Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,181,411	T→C	V56A (GTA→GCA)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,181,411	0	T	C	100.0%	302.1 / NA	81	V56A (GTA→GCA)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/35);  total (46/35)

TAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGT  >  NC_000913/4181280‑4181547                                                                                                                                    |                                                                                                                                         tAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGa                                                                                                                                  <  1:655723/140‑1 (MQ=255)     gATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGAtt                                                                                                                                >  1:818238/1‑138 (MQ=255)     gATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGAtt                                                                                                                                <  2:818238/138‑1 (MQ=255)            gTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTAc                                                                                                                       <  2:676781/140‑1 (MQ=255)            gTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTAc                                                                                                                       <  1:635163/140‑1 (MQ=255)            gTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTAc                                                                                                                       <  2:71136/140‑1 (MQ=255)                      acaAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAAtt                                                                                                             <  2:878491/140‑1 (MQ=255)                      acaAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAAtt                                                                                                             <  2:1012550/140‑1 (MQ=255)                         aGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCg                                                                                                          <  2:678640/140‑1 (MQ=255)                             cTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCt                                                                                                      >  1:85666/1‑140 (MQ=255)                               ggATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGc                                                                                                    <  1:1147268/140‑1 (MQ=255)                                gATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCa                                                                                                   <  2:1155917/140‑1 (MQ=255)                                gATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCa                                                                                                   <  1:838605/140‑1 (MQ=255)                                     aCCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACg                                                                                              >  2:550799/1‑140 (MQ=255)                                      ccTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGt                                                                                             <  2:372784/140‑1 (MQ=255)                                          aTCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGc                                                                                         >  1:967384/1‑140 (MQ=255)                                            ctcCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTa                                                                                       <  2:559146/140‑1 (MQ=255)                                               cTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGt                                                                                             >  2:269618/1‑131 (MQ=255)                                               cTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGt                                                                                             <  1:269618/131‑1 (MQ=255)                                                   cTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCtt                                                                                >  2:818553/1‑140 (MQ=255)                                                     aTCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTa                                                                                       <  2:170921/131‑1 (MQ=255)                                                     aTCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTa                                                                                       >  1:170921/1‑131 (MQ=255)                                                     aTCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTgg                                                                              >  2:1125742/1‑140 (MQ=255)                                                     aTCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTgg                                                                              >  2:805118/1‑140 (MQ=255)                                                     aTCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTgg                                                                              >  1:442989/1‑140 (MQ=255)                                                      tCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGc                                                                             >  1:412826/1‑140 (MQ=255)                                                      tCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGc                                                                             >  1:85601/1‑140 (MQ=255)                                                         aGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGaa                                                                          >  2:514813/1‑140 (MQ=255)                                                          gCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAAc                                                                         <  1:720194/140‑1 (MQ=255)                                                           cTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAAcc                                                                        >  2:492208/1‑140 (MQ=255)                                                                cTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAAc                                                                         <  2:907012/134‑1 (MQ=255)                                                                cTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAAc                                                                         >  1:907012/1‑134 (MQ=255)                                                                cTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGgtgt                                                                   >  1:545703/1‑140 (MQ=255)                                                                cTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGgtgt                                                                   >  2:126528/1‑140 (MQ=255)                                                                cTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGgtgt                                                                   >  2:596138/1‑140 (MQ=255)                                                                  cGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGttt                                                                 <  2:348228/140‑1 (MQ=255)                                                                   gTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTg                                                                <  1:985501/140‑1 (MQ=255)                                                                   gTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTg                                                                <  1:142947/140‑1 (MQ=255)                                                                    tttCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGa                                                               >  2:590502/1‑140 (MQ=255)                                                                     ttCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGGAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGAc                                                              >  2:570516/1‑140 (MQ=255)                                                                     ttCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGAc                                                              >  1:579819/1‑140 (MQ=255)                                                                        aGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTc                                                           <  1:596138/140‑1 (MQ=255)                                                                            aTTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAgg                                                       <  1:1125742/140‑1 (MQ=255)                                                                             tttATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGa                                                      >  2:862027/1‑140 (MQ=255)                                                                             tttATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGa                                                      <  2:579819/140‑1 (MQ=255)                                                                               tATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAAt                                                    >  2:681836/1‑140 (MQ=255)                                                                                        aGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATcc                                           <  1:483960/140‑1 (MQ=255)                                                                                             cTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGc                                      <  1:492208/140‑1 (MQ=255)                                                                                             cTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGc                                      <  2:283738/140‑1 (MQ=255)                                                                                               gAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGc                                      >  2:269841/1‑138 (MQ=255)                                                                                               gAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGc                                      <  1:269841/138‑1 (MQ=255)                                                                                               gAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGt                                    >  2:1007685/1‑140 (MQ=255)                                                                                                aaGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTg                                   <  1:590502/140‑1 (MQ=255)                                                                                                  gggCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGAc                                 <  1:1119038/140‑1 (MQ=255)                                                                                                   ggCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGTATGTCAAATCCGTGGCGTGAcc                                >  2:105127/1‑140 (MQ=255)                                                                                                   ggCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGAcc                                >  2:133281/1‑140 (MQ=255)                                                                                                   ggCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGAc                                 >  1:485683/1‑139 (MQ=255)                                                                                                   ggCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGAc                                 <  2:485683/139‑1 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATcc                                           <  2:176183/127‑1 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATcc                                           >  1:176183/1‑127 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGAc                                 >  2:180718/1‑137 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGAc                                 <  1:180718/137‑1 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTa                              >  1:1064207/1‑140 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTa                              >  2:349657/1‑140 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTa                              >  1:1120171/1‑140 (MQ=255)                                                                                                     cAGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTa                              >  2:114912/1‑140 (MQ=255)                                                                                                      aGTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTAt                             >  2:234218/1‑140 (MQ=255)                                                                                                       gTATGGTCTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTAtt                            <  2:1064207/140‑1 (MQ=255)                                                                                                              cTGGAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCAcc                     >  2:475747/1‑140 (MQ=255)                                                                                                                ggAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGGGGGAATTCCGAGCTGCAATACGTGCGCTACCGGCTTGGCGAACTGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGGGTGACCTATTCCGCACCGc                   >  2:1166447/1‑140 (MQ=255)                                                                                                                ggAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTa                              <  1:97279/129‑1 (MQ=255)                                                                                                                ggAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTa                              >  2:97279/1‑129 (MQ=255)                                                                                                                ggAAGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGc                   >  2:815268/1‑140 (MQ=255)                                                                                                                  aaGCTGCTTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTg                 >  1:1034309/1‑140 (MQ=255)                                                                                                                      tgctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGcgcg             >  2:309511/1‑140 (MQ=255)                                                                                                                      tgctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGcgcg             <  1:866630/140‑1 (MQ=255)                                                                                                                       gctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCt                  >  1:449041/1‑134 (MQ=255)                                                                                                                       gctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCt                  <  2:449041/134‑1 (MQ=255)                                                                                                                       gctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGt            >  1:811165/1‑140 (MQ=255)                                                                                                                       gctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGt            >  2:731630/1‑140 (MQ=255)                                                                                                                       gctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGt            >  1:1125804/1‑140 (MQ=255)                                                                                                                       gctTTCCGTTCCGCATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGt            >  2:349062/1‑140 (MQ=255)                                                                                                                           tccgttccgcATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCa                       <  2:21885/125‑1 (MQ=255)                                                                                                                           tccgttccgcATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCa                       >  1:21885/1‑125 (MQ=255)                                                                                                                           tccgttccgcATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTaaa        <  1:570516/140‑1 (MQ=255)                                                                                                                             cgttccgcATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACt      <  2:412826/140‑1 (MQ=255)                                                                                                                                 ccgcATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGt  <  2:264959/140‑1 (MQ=255)                                                                                                                                 ccgcATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGt  <  1:514813/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCAGAGCTACAGCGGTAATTCCGAGCTGCAATACGTCAGCTACCGCCTTGGCGAACCGGTGTTTGACGTCCAGGAATGTCAAATCCGTGGCGTGACCTATTCCGCACCGCTGCGCGTTAAACTGCGT  >  NC_000913/4181280‑4181547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑