Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	427,480	A→G	N65S (AAC→AGC)	yajC →	Sec translocon accessory complex subunit YajC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	427,480	0	A	G	100.0%	240.2 / NA	65	N65S (AAC→AGC)	yajC	Sec translocon accessory complex subunit YajC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/28);  total (37/28)

CCCGATGTCTTTGATTTTGATGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGG  >  NC_000913/427343‑427611                                                                                                                                          |                                                                                                                                    cccGATGTCTTTGATTTTGATGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCg                                                                                                                                   >  2:145826/1‑140 (MQ=255)      tGTCTTTGATTTTGATGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGc                                                                                                                              >  1:536254/1‑140 (MQ=255)        tCTTTGATTTTGATGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCt                                                                                                                            >  2:765213/1‑140 (MQ=255)          tttgattttgatGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCtgg                                                                                                                          >  1:375746/1‑140 (MQ=255)             gattttgatGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCtggttg                                                                                                                       <  1:152920/140‑1 (MQ=255)                 ttgatGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTcg                                                                                                                   <  2:119814/140‑1 (MQ=255)                  tgatGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTcgc                                                                                                                  <  1:949398/140‑1 (MQ=255)                  tgatGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTcgc                                                                                                                  <  2:190450/140‑1 (MQ=255)                            ggtATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAg                                                                                                        <  2:1140397/140‑1 (MQ=255)                                ttCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTACCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGc                                                                                                    >  1:496033/1‑140 (MQ=255)                                   ggTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGa                                                                                                 >  2:392410/1‑140 (MQ=255)                                    gccTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGaa                                                                                                <  2:363277/138‑1 (MQ=255)                                    gTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGaa                                                                                                >  1:1160395/1‑140 (MQ=255)                                    gTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATAGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGaa                                                                                                >  2:1068159/1‑140 (MQ=255)                                     tCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGaaa                                                                                               >  2:987917/1‑140 (MQ=255)                                        gATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACg                                                                                            >  2:304519/1‑140 (MQ=255)                                          ttttCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGc                                                                                          <  2:375746/140‑1 (MQ=255)                                            ttCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTa                                                                                        <  1:394860/140‑1 (MQ=255)                                                   tCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCt                                                                                 >  2:654690/1‑140 (MQ=255)                                                   tCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCt                                                                                 >  2:73423/1‑140 (MQ=255)                                                    cATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTa                                                                                <  1:1122895/140‑1 (MQ=255)                                                         tCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATcgcg                                                                           >  2:415708/1‑140 (MQ=255)                                                         tCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATcgcg                                                                           <  1:73423/140‑1 (MQ=255)                                                             gCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGa                                                                       >  1:781373/1‑140 (MQ=255)                                                              cGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATcg                                                                             <  2:330840/133‑1 (MQ=255)                                                              cGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATcg                                                                             >  1:330840/1‑133 (MQ=255)                                                              cGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGaa                                                                      >  2:235740/1‑140 (MQ=255)                                                              cGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGaa                                                                      >  1:337678/1‑140 (MQ=255)                                                              cGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTCGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGaa                                                                      >  1:1083909/1‑140 (MQ=255)                                                                tCCACAGCAGAAGCGCACCAAATAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATg                                                                    >  2:1127313/1‑140 (MQ=255)                                                                tCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATg                                                                    >  1:694539/1‑140 (MQ=255)                                                                 ccACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGa                                                                   <  2:694539/140‑1 (MQ=255)                                                                   acaGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACTGCTACATTGCTATCGCGCTGAATGaca                                                                 >  2:601068/1‑140 (MQ=255)                                                                   acaGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGaca                                                                 >  2:711768/1‑140 (MQ=255)                                                                    cagcagAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGacac                                                                <  1:304519/140‑1 (MQ=255)                                                                     agcagAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGAcacc                                                               <  2:141623/140‑1 (MQ=255)                                                                            gcgcACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAag                                                        <  1:765213/140‑1 (MQ=255)                                                                            gcgcACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAag                                                        <  2:781373/140‑1 (MQ=255)                                                                               cACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagtag                                                     >  2:758783/1‑140 (MQ=255)                                                                                 ccAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagta                                                      <  2:430158/137‑1 (MQ=255)                                                                                 ccAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagta                                                      >  1:430158/1‑137 (MQ=255)                                                                                       aaCACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTaaa                                             <  1:402570/140‑1 (MQ=255)                                                                                         cacaAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACg                                           >  1:285708/1‑140 (MQ=255)                                                                                          acaAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACTGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt                                          <  1:601068/140‑1 (MQ=255)                                                                                            aaaaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGa                                        <  2:285708/140‑1 (MQ=255)                                                                                              aaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACt                                      >  1:769931/1‑140 (MQ=255)                                                                                                     aTGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGc                               >  2:58576/1‑140 (MQ=255)                                                                                                          cTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCg                          >  1:1017280/1‑140 (MQ=255)                                                                                                          cTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCg                          >  1:168234/1‑140 (MQ=255)                                                                                                          cTCCATTGCCAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCg                          >  2:827082/1‑140 (MQ=255)                                                                                                                  ccAAAGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCg                  <  1:58576/140‑1 (MQ=255)                                                                                                                    aaaGGTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGaa                <  2:769931/140‑1 (MQ=255)                                                                                                                       ggTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg             >  1:284615/1‑140 (MQ=255)                                                                                                                       ggTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg             <  1:71139/140‑1 (MQ=255)                                                                                                                        gTGATGAAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGc            <  2:284615/140‑1 (MQ=255)                                                                                                                           atgaAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGaa                >  2:759569/1‑133 (MQ=255)                                                                                                                           atgaAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGaa                <  1:759569/133‑1 (MQ=255)                                                                                                                            tgaAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCa        >  2:373953/1‑140 (MQ=255)                                                                                                                            tgaAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCa        >  2:670199/1‑140 (MQ=255)                                                                                                                            tgaAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCa        >  2:804216/1‑140 (MQ=255)                                                                                                                            tgaAGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCa        >  2:1074634/1‑140 (MQ=255)                                                                                                                               aGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGa     <  1:392410/140‑1 (MQ=255)                                                                                                                               aGTTCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGa     <  1:670199/140‑1 (MQ=255)                                                                                                                                  tCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg             <  2:961324/129‑1 (MQ=255)                                                                                                                                  tCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg             >  1:961324/1‑129 (MQ=255)                                                                                                                                  tCTGACGAGCGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAgg  >  1:1093797/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CCCGATGTCTTTGATTTTGATGCTGGTGGTATTCGGTCTGATTTTCTATTTCATGATCCTGCGTCCACAGCAGAAGCGCACCAAAGAACACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGG  >  NC_000913/427343‑427611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑