Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,564,136	A→G	V185A (GTG→GCG)	yjiL ←	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,564,136	0	A	G	100.0%	271.8 / NA	73	V185A (GTG→GCG)	yjiL	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/35);  total (38/35)

TACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCT  >  NC_000913/4564000‑4564272                                                                                                                                         |                                                                                                                                         tACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAGACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTc                                                                                                                                       >  2:1067525/1‑140 (MQ=255)     ggCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGc                                                                                                                                   >  1:125786/1‑140 (MQ=255)      gCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCg                                                                                                                                  >  1:1073561/1‑140 (MQ=255)      gCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAACGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCg                                                                                                                                  >  1:425232/1‑140 (MQ=255)       cATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCga                                                                                                                                 >  1:570871/1‑140 (MQ=255)       cATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCga                                                                                                                                 >  1:875349/1‑140 (MQ=255)                gTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCtt                                                                                                                        >  2:1075031/1‑140 (MQ=255)                 tGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTc                                                                                                                       >  1:956218/1‑140 (MQ=255)                   agaTTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTg                                                                                                                     >  1:450938/1‑140 (MQ=255)                       tCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGgcgc                                                                                                                 <  2:824231/140‑1 (MQ=255)                              tCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGccc                                                                                                          >  1:914593/1‑140 (MQ=255)                              tCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGccc                                                                                                          >  2:92879/1‑140 (MQ=255)                                 gggCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCt                                                                                                       >  1:675178/1‑140 (MQ=255)                                    cAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAg                                                                                                    <  1:876363/140‑1 (MQ=255)                                               cAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGAt                                                                                         <  1:970222/140‑1 (MQ=255)                                               cAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGAt                                                                                         <  2:914593/140‑1 (MQ=255)                                                        aCGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTc                                                                                   <  2:711299/137‑1 (MQ=255)                                                        aCGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTc                                                                                   >  1:711299/1‑137 (MQ=255)                                                           ccaccaGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTc                                                                             <  2:216881/140‑1 (MQ=255)                                                             accaGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAg                                                                           >  1:122463/1‑140 (MQ=255)                                                              ccaGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGTCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAgt                                                                          >  1:587871/1‑139 (MQ=255)                                                              ccaGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGc                                                                          >  1:748530/1‑140 (MQ=255)                                                              ccaGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGc                                                                          >  2:1054841/1‑140 (MQ=255)                                                               caGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGATGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCa                                                                         <  1:1131404/140‑1 (MQ=255)                                                                aGTAAACAGAATCGGCGCTTCACGGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCaa                                                                        <  2:1044230/140‑1 (MQ=255)                                                                aGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCaa                                                                        <  1:88530/140‑1 (MQ=255)                                                                aGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCaa                                                                        <  2:875349/140‑1 (MQ=255)                                                                  tAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAac                                                                      <  1:1054841/140‑1 (MQ=255)                                                                   aaaCAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAaca                                                                     <  1:1067525/140‑1 (MQ=255)                                                                     aCAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACt                                                                   <  2:570871/140‑1 (MQ=255)                                                                      cAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACtg                                                                  <  2:425232/140‑1 (MQ=255)                                                                         aaTCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAt                                                                               <  2:910500/124‑1 (MQ=255)                                                                         aaTCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGAt                                                                               >  1:910500/1‑124 (MQ=255)                                                                         aaTCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGc                                                               >  1:1144632/1‑140 (MQ=255)                                                                           tCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGTCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGTAAACACTGTGcac                                                             <  2:587871/140‑1 (MQ=255)                                                                            cGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGcaca                                                            >  2:98403/1‑140 (MQ=255)                                                                              gcgcTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATa                                                          >  2:996730/1‑140 (MQ=255)                                                                                   tCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCC‑‑CTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACtgt                                                                 <  2:20601/126‑1 (MQ=255)                                                                                   tCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCC‑‑CTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACtgt                                                                 >  1:20601/1‑126 (MQ=255)                                                                                    cacaGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTg                                                    <  2:125786/140‑1 (MQ=255)                                                                                            agaCGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTg                                            <  2:450938/140‑1 (MQ=255)                                                                                            agaCGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTg                                            >  2:888129/1‑140 (MQ=255)                                                                                            agaCGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTg                                            >  1:1032484/1‑140 (MQ=255)                                                                                              aCGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTgcg                                          >  1:149303/1‑140 (MQ=255)                                                                                              aCGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTgcg                                          >  2:541400/1‑140 (MQ=255)                                                                                                 aGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTgcggcg                                       >  2:588250/1‑140 (MQ=255)                                                                                                  gCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGt                                      <  2:675178/140‑1 (MQ=255)                                                                                                   cAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTg                                     >  2:493063/1‑140 (MQ=255)                                                                                                   cAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTg                                     <  1:771467/140‑1 (MQ=255)                                                                                                   cAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTg                                     >  1:15110/1‑140 (MQ=255)                                                                                                   cAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTg                                     <  2:1073561/140‑1 (MQ=255)                                                                                                   cAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTg                                     >  1:21872/1‑140 (MQ=255)                                                                                                   cAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTg                                     <  1:493063/140‑1 (MQ=255)                                                                                                        aaaTTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACAtt                                <  2:21872/140‑1 (MQ=255)                                                                                                        aaaTTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACAtt                                >  1:872624/1‑140 (MQ=255)                                                                                                        aaaTTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACAtt                                >  1:1066038/1‑140 (MQ=255)                                                                                                        aaaTTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACAtt                                <  1:541400/140‑1 (MQ=255)                                                                                                        aaaTTGGCACTCCTCCGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACAtt                                <  2:15110/140‑1 (MQ=255)                                                                                                                  tcctccGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATg                      >  1:369098/1‑140 (MQ=255)                                                                                                                    ctccGCGCCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCt                    >  1:664357/1‑140 (MQ=255)                                                                                                                          gcCATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCAGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAg              <  1:297715/140‑1 (MQ=255)                                                                                                                           ccATCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGt             <  2:664357/140‑1 (MQ=255)                                                                                                                              tCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGc          >  2:516199/1‑140 (MQ=255)                                                                                                                              tCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGc          >  1:678149/1‑140 (MQ=255)                                                                                                                              tCGCGTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGc          >  1:693154/1‑140 (MQ=255)                                                                                                                               cgcgTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTTAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCt         >  1:993832/1‑140 (MQ=255)                                                                                                                                gcgTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTc        <  1:588250/140‑1 (MQ=255)                                                                                                                                gcgTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTc        <  1:798326/140‑1 (MQ=255)                                                                                                                                  gTTAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGa      <  2:199018/140‑1 (MQ=255)                                                                                                                                    tAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACg    <  2:693154/140‑1 (MQ=255)                                                                                                                                    tAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACg    <  1:92879/140‑1 (MQ=255)                                                                                                                                    tAATCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACg    <  1:90844/140‑1 (MQ=255)                                                                                                                                      aTCGCTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCt  <  2:872624/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCGCGCCATCGCGTTAATCACTCCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCT  >  NC_000913/4564000‑4564272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑