Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,554,639	A→G	I22V (ATC→GTC)	uxuR →	DNA‑binding transcriptional repressor UxuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,554,639	0	A	G	100.0%	220.1 / NA	60	I22V (ATC→GTC)	uxuR	DNA‑binding transcriptional repressor UxuR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

TTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGATCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCAAAGGGCTGGTGGAA  >  NC_000913/4554503‑4554767                                                                                                                                         |                                                                                                                                 ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAAAACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCa                                                                                                                               >  2:656645/1‑140 (MQ=255)         cGGATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACg                                                                                                                       <  1:342230/140‑1 (MQ=255)         cGGATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACg                                                                                                                       <  1:99322/140‑1 (MQ=255)         cGGATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACg                                                                                                                       <  2:1007752/140‑1 (MQ=255)           gATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGcc                                                                                                                     >  2:425484/1‑140 (MQ=255)            aTAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCg                                                                                                                    >  1:614024/1‑140 (MQ=255)                ggCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACa                                                                                                                >  2:268479/1‑140 (MQ=255)                               tCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGc                                                                                                 <  1:707265/140‑1 (MQ=255)                                   gCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTgcc                                                                                             >  2:439569/1‑140 (MQ=255)                                   gCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTgcc                                                                                             >  1:609078/1‑140 (MQ=255)                                                    cGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTg                                                                            <  1:1100843/140‑1 (MQ=255)                                                    cGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTg                                                                            >  1:218663/1‑140 (MQ=255)                                                        ccTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGa                                                                        <  1:855653/140‑1 (MQ=255)                                                           aaCGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAAt                                                                     >  1:939101/1‑140 (MQ=255)                                                           aaCGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAAt                                                                     >  1:105058/1‑140 (MQ=255)                                                            aCGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATg                                                                    >  1:386681/1‑140 (MQ=255)                                                                  acaacaCCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGAt                                                              <  1:1077367/140‑1 (MQ=255)                                                                   caacaCCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATg                                                             >  2:144877/1‑140 (MQ=255)                                                                   caacaCCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATg                                                             >  2:819903/1‑140 (MQ=255)                                                                         cATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCAcgc                                                       <  2:393043/140‑1 (MQ=255)                                                                         cATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCAcgc                                                       >  1:1076259/1‑140 (MQ=255)                                                                          aTGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCAcgcg                                                      >  2:400129/1‑140 (MQ=255)                                                                          aTGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCAcgcg                                                      <  1:656645/140‑1 (MQ=255)                                                                            gAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGa                                                    <  2:39178/140‑1 (MQ=255)                                                                            gAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGa                                                    <  2:1169689/140‑1 (MQ=255)                                                                                tCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACggt                                                >  2:974352/1‑140 (MQ=255)                                                                                tCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACggt                                                >  2:426257/1‑140 (MQ=255)                                                                                tCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACggt                                                <  1:426257/140‑1 (MQ=255)                                                                                   gCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGATGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACg                                                  >  2:457166/1‑135 (MQ=255)                                                                                   gCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGATGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACg                                                  <  1:457166/135‑1 (MQ=255)                                                                                   gCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCgg                                                     <  1:152249/132‑1 (MQ=255)                                                                                   gCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCgg                                                     >  2:152249/1‑132 (MQ=255)                                                                                           tGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAg                                     >  1:34827/1‑140 (MQ=255)                                                                                            gcgcAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAgc                                    <  2:470093/140‑1 (MQ=255)                                                                                            gcgcAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAgc                                    <  1:22176/140‑1 (MQ=255)                                                                                            gcgcAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAgc                                    >  1:907550/1‑140 (MQ=255)                                                                                             cgcAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAgcg                                   <  1:144877/140‑1 (MQ=255)                                                                                             cgcAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAgcg                                   >  2:400819/1‑140 (MQ=255)                                                                                             cgcAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAgcg                                   <  1:268479/140‑1 (MQ=255)                                                                                                 aaGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGa                               <  2:386681/140‑1 (MQ=255)                                                                                                  aGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGAt                              <  1:425484/140‑1 (MQ=255)                                                                                                   gACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATc                             <  2:1008450/140‑1 (MQ=255)                                                                                                    aCCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCa                            <  2:1076259/140‑1 (MQ=255)                                                                                                      cTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATg                          <  1:400819/140‑1 (MQ=255)                                                                                                          ccAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTgg                      <  2:105058/140‑1 (MQ=255)                                                                                                           cAGGAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTTATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGga                     <  1:431329/140‑1 (MQ=255)                                                                                                              gAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCt                        >  2:227707/1‑134 (MQ=255)                                                                                                              gAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCt                        <  1:227707/134‑1 (MQ=255)                                                                                                              gAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGAt                  >  1:20619/1‑140 (MQ=255)                                                                                                              gAAGTCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGAt                  >  1:580619/1‑140 (MQ=255)                                                                                                                  tCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCa                            >  2:517825/1‑126 (MQ=255)                                                                                                                  tCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCa                            <  1:517825/126‑1 (MQ=255)                                                                                                                  tCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCaaa              >  2:400086/1‑140 (MQ=255)                                                                                                                  tCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCaaa              >  2:526905/1‑140 (MQ=255)                                                                                                                  tCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCaaa              >  2:551121/1‑140 (MQ=255)                                                                                                                  tCGGGGCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCaaa              >  2:952796/1‑140 (MQ=255)                                                                                                                      ggCGATGATCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCAAAGGGc          <  2:609078/140‑1 (MQ=255)                                                                                                                          atgatCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCAAAGGGCtggt      <  2:580619/140‑1 (MQ=255)                                                                                                                            gatCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCAAAGGGCtggtgg    >  2:1033331/1‑140 (MQ=255)                                                                                                                              tCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCTAAGGGCTGGTGGaa  <  2:210007/140‑1 (MQ=255)                                                                                                                              tCCGCGATCTGGTCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCAAAGGGCTGGTGGaa  <  2:1123134/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 TTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAATTTGATTAACCGCACCTAACGGACACAACACCATGAAATCTGCCACCTCTGCGCAAAGACCTTACCAGGAAGTCGGGGCGATGATCCGCGATCTGATCATAAAGACGCCGTACAATCCTGGCGAACGGCTGCCGCCGGAGCGTGAAATTGCAGAAATGCTTGATGTCACGCGGACGGTGGTACGTGAAGCGCTGATCATGCTGGAGATCAAAGGGCTGGTGGAA  >  NC_000913/4554503‑4554767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑