Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,346,676	A→G	R217R (CGT→CGC)	fumB ←	fumarase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,346,676	0	A	G	100.0%	71.6 / NA	21	R217R (CGT→CGC)	fumB	fumarase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/16);  total (5/16)

TCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAT  >  NC_000913/4346551‑4346806                                                                                                                              |                                                                                                                                   tCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGGCGCCTTTTTTcgac                                                                                                                      >  1:1056639/1‑140 (MQ=255)    gTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGAGGGGGCGCCTTTTTTCGACGAg                                                                                                                   >  2:916767/1‑140 (MQ=255)         cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCCGTACCCAGGGGGCGCATTTTTTCGACGAGGGAgg                                                                                                              >  1:541319/1‑139 (MQ=255)                     aTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGc                                                                                                  <  1:814728/140‑1 (MQ=255)                           aGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCCGCAGGACCGAGGGGGCGCATTTTTTCGCCGAGGGAGGTTTTTACTTTGCCGGGAg                                                                                            >  2:814728/1‑140 (MQ=255)                           aGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGGGGCATTTTTTCTACGAGGAAGGTTTTTAGTTTTCCggggg                                                                                            >  2:1126958/1‑138 (MQ=255)                             cgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGTGCCGTACCGCGGGGGGGCCTTTTTTCCACGAGGAAGGTTTTTTGTTTTCCggggggc                                                                                          >  2:542837/1‑136 (MQ=255)                              gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGGGGCATTTTTTCGACGAGGGAGGTTTTTAGTTTGTCggggggcc                                                                                         >  2:197700/1‑135 (MQ=255)                                          gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTgg                                                                             <  1:197700/140‑1 (MQ=255)                                          gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTgg                                                                             <  2:848883/140‑1 (MQ=255)                                          gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTgg                                                                             <  1:968211/140‑1 (MQ=255)                                          gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGGCGCATTTTTTCGACGAGGAAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGCTTTgg                                                                             >  2:1009675/1‑140 (MQ=255)                                            cGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCAGGGGGGGCGTTTTTTCGACGGGGGAGATTTTTTGTTTGTCGGGGGTGAGCAGGGCTTTGGtt                                                                           >  2:152470/1‑140 (MQ=255)                                                tttCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCt                                                                       <  1:899525/140‑1 (MQ=255)                                                                gCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGTCGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtttt                                                       <  2:803150/140‑1 (MQ=255)                                                                    aCGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGCGGGGGGGCCTTTTTTCGACGAGGGAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGGTTTGGTTTTCTGGTAGAGATACGTTtttttg                                                   >  2:600509/1‑140 (MQ=255)                                                                       tACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCa                                                <  2:4389/140‑1 (MQ=255)                                                                         ccGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGGACCGAGGGGGGGCATTTTCTCGACGAGGGAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGGTTTGGTTTTCTGGGAGAGATACGTTTTTTTGGCagg                                              >  2:500213/1‑139 (MQ=255)                                                                             cAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccg                                          <  1:966310/140‑1 (MQ=255)                                                                                    aaaCGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGGGGCATTTTTTCGACGAGGGAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGCTTTTGTTTTCTGGTAGAGGTACGTTTTGTTGGGAGAgccgccgccct                                   >  2:966310/1‑138 (MQ=255)                                                                                           atatGGTACGGCGGGCAGGCCGCAGTACCGAGGGGGCGCATTTTTTCGCCGAGGAAGTTTTTTCGTTTGTCGGGGGGCAGCAGGGCTTTGGTTTTCTGGGAGAGATAAGTTTTGTTGGGGGAGCCGCCCCCTTTcgcaac                            >  2:714485/1‑140 (MQ=255)                                                                                              aggTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacgca                         <  1:207514/139‑1 (MQ=255)                                                                                              aggTACGGCGGGCAGGCTGCAGTACCAAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacgca                         <  1:822960/139‑1 (MQ=255)                                                                                                gTACGGCGGGCAGGCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCaaa                       <  2:69323/140‑1 (MQ=255)                                                                                                        ggCAGGCTGCAGTACCGGGGGGGCGCATTTTTTCGACGAGGAAGGTTTTTTGTTTGTCGGGGGTCAGCAGGGCTTTGGTTTTCTGGGAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTTGCCACGCAAAGGAAattt               >  2:667216/1‑139 (MQ=255)                                                                                                           aGGCTGCAGTACCGAGGGGGCGCCTTTTTTCGACGAGGGAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGGTTTTGGTTTCTGGGAGAGATACGTTTTTTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGtct            >  2:207514/1‑138 (MQ=255)                                                                                                           aGGCTGCAGGACCGAGGGGGCGCATTTTCTCGACGAGGGAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGGTTTGGGTTTCTGGTAGAGATACGTTTTGTTGGCAGAGCCCCCCCCCTTCGCAACGCAAAGGAAATTGtct            >  2:822960/1‑138 (MQ=255)                                                                                                             gCTGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCATGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTc          >  2:116360/1‑140 (MQ=255)                                                                                                               tGCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCAt        >  2:729997/1‑140 (MQ=255)                                                                                                                gCAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATc       >  2:348839/1‑140 (MQ=255)                                                                                                                 cAGTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCg      <  2:420063/140‑1 (MQ=255)                                                                                                                   gTACCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGcc    >  2:280812/1‑140 (MQ=255)                                                                                                                     aCCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt  <  2:1056639/140‑1 (MQ=255)                                                                                                                     aCCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt  <  1:714485/140‑1 (MQ=255)                                                                                                                     aCCGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCATGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt  <  1:116360/140‑1 (MQ=255)                                                                                                                       cGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt  <  1:91042/138‑1 (MQ=255)                                                                                                                       cGAGGGTGCGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAt  >  2:91042/1‑138 (MQ=255)                                                                                                                              |                                                                                                                                   TCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCAT  >  NC_000913/4346551‑4346806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑