886842/1‑140 (MQ=255)       ccccTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGTCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGCTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCaa                                                                                                                          <  2:1037660/140‑1 (MQ=255)       ccccTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCaa                                                                                                                          <  1:668540/140‑1 (MQ=255)           ttCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATgg                                                                                                                      <  1:886842/140‑1 (MQ=255)            tcatcaTCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATggg                                                                                                                     <  2:500593/140‑1 (MQ=255)            tcatcaTCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAACggg                                                                                                                     <  1:1136423/140‑1 (MQ=255)             catcatCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGc                                                                                                                    <  1:851257/140‑1 (MQ=255)             catcatCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGACTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGc                                                                                                                    <  2:21947/140‑1 (MQ=255)                catcTTCATCCTGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCCCGGTAAAATGCAAAAGGGCTgg                                                                                                                 >  2:290921/1‑140 (MQ=255)                  tcTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGCTCGCCCCGGCTTTCGCGCTCGGTAAAATGCAAACGGGGTgggt                                                                                                               >  2:1136423/1‑138 (MQ=255)                     tCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATTCAAATgg                                                                                                                      >  1:126102/1‑130 (MQ=255)                     tCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATgg                                                                                                                      <  2:126102/130‑1 (MQ=255)                       aTCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTAGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGGCACCCCCCCGCTTTTCCGCTCCATAAAAATGAAATGGGCTGGGGAtttt                                                                                                          >  2:1092611/1‑139 (MQ=255)                          ccGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAgcg                                                                                                       <  1:1086957/140‑1 (MQ=255)                          ccGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAgcg                                                                                                       <  2:1042010/140‑1 (MQ=255)                            gCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGcc                                                                                                     <  1:611609/140‑1 (MQ=255)                             ccTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCg                                                                                                    >  1:1013869/1‑140 (MQ=255)                                      gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCg                                                                                           <  1:959859/140‑1 (MQ=255)                                             gggCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCt                                                                                    >  1:42887/1‑140 (MQ=255)                                                       ttttGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGcgcg                                                                          <  1:290921/140‑1 (MQ=255)                                                            gTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCgttggt                                                                     <  1:806167/140‑1 (MQ=255)                                                                    gATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGaa                                                             <  1:1092611/140‑1 (MQ=255)                                                                         aaGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTg                                                        <  1:512557/140‑1 (MQ=255)                                                                           gggCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGc                                                      <  2:66975/140‑1 (MQ=255)                                                                            ggCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCg                                                     <  2:181849/140‑1 (MQ=255)                                                                            ggCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCg                                                     <  2:42887/140‑1 (MQ=255)                                                                             gCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCgg                                                    <  2:280041/140‑1 (MQ=255)                                                                               tGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGGATCGCCCCGGCCTTTGCGCTCGGTAAAAAGCAAAAGGGGTGGGTATTTTGCGCCGGGATACTCGGGTTGCTACCCCGGGCGGTGGGTGGGGGGATGCTGGCgggc                                                  >  2:434944/1‑138 (MQ=255)                                                                               tGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCCGGCTTTTGCGCTCGGTAAAAAGCAAATGGGGTGGGTATTTAGCGCCGGGATACTCGGGTTGCTACCCCGCGCGGTGGTTGGGGGGATGCTGGCgggc                                                  >  2:512557/1‑138 (MQ=255)                                                                                            ggcTGGCATTGCGGCGGGTGGCATCGCCCAGGCCTTCGCGCTCGATAAAAAGCAAATGGGGTGGGTATTTAGCGCCGGAATACTCGGTTTGTTACCCGGCGCGCTGGTTGGCGGAATGTTGGCGGGCCGGTATGGTCGCa                                     >  2:229990/1‑140 (MQ=255)                                                                                             gcTGGCATTGCGGCGGGTGGCATCCCCCCGGCCTTCGCCCTCGATAAAATGCAAATGGGCTGGGTATTTATCGCCGGGATACTCCGGTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGGCCGTTTTGGTCGCaa                                    >  2:781775/1‑140 (MQ=255)                                                                                              cTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAg                                   <  2:1013869/140‑1 (MQ=255)                                                                                                                 cATCGCCCAGGCTTTCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAGCGCATTTTGATTGGCTCAg                >  1:1128748/1‑140 (MQ=255)                                                                                                                               tCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAGCGCATTTTGATTGGCTCAGTTGCGCTgtttggt  >  2:325388/1‑140 (MQ=255)                                                                                                                               tCGCGCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAGCGCATTTTGATTGGCTCAGTTGCGCTgtttggt  <  1:994612/140‑1 (MQ=255)                                                                                                                                cgcgCTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAGCGCATTTTGATTGGCTCAGTTg             <  1:951046/128‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CTCGTACCCCTTCATCATCTTCATCCCGCCTGATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAATGCTGGCGGACCGTTATGGTCGCAAGCGCATTTTGATTGGCTCAGTTGCGCTGTTTGGT  >  NC_000913/375466‑375731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑